Restriction map for
pF1KB3046
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# NO CUT ENZYMES ( 101 enzymes) #
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AatII AccIII AclI AcyI AflII
AgeI AlwNI AocI Aor51HI AscI
AseI AsnI AspI AviII BbeI
BbrPI BclI BfrI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI Bsp1407I BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
CfoI Cfr10I CpoI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HpaI KasI KspI
MroI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AhdI 2599 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 745 GTGCAC G^TGCAC
ApaI 3536 GGGCCC GGGCC^C
ApaLI 745 GTGCAC G^TGCAC
AspEI 2599 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2247 CCTAGG C^CTAGG
BamHI 600 GGATCC G^GATCC
BglI 2087 GCCACTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2532 AGATCT A^GATCT
BlnI 2247 CCTAGG C^CTAGG
BspDI 1725 ATCGAT AT^CGAT
BspMI 3616 GCAGGT ACCTGC(4/8)
ClaI 1725 ATCGAT AT^CGAT
DraI 2673 TTTAAA TTT^AAA
DraII 3535 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 1945 GACAATTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2599 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3474 CCTAGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3535 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1114 ATGCAT ATGCA^T
HindIII 2453 AAGCTT A^AGCTT
MfeI 1947 CAATTG C^AATTG
MluI 2566 ACGCGT A^CGCGT
MunI 1947 CAATTG C^AATTG
NsiI 1114 ATGCAT ATGCA^T
SacI 335 GAGCTC GAGCT^C
SmaI 1164 CCCGGG CCC^GGG
SpeI 1263 ACTAGT A^CTAGT
SspI 377 AATATT AAT^ATT
XmaCI 1164 CCCGGG C^CCGGG
XmaI 1164 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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Acc65I 257 GGTACC G^GTACC
550 GGTACC
AccI 688 GTAGAC GT^[AC][GT]AC
1465 GTCTAC
AccII 804 CGCG CG^CG
2567 CGCG
Asp718I 257 GGTACC G^GTACC
550 GGTACC
BcgI 1717 GCAGTGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4452 GCAGAAATCTCG
BpmI 1334 CTGGAG CTGGAG(16/14)
4016 CTGGAG
BsaBI 795 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4110 GATAGGCATC
BspLU11I 2603 ACATGT A^CATGT
3603 ACATGT
BstUI 804 CGCG CG^CG
2567 CGCG
DraIII 1793 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3505 CACCCAGTG
FauI 2342 CCCGC CCCGC(4/6)
805 GCGGG
HgaI 3556 GACGC GACGC(5/10)
3030 GCGTC
KpnI 257 GGTACC GGTAC^C
550 GGTACC
MamI 795 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4110 GATAGGCATC
MvnI 804 CGCG CG^CG
2567 CGCG
PstI 2864 CTGCAG CTGCA^G
3262 CTGCAG
StuI 1856 AGGCCT AGG^CCT
3406 AGGCCT
XcmI 1871 CCAACCAAATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4013 CCACTGGAGAGGTGG
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# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
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AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BciVI BcnI BfaI
BmyI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BsrDI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EarI EcoO65I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI