Restriction map for
pF1KB3020
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AgeI Aor51HI ApoI AscI
AseI AsnI AspI AviII AvrII
BalI BbeI BbrPI BciVI BfaI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrGI BssHII
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI DraIII EarI Eco47III
EcoRI EcoRV EcoT22I FseI FspI
HindIII HpaI KasI Ksp632I KspI
MaeI MfeI MluI MluNI MroI
MscI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tsp509I Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 492 GGTACC G^GTACC
AcyI 1587 GACGCC G[GA]^CG[CT]C
AflIII 1817 ACATGT A^C[GA][CT]GT
AhdI 1186 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1303 GTGCAC G^TGCAC
ApaLI 1303 GTGCAC G^TGCAC
Asp700I 1257 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 492 GGTACC G^GTACC
AspEI 1186 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1530 GGATCC G^GATCC
BbvCI 1807 CCTCAGC CCTCAGC(-5/-2)
BcgI 596 GCAATGGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1275 TGATCA T^GATCA
BsaBI 1096 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1587 GACGCC G[GA]^CG[CT]C
BsaI 1361 GAGACC GGTCTC(1/5)
BsiEI 413 CGGCCG CG[GA][CT]^CG
BspLU11I 1817 ACATGT A^CATGT
BssSI 928 CACGAG CACGAG(-5/-1)
BstEII 1558 GGTGACC G^GT[ACGT]ACC
BstPI 1558 GGTGACC G^GT[ACGT]ACC
DrdI 1627 GACATGGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 413 CGGCCG C^GGCCG
Eam1105I 1186 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 413 CGGCCG C^GGCCG
Eco52I 413 CGGCCG C^GGCCG
EcoNI 855 CCTGAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1558 GGTGACC G^GT[ACGT]ACC
FbaI 1275 TGATCA T^GATCA
Hin1I 1587 GACGCC G[GA]^CG[CT]C
HincII 1891 GTTGAC GT[CT]^[GA]AC
HindII 1891 GTTGAC GT[CT]^[GA]AC
KpnI 492 GGTACC GGTAC^C
MamI 1096 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1109 TTAA T^TAA
MspA1I 958 CAGCTG C[AC]G^C[GT]G
NaeI 607 GCCGGC GCC^GGC
NgoMIV 607 GCCGGC G^CCGGC
PaeR7I 1828 CTCGAG C^TCGAG
PshAI 241 GACGGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 958 CAGCTG CAG^CTG
SacI 1542 GAGCTC GAGCT^C
SexAI 50 ACCAGGT A^CC[AT]GGT
SmlI 1828 CTCGAG C^T[CT][GA]AG
SphI 614 GCATGC GCATG^C
StuI 1270 AGGCCT AGG^CCT
Tru9I 1109 TTAA T^TAA
XhoI 1828 CTCGAG C^TCGAG
XmnI 1257 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AlwNI 484 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
1491 CAGTATCTG
AocI 855 CCTGAGG CC^T[ACGT]AGG
1885 CCTGAGG
BsgI 79 CTGCAC GTGCAG(16/14)
650 GTGCAG
BsmAI 1361 GAGAC GTCTC(1/5)
1821 GTCTC
BspHI 407 TCATGA T^CATGA
1389 TCATGA
BstF5I 764 CATCC GGATG(2/0)
1731 CATCC
Bsu36I 855 CCTGAGG CC^T[ACGT]AGG
1885 CCTGAGG
EaeI 176 TGGCCG [CT]^GGCC[GA]
413 CGGCCG
Eco81I 855 CCTGAGG CC^T[ACGT]AGG
1885 CCTGAGG
FokI 764 CATCC GGATG(9/13)
1731 CATCC
HaeII 113 GGCGCT [GA]GCGC^[CT]
211 AGCGCC
MaeII 125 ACGT A^CGT
479 ACGT
MslI 69 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
375 CATCGTGGTG
NcoI 707 CCATGG C^CATGG
1904 CCATGG
PstI 183 CTGCAG CTGCA^G
1488 CTGCAG
RcaI 407 TCATGA T^CATGA
1389 TCATGA
ScaI 191 AGTACT AGT^ACT
1424 AGTACT
SfcI 183 CTGCAG C^T[GA][CT]AG
1488 CTGCAG
Tsp45I 946 GTCAC ^GT[GC]AC
1559 GTGAC
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BglI
BglII BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TseI TspRI
TthHB8I XcmI XhoII