Restriction map for
pF1KB3016
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# NO CUT ENZYMES ( 118 enzymes) #
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Acc65I AccIII AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BbrPI BcgI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspMI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoT22I FbaI FseI FspI
HgaI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MroI
MspA1I MunI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI PvuII RsrII SacII SalI
SbfI SexAI SfiI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 2215 GACGTC GACGT^C
AccI 2035 GTCTAC GT^[AC][GT]AC
AccII 21 CGCG CG^CG
AclI 1914 AACGTT AA^CGTT
AflIII 1486 ACATGT A^C[GA][CT]GT
AlwNI 887 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1700 GAGCTC G[AT]GC[AT]^C
BbeI 30 GGCGCC GGCGC^C
BbsI 1567 GTCTTC GAAGAC(2/6)
BbvCI 1964 CCTCAGC CCTCAGC(-5/-2)
BciVI 391 GGATAC GTATCC(6/5)
BcnI 5 CCCGG CC^[GC]GG
BfaI 1286 CTAG C^TAG
BpuAI 1567 GTCTTC GAAGAC(2/6)
BsgI 1490 GTGCAG GTGCAG(16/14)
BsiHKAI 1700 GAGCTC G[AT]GC[AT]^C
BsmI 658 GCATTC GAATGC(1/-1)
BspLU11I 1486 ACATGT A^CATGT
BsrDI 1546 GCAATG GCAATG(2/0)
BssSI 740 CACGAG CACGAG(-5/-1)
BstEII 2022 GGTTACC G^GT[ACGT]ACC
BstPI 2022 GGTTACC G^GT[ACGT]ACC
BstUI 21 CGCG CG^CG
BstXI 1705 CCAGCTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 31 GCGC GCG^C
DraI 609 TTTAAA TTT^AAA
DsaI 788 CCATGG C^C[GA][CT]GG
EcoO65I 2022 GGTTACC G^GT[ACGT]ACC
HaeII 30 GGCGCC [GA]GCGC^[CT]
HapII 6 CCGG C^CGG
HhaI 31 GCGC GCG^C
HinP1I 31 GCGC G^CGC
HindIII 1795 AAGCTT A^AGCTT
HpaII 6 CCGG C^CGG
KasI 30 GGCGCC G^GCGCC
MaeI 1286 CTAG C^TAG
MslI 799 CATTTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 6 CCGG C^CGG
MvnI 21 CGCG CG^CG
NarI 30 GGCGCC GG^CGCC
NciI 5 CCCGG CC^[GC]GG
NcoI 788 CCATGG C^CATGG
NspI 1486 ACATGT [GA]CATG^[CT]
PflMI 1000 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 889 GAGTC GAGTC(4/5)
PpuMI 2299 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1914 AACGTT AA^CGTT
SacI 1700 GAGCTC GAGCT^C
SapI 76 GAAGAGC GCTCTTC(1/4)
ScaI 1851 AGTACT AGT^ACT
SfoI 30 GGCGCC GGC^GCC
SmlI 2281 CTTGAG C^T[CT][GA]AG
SpeI 1285 ACTAGT A^CTAGT
Van91I 1000 CCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1118 CCAGATTAGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 30 GGCGCC G[GA]^CG[CT]C
2215 GACGTC
AfaI 1593 GTAC GT^AC
1852 GTAC
BalI 840 TGGCCA TGG^CCA
1428 TGGCCA
BamHI 379 GGATCC G^GATCC
1356 GGATCC
BanI 30 GGCGCC G^G[CT][GA]CC
1715 GGTGCC
BanII 1700 GAGCTC G[GA]GC[CT]^C
2270 GGGCTC
BglI 33 GCCAAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1184 GCCGAGCTGGC
BglII 1524 AGATCT A^GATCT
1900 AGATCT
BmyI 1700 GAGCTC G[AGT]GC[ACT]^C
2270 GGGCTC
BsaHI 30 GGCGCC G[GA]^CG[CT]C
2215 GACGTC
BseRI 46 GAGGAG GAGGAG(10/8)
981 GAGGAG
Bsp1286I 1700 GAGCTC G[AGT]GC[ACT]^C
2270 GGGCTC
BspHI 1974 TCATGA T^CATGA
2010 TCATGA
BsrBI 55 GAGCGG CCGCTC(-3/-3)
79 GAGCGG
DraII 1836 AGGCCCT [GA]G^G[ACGT]CC[CT]
2299 AGGACCC
EarI 76 GAAGAG CTCTTC(1/4)
112 GAAGAG
EcoO109I 1836 AGGCCCT [GA]G^G[ACGT]CC[CT]
2299 AGGACCC
EcoRV 547 GATATC GAT^ATC
793 GATATC
EcoT14I 34 CCAAGG C^C[AT][AT]GG
788 CCATGG
Hin1I 30 GGCGCC G[GA]^CG[CT]C
2215 GACGTC
Ksp632I 76 GAAGAG CTCTTC(1/4)
112 GAAGAG
MluNI 840 TGGCCA TGG^CCA
1428 TGGCCA
MscI 840 TGGCCA TGG^CCA
1428 TGGCCA
RcaI 1974 TCATGA T^CATGA
2010 TCATGA
RsaI 1593 GTAC GT^AC
1852 GTAC
StyI 34 CCAAGG C^C[AT][AT]GG
788 CCATGG
TfiI 1067 GAATC G^A[AT]TC
1731 GATTC
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BbvI BpmI BsaI BsaJI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
FauI Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII