Restriction map for
pF1KB3373
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BglI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FauI FseI FspI HgaI Hin1I
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SbfI ScaI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AciI 1675 CCGC CCGC(-3/-1)
AflIII 1838 ACATGT A^C[GA][CT]GT
AhdI 1527 GACCATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 1527 GACCATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1052 GAGCTC G[AT]GC[AT]^C
AvrII 703 CCTAGG C^CTAGG
BalI 1086 TGGCCA TGG^CCA
BcnI 533 CCCGG CC^[GC]GG
BlnI 703 CCTAGG C^CTAGG
BsaBI 724 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1052 GAGCTC G[AT]GC[AT]^C
BsmI 698 GAATGC GAATGC(1/-1)
BspLU11I 1838 ACATGT A^CATGT
BstAPI 1696 GCAATGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1301 CCATATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1711 TTTAAA TTT^AAA
EaeI 1086 TGGCCA [CT]^GGCC[GA]
Eam1105I 1527 GACCATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1447 GATATC GAT^ATC
HaeII 1357 GGCGCT [GA]GCGC^[CT]
HapII 534 CCGG C^CGG
HincII 62 GTCGAC GT[CT]^[GA]AC
HindII 62 GTCGAC GT[CT]^[GA]AC
HindIII 14 AAGCTT A^AGCTT
HpaII 534 CCGG C^CGG
MaeII 1487 ACGT A^CGT
MamI 724 GATAATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1086 TGGCCA TGG^CCA
MscI 1086 TGGCCA TGG^CCA
MspA1I 1817 CAGCTG C[AC]G^C[GT]G
MspI 534 CCGG C^CGG
NciI 533 CCCGG CC^[GC]GG
PflMI 594 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 134 GAGTC GAGTC(4/5)
PvuII 1817 CAGCTG CAG^CTG
SacI 1052 GAGCTC GAGCT^C
SalI 62 GTCGAC G^TCGAC
SapI 336 GAAGAGC GCTCTTC(1/4)
SexAI 524 ACCAGGT A^CC[AT]GGT
Van91I 594 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 62 GTCGAC GT^[AC][GT]AC
1108 GTCTAC
AfaI 371 GTAC GT^AC
1646 GTAC
AflII 921 CTTAAG C^TTAAG
1830 CTTAAG
AlwNI 1222 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1396 CAGATTCTG
AvaI 1049 CTCGAG C^[CT]CG[GA]G
1769 CTCGAG
BamHI 603 GGATCC G^GATCC
1278 GGATCC
BclI 6 TGATCA T^GATCA
912 TGATCA
BfrI 921 CTTAAG C^TTAAG
1830 CTTAAG
BglII 198 AGATCT A^GATCT
205 AGATCT
BseRI 491 CTCCTC GAGGAG(10/8)
152 GAGGAG
BsmFI 874 GGGAC GGGAC(10/14)
1543 GGGAC
BsoBI 1049 CTCGAG C^[CT]CG[GA]G
1769 CTCGAG
BsrDI 395 CATTGC GCAATG(2/0)
1696 GCAATG
Cac8I 12 GCAAGC GC[ACGT]^[ACGT]GC
1510 GCCAGC
CfoI 1358 GCGC GCG^C
1457 GCGC
DraII 465 AGGTCCT [GA]G^G[ACGT]CC[CT]
865 AGGACCC
EarI 336 GAAGAG CTCTTC(1/4)
1136 GAAGAG
EcoO109I 465 AGGTCCT [GA]G^G[ACGT]CC[CT]
865 AGGACCC
FbaI 6 TGATCA T^GATCA
912 TGATCA
HhaI 1358 GCGC GCG^C
1457 GCGC
HinP1I 1358 GCGC G^CGC
1457 GCGC
HphI 109 TCACC GGTGA(8/7)
1045 TCACC
Ksp632I 336 GAAGAG CTCTTC(1/4)
1136 GAAGAG
NdeI 331 CATATG CA^TATG
1352 CATATG
NspI 1642 GCATGT [GA]CATG^[CT]
1838 ACATGT
PaeR7I 1049 CTCGAG C^TCGAG
1769 CTCGAG
PpuMI 465 AGGTCCT [GA]G^G[AT]CC[CT]
865 AGGACCC
RsaI 371 GTAC GT^AC
1646 GTAC
TspRI 561 CAGTG CA[GC]TG[ACGT][ACGT]^
1599 CAGTG
XhoI 1049 CTCGAG C^TCGAG
1769 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BanII BbvI BfaI BmyI BpmI
BsaI BsaJI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EcoNI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SmlI SpeI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TthHB8I XcmI XhoII