################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## Acc65I AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AvrII BbrPI BcgI BclI BglI BlnI BsaAI BseAI BsmBI BspDI BspEI BspMI BssHII ClaI DraI Eam1105I Eco47III EcoNI FbaI FseI KpnI KspI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI PacI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI SacII SalI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI Sse8387I SspI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1784 GACGTC GACGT^C AflII 3184 CTTAAG C^TTAAG ApaI 733 GGGCCC GGGCC^C AseI 1375 ATTAAT AT^TAAT AsnI 1375 ATTAAT AT^TAAT AspI 666 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4828 TGCGCA TGC^GCA BamHI 3598 GGATCC G^GATCC BbeI 1817 GGCGCC GGCGC^C BbvCI 2308 GCTGAGG CCTCAGC(-5/-2) BciVI 3691 GTATCC GTATCC(6/5) BfrI 3184 CTTAAG C^TTAAG BsaBI 4134 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1266 TCCGGT [AT]^CCGG[AT] BsiWI 3711 CGTACG C^GTACG BsmFI 3930 GGGAC GGGAC(10/14) BsrFI 136 ACCGGC [GA]^CCGG[CT] Bst1107I 1080 GTATAC GTA^TAC BstBI 2342 TTCGAA TT^CGAA BstZ17I 1080 GTATAC GTA^TAC Cfr10I 136 ACCGGC [GA]^CCGG[CT] CpoI 3229 CGGACCG CG^G[AT]CCG DraIII 2905 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT22I 3421 ATGCAT ATGCA^T FauI 4 GCGGG CCCGC(4/6) FspI 4828 TGCGCA TGC^GCA HincII 862 GTTAAC GT[CT]^[GA]AC HindII 862 GTTAAC GT[CT]^[GA]AC HindIII 198 AAGCTT A^AGCTT HpaI 862 GTTAAC GTT^AAC KasI 1817 GGCGCC G^GCGCC MamI 4134 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 1817 GGCGCC GG^CGCC NsiI 3421 ATGCAT ATGCA^T NspV 2342 TTCGAA TT^CGAA PflFI 666 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1375 ATTAAT AT^TAAT RsrII 3229 CGGACCG CG^G[AT]CCG SapI 4263 GAAGAGC GCTCTTC(1/4) SfoI 1817 GGCGCC GGC^GCC SfuI 2342 TTCGAA TT^CGAA SpeI 1164 ACTAGT A^CTAGT StuI 2480 AGGCCT AGG^CCT Tth111I 666 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 1080 GTATAC GT^[AC][GT]AC 4867 GTAGAC AccII 27 CGCG CG^CG 3779 CGCG AocI 580 CCTGAGG CC^T[ACGT]AGG 1812 CCTAAGG BalI 3818 TGGCCA TGG^CCA 4652 TGGCCA BglII 558 AGATCT A^GATCT 4356 AGATCT BsiEI 108 CGGCCG CG[GA][CT]^CG 154 CGGCCG Bsp1407I 1655 TGTACA T^GTACA 1959 TGTACA BsrGI 1655 TGTACA T^GTACA 1959 TGTACA BssSI 1837 CACGAG CACGAG(-5/-1) 1700 CTCGTG BstUI 27 CGCG CG^CG 3779 CGCG Bsu36I 580 CCTGAGG CC^T[ACGT]AGG 1812 CCTAAGG DraII 3731 AGGCCCT [GA]G^G[ACGT]CC[CT] 4849 AGGCCCT DrdI 2218 GACACTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3672 GACTATTCTGTC EagI 108 CGGCCG C^GGCCG 154 CGGCCG EclXI 108 CGGCCG C^GGCCG 154 CGGCCG Eco52I 108 CGGCCG C^GGCCG 154 CGGCCG Eco81I 580 CCTGAGG CC^T[ACGT]AGG 1812 CCTAAGG EcoO109I 3731 AGGCCCT [GA]G^G[ACGT]CC[CT] 4849 AGGCCCT EcoRI 491 GAATTC G^AATTC 3192 GAATTC HaeII 224 AGCGCC [GA]GCGC^[CT] 1817 GGCGCC MluNI 3818 TGGCCA TGG^CCA 4652 TGGCCA MscI 3818 TGGCCA TGG^CCA 4652 TGGCCA MvnI 27 CGCG CG^CG 3779 CGCG NcoI 1898 CCATGG C^CATGG 3371 CCATGG SacI 2839 GAGCTC GAGCT^C 3532 GAGCTC SphI 2032 GCATGC GCATG^C 4197 GCATGC SspBI 1655 TGTACA T^GTACA 1959 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 121 enzymes) # ######################################### AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmI BsoBI Bsp1286I BspHI BspLU11I BsrBI BsrDI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoO65I EcoRII EcoRV EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PaeR7I PflMI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoI XhoII XmnI