Restriction map for pF1KB0016
##################################
# NO CUT ENZYMES (   66 enzymes) #
##################################

Acc65I      AccIII      AclI        AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AvrII       BbrPI       BcgI        BclI        
BglI        BlnI        BsaAI       BseAI       BsmBI       
BspDI       BspEI       BspMI       BssHII      ClaI        
DraI        Eam1105I    Eco47III    EcoNI       FbaI        
FseI        KpnI        KspI        MfeI        MluI        
MroI        MunI        NaeI        NgoMIV      NheI        
NotI        NruI        PacI        PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       Psp1406I    
PvuI        SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SgrAI       SmaI        SnaBI       
Sse8387I    SspI        SwaI        XbaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AatII        1784  GACGTC               GACGT^C
AflII        3184  CTTAAG               C^TTAAG
ApaI          733  GGGCCC               GGGCC^C
AseI         1375  ATTAAT               AT^TAAT
AsnI         1375  ATTAAT               AT^TAAT
AspI          666  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        4828  TGCGCA               TGC^GCA
BamHI        3598  GGATCC               G^GATCC
BbeI         1817  GGCGCC               GGCGC^C
BbvCI        2308  GCTGAGG              CCTCAGC(-5/-2)
BciVI        3691  GTATCC               GTATCC(6/5)
BfrI         3184  CTTAAG               C^TTAAG
BsaBI        4134  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1266  TCCGGT               [AT]^CCGG[AT]
BsiWI        3711  CGTACG               C^GTACG
BsmFI        3930  GGGAC                GGGAC(10/14)
BsrFI         136  ACCGGC               [GA]^CCGG[CT]
Bst1107I     1080  GTATAC               GTA^TAC
BstBI        2342  TTCGAA               TT^CGAA
BstZ17I      1080  GTATAC               GTA^TAC
Cfr10I        136  ACCGGC               [GA]^CCGG[CT]
CpoI         3229  CGGACCG              CG^G[AT]CCG
DraIII       2905  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I      3421  ATGCAT               ATGCA^T
FauI            4  GCGGG                CCCGC(4/6)
FspI         4828  TGCGCA               TGC^GCA
HincII        862  GTTAAC               GT[CT]^[GA]AC
HindII        862  GTTAAC               GT[CT]^[GA]AC
HindIII       198  AAGCTT               A^AGCTT
HpaI          862  GTTAAC               GTT^AAC
KasI         1817  GGCGCC               G^GCGCC
MamI         4134  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI         1817  GGCGCC               GG^CGCC
NsiI         3421  ATGCAT               ATGCA^T
NspV         2342  TTCGAA               TT^CGAA
PflFI         666  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI        1375  ATTAAT               AT^TAAT
RsrII        3229  CGGACCG              CG^G[AT]CCG
SapI         4263  GAAGAGC              GCTCTTC(1/4)
SfoI         1817  GGCGCC               GGC^GCC
SfuI         2342  TTCGAA               TT^CGAA
SpeI         1164  ACTAGT               A^CTAGT
StuI         2480  AGGCCT               AGG^CCT
Tth111I       666  GACTCAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         1080  GTATAC               GT^[AC][GT]AC
             4867  GTAGAC              
AccII          27  CGCG                 CG^CG
             3779  CGCG                
AocI          580  CCTGAGG              CC^T[ACGT]AGG
             1812  CCTAAGG             
BalI         3818  TGGCCA               TGG^CCA
             4652  TGGCCA              
BglII         558  AGATCT               A^GATCT
             4356  AGATCT              
BsiEI         108  CGGCCG               CG[GA][CT]^CG
              154  CGGCCG              
Bsp1407I     1655  TGTACA               T^GTACA
             1959  TGTACA              
BsrGI        1655  TGTACA               T^GTACA
             1959  TGTACA              
BssSI        1837  CACGAG               CACGAG(-5/-1)
             1700  CTCGTG              
BstUI          27  CGCG                 CG^CG
             3779  CGCG                
Bsu36I        580  CCTGAGG              CC^T[ACGT]AGG
             1812  CCTAAGG             
DraII        3731  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             4849  AGGCCCT             
DrdI         2218  GACACTTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3672  GACTATTCTGTC        
EagI          108  CGGCCG               C^GGCCG
              154  CGGCCG              
EclXI         108  CGGCCG               C^GGCCG
              154  CGGCCG              
Eco52I        108  CGGCCG               C^GGCCG
              154  CGGCCG              
Eco81I        580  CCTGAGG              CC^T[ACGT]AGG
             1812  CCTAAGG             
EcoO109I     3731  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             4849  AGGCCCT             
EcoRI         491  GAATTC               G^AATTC
             3192  GAATTC              
HaeII         224  AGCGCC               [GA]GCGC^[CT]
             1817  GGCGCC              
MluNI        3818  TGGCCA               TGG^CCA
             4652  TGGCCA              
MscI         3818  TGGCCA               TGG^CCA
             4652  TGGCCA              
MvnI           27  CGCG                 CG^CG
             3779  CGCG                
NcoI         1898  CCATGG               C^CATGG
             3371  CCATGG              
SacI         2839  GAGCTC               GAGCT^C
             3532  GAGCTC              
SphI         2032  GCATGC               GCATG^C
             4197  GCATGC              
SspBI        1655  TGTACA               T^GTACA
             1959  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (  121 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AflIII      
AluI        AlwI        AlwNI       ApoI        Asp700I     
AspHI       AvaI        AvaII       BanI        BanII       
BbsI        BbvI        BcnI        BfaI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmI        BsoBI       
Bsp1286I    BspHI       BspLU11I    BsrBI       BsrDI       
BsrI        BssKI       BstAPI      BstEII      BstF5I      
BstNI       BstPI       BstXI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        EcoO65I     EcoRII      
EcoRV       EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PaeR7I      
PflMI       PleI        PstI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     Van91I      XcmI        XhoI        XhoII       
XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com