Restriction map for
pF1KB3063
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AhdI ApaI AscI Asp718I AspEI
AviII AvrII BalI BamHI BbrPI
BciVI BclI BfrI BglII BlnI
BlpI Bpu1102I BsaBI BseAI BsiEI
BsiWI BsmBI BsmI BspDI BspEI
BspHI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MflI MluI MluNI
MroI MscI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RcaI
RsrII SacI SacII SalI SapI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SwaI XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 928 GTAGAC GT^[AC][GT]AC
AclI 300 AACGTT AA^CGTT
AcyI 1308 GGCGCC G[GA]^CG[CT]C
Alw44I 854 GTGCAC G^TGCAC
AlwI 1247 GGATC GGATC(4/5)
AlwNI 626 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 958 CCTCAGG CC^T[ACGT]AGG
ApaLI 854 GTGCAC G^TGCAC
Asp700I 126 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1786 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 1308 GGCGCC GGCGC^C
BbvCI 663 CCTCAGC CCTCAGC(-5/-2)
BfaI 715 CTAG C^TAG
BglI 2069 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1024 TACGTG [CT]AC^GT[GA]
BsaHI 1308 GGCGCC G[GA]^CG[CT]C
BsaWI 1517 ACCGGA [AT]^CCGG[AT]
Bsp1407I 32 TGTACA T^GTACA
BspLU11I 85 ACATGT A^CATGT
BsrGI 32 TGTACA T^GTACA
Bsu36I 958 CCTCAGG CC^T[ACGT]AGG
Eco81I 958 CCTCAGG CC^T[ACGT]AGG
EcoT14I 608 CCATGG C^C[AT][AT]GG
Hin1I 1308 GGCGCC G[GA]^CG[CT]C
KasI 1308 GGCGCC G^GCGCC
MaeI 715 CTAG C^TAG
NarI 1308 GGCGCC GG^CGCC
NcoI 608 CCATGG C^CATGG
PflFI 1786 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1486 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 300 AACGTT AA^CGTT
SbfI 1974 CCTGCAGG CCTGCA^GG
SfoI 1308 GGCGCC GGC^GCC
SmaI 1949 CCCGGG CCC^GGG
SmlI 637 CTCAAG C^T[CT][GA]AG
SphI 1919 GCATGC GCATG^C
Sse8387I 1974 CCTGCAGG CCTGCA^GG
SspBI 32 TGTACA T^GTACA
SspI 868 AATATT AAT^ATT
StuI 1416 AGGCCT AGG^CCT
StyI 608 CCATGG C^C[AT][AT]GG
Tth111I 1786 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1486 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2048 CCATGACGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1949 CCCGGG C^CCGGG
XmaI 1949 CCCGGG C^CCGGG
XmnI 126 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 981 CGCG CG^CG
1807 CGCG
AflIII 85 ACATGT A^C[GA][CT]GT
1661 ACGTGT
Aor51HI 1068 AGCGCT AGC^GCT
1236 AGCGCT
AseI 51 ATTAAT AT^TAAT
322 ATTAAT
AsnI 51 ATTAAT AT^TAAT
322 ATTAAT
BanI 697 GGCACC G^G[CT][GA]CC
1308 GGCGCC
BbsI 1543 GAAGAC GAAGAC(2/6)
1588 GTCTTC
BcgI 1959 CGACGTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
834 GCATATTCTTCG
BpuAI 1543 GAAGAC GAAGAC(2/6)
1588 GTCTTC
BspMI 1177 ACCTGC ACCTGC(4/8)
1977 GCAGGT
BsrDI 331 CATTGC GCAATG(2/0)
442 GCAATG
BstUI 981 CGCG CG^CG
1807 CGCG
BstXI 1052 CCAAAGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1404 CCAGGGTCCTGG
DraII 962 AGGTCCT [GA]G^G[ACGT]CC[CT]
1407 GGGTCCT
DsaI 608 CCATGG C^C[GA][CT]GG
689 CCGTGG
EaeI 611 TGGCCG [CT]^GGCC[GA]
1882 TGGCCG
Eco47III 1068 AGCGCT AGC^GCT
1236 AGCGCT
EcoNI 65 CCTATGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
636 CCTCAAGGAGG
EcoO109I 962 AGGTCCT [GA]G^G[ACGT]CC[CT]
1407 GGGTCCT
EcoRI 130 GAATTC G^AATTC
258 GAATTC
FauI 982 GCGGG CCCGC(4/6)
1305 GCGGG
MaeIII 116 GTGAC ^GT[ACGT]AC
1873 GTGAC
MslI 1251 CATCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2013 CACCGAGGTG
MvnI 981 CGCG CG^CG
1807 CGCG
PpuMI 962 AGGTCCT [GA]G^G[AT]CC[CT]
1407 GGGTCCT
PshBI 51 ATTAAT AT^TAAT
322 ATTAAT
PvuII 1798 CAGCTG CAG^CTG
2086 CAGCTG
SexAI 1224 ACCTGGT A^CC[AT]GGT
1876 ACCAGGT
TfiI 96 GATTC G^A[AT]TC
1611 GATTC
Tsp45I 116 GTGAC ^GT[GC]AC
1873 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaI AvaII BanII BbvI
BcnI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I