Restriction map for
pF1KB3011
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcyI AgeI AhdI AocI
AscI Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BlnI BlpI Bpu1102I BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoT22I
FauI FbaI FseI FspI HapII
HgaI Hin1I HpaII KasI KpnI
KspI MluI MluNI MroI MscI
MspI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 112 GTAGAC GT^[AC][GT]AC
AflII 2068 CTTAAG C^TTAAG
AflIII 641 ACATGT A^C[GA][CT]GT
Alw44I 701 GTGCAC G^TGCAC
AlwNI 1534 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1515 AGCGCT AGC^GCT
ApaI 363 GGGCCC GGGCC^C
ApaLI 701 GTGCAC G^TGCAC
AseI 478 ATTAAT AT^TAAT
AsnI 478 ATTAAT AT^TAAT
AvaI 2020 CTCGAG C^[CT]CG[GA]G
AvaII 253 GGTCC G^G[AT]CC
BfrI 2068 CTTAAG C^TTAAG
BglI 1266 GCCTTGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 188 TACGTG [CT]AC^GT[GA]
BsaBI 1207 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 902 CCATGG C^C[ACGT][ACGT]GG
BsgI 445 CTGCAC GTGCAG(16/14)
BsmI 2144 GCATTC GAATGC(1/-1)
BsoBI 2020 CTCGAG C^[CT]CG[GA]G
BspLU11I 641 ACATGT A^CATGT
BspMI 697 GCAGGT ACCTGC(4/8)
BsrDI 89 GCAATG GCAATG(2/0)
BstEII 2060 GGTCACC G^GT[ACGT]ACC
BstPI 2060 GGTCACC G^GT[ACGT]ACC
CfoI 1516 GCGC GCG^C
DsaI 902 CCATGG C^C[GA][CT]GG
EarI 376 GAAGAG CTCTTC(1/4)
Eco47III 1515 AGCGCT AGC^GCT
EcoO65I 2060 GGTCACC G^GT[ACGT]ACC
EcoRV 1695 GATATC GAT^ATC
EcoT14I 902 CCATGG C^C[AT][AT]GG
HaeII 1515 AGCGCT [GA]GCGC^[CT]
HhaI 1516 GCGC GCG^C
HinP1I 1516 GCGC G^CGC
Ksp632I 376 GAAGAG CTCTTC(1/4)
MamI 1207 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1431 CAATTG C^AATTG
MunI 1431 CAATTG C^AATTG
NcoI 902 CCATGG C^CATGG
PaeR7I 2020 CTCGAG C^TCGAG
PleI 2023 GAGTC GAGTC(4/5)
PshAI 511 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 478 ATTAAT AT^TAAT
PstI 650 CTGCAG CTGCA^G
SacI 1148 GAGCTC GAGCT^C
SapI 376 GAAGAGC GCTCTTC(1/4)
SpeI 1876 ACTAGT A^CTAGT
SphI 381 GCATGC GCATG^C
StyI 902 CCATGG C^C[AT][AT]GG
XcmI 1785 CCAGTTTGTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2020 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 25 enzymes) #
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Asp700I 736 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1975 GAAGGATTTC
AspHI 701 GTGCAC G[AT]GC[AT]^C
1148 GAGCTC
BamHI 534 GGATCC G^GATCC
1233 GGATCC
BsiHKAI 701 GTGCAC G[AT]GC[AT]^C
1148 GAGCTC
BsiYI 366 CCCTTTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
902 CCATGGATCGG
BslI 366 CCCTTTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
902 CCATGGATCGG
BsmAI 334 GAGAC GTCTC(1/5)
20 GTCTC
Bsp1407I 750 TGTACA T^GTACA
1104 TGTACA
BsrGI 750 TGTACA T^GTACA
1104 TGTACA
Cac8I 381 GCATGC GC[ACGT]^[ACGT]GC
652 GCAGGC
DraII 362 AGGGCCC [GA]G^G[ACGT]CC[CT]
363 GGGCCCT
EcoO109I 362 AGGGCCC [GA]G^G[ACGT]CC[CT]
363 GGGCCCT
HincII 912 GTTAAC GT[CT]^[GA]AC
1390 GTTAAC
HindII 912 GTTAAC GT[CT]^[GA]AC
1390 GTTAAC
HindIII 1411 AAGCTT A^AGCTT
1951 AAGCTT
HpaI 912 GTTAAC GTT^AAC
1390 GTTAAC
HphI 508 GGTGA GGTGA(8/7)
2062 TCACC
MaeII 189 ACGT A^CGT
600 ACGT
MslI 636 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1396 CATTACTATG
SfcI 110 CTGTAG C^T[GA][CT]AG
650 CTGCAG
SspBI 750 TGTACA T^GTACA
1104 TGTACA
SspI 1199 AATATT AAT^ATT
1301 AATATT
TaqI 858 TCGA T^CGA
2021 TCGA
TthHB8I 858 TCGA T^CGA
2021 TCGA
XmnI 736 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1975 GAAGGATTTC
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BanII BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsmFI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII