Restriction map for
pF1KB3015
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# NO CUT ENZYMES ( 121 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsoBI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI BstAPI BstEII
BstPI Bsu36I CelII ClaI DraI
DraII DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HgaI KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI SapI
SbfI SfiI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I StuI StyI
SwaI Van91I XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 1063 GACGTC GACGT^C
AccI 1742 GTATAC GT^[AC][GT]AC
AcyI 1063 GACGTC G[GA]^CG[CT]C
AgeI 143 ACCGGT A^CCGGT
AlwNI 370 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1715 AGCGCT AGC^GCT
AspI 709 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1259 TGGCCA TGG^CCA
BanI 1120 GGCACC G^G[CT][GA]CC
BanII 1332 GAGCCC G[GA]GC[CT]^C
BciVI 1670 GTATCC GTATCC(6/5)
BcnI 801 CCCGG CC^[GC]GG
BglII 1280 AGATCT A^GATCT
BsaHI 1063 GACGTC G[GA]^CG[CT]C
BsaWI 143 ACCGGT [AT]^CCGG[AT]
BsgI 1709 CTGCAC GTGCAG(16/14)
Bsp1407I 342 TGTACA T^GTACA
BsrDI 853 GCAATG GCAATG(2/0)
BsrGI 342 TGTACA T^GTACA
Bst1107I 1742 GTATAC GTA^TAC
BstBI 1682 TTCGAA TT^CGAA
BstZ17I 1742 GTATAC GTA^TAC
CpoI 145 CGGTCCG CG^G[AT]CCG
DrdI 615 GACATATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1306 CCGTGG C^C[GA][CT]GG
EaeI 1259 TGGCCA [CT]^GGCC[GA]
Eco47III 1715 AGCGCT AGC^GCT
EcoNI 1048 CCTTTGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 1715 AGCGCT [GA]GCGC^[CT]
Hin1I 1063 GACGTC G[GA]^CG[CT]C
HindIII 1645 AAGCTT A^AGCTT
HpaI 1035 GTTAAC GTT^AAC
MaeII 1064 ACGT A^CGT
MluNI 1259 TGGCCA TGG^CCA
MscI 1259 TGGCCA TGG^CCA
NciI 801 CCCGG CC^[GC]GG
NspI 160 GCATGC [GA]CATG^[CT]
NspV 1682 TTCGAA TT^CGAA
PflFI 709 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 143 ACCGGT A^CCGGT
RsrII 145 CGGTCCG CG^G[AT]CCG
SexAI 1341 ACCAGGT A^CC[AT]GGT
SfuI 1682 TTCGAA TT^CGAA
SmlI 180 CTCAAG C^T[CT][GA]AG
SphI 160 GCATGC GCATG^C
SspBI 342 TGTACA T^GTACA
SspI 349 AATATT AAT^ATT
Tth111I 709 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 492 TCTAGA T^CTAGA
XcmI 690 CCAAAAAGCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 146 GGTCC G^G[AT]CC
480 GGACC
BsmBI 1502 GAGACG CGTCTC(1/5)
1513 GAGACG
BsmI 415 GAATGC GAATGC(1/-1)
784 GAATGC
BsrFI 143 ACCGGT [GA]^CCGG[CT]
2021 ACCGGC
BstF5I 309 GGATG GGATG(2/0)
642 GGATG
BstXI 110 CCATCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
195 CCAGGCTGATGG
Cfr10I 143 ACCGGT [GA]^CCGG[CT]
2021 ACCGGC
EarI 221 CTCTTC CTCTTC(1/4)
229 GAAGAG
FokI 309 GGATG GGATG(9/13)
642 GGATG
Ksp632I 221 CTCTTC CTCTTC(1/4)
229 GAAGAG
PstI 401 CTGCAG CTGCA^G
1928 CTGCAG
PvuII 1660 CAGCTG CAG^CTG
1925 CAGCTG
SfaNI 643 GATGC GCATC(5/9)
1144 GCATC
SfcI 401 CTGCAG C^T[GA][CT]AG
1928 CTGCAG
TaqI 1611 TCGA T^CGA
1683 TCGA
TthHB8I 1611 TCGA T^CGA
1683 TCGA
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI BbvI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NdeI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScaI ScrFI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII