Restriction map for
pF1KB0016
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI AvrII BbrPI BcgI BclI
BglI BlnI BsaAI BseAI BsmBI
BspDI BspEI BspMI BssHII ClaI
DraI Eam1105I Eco47III EcoNI FbaI
FseI KpnI KspI MfeI MluI
MroI MunI NaeI NgoMIV NheI
NotI NruI PacI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI SacII SalI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
Sse8387I SspI SwaI XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AatII 1784 GACGTC GACGT^C
AflII 3184 CTTAAG C^TTAAG
ApaI 733 GGGCCC GGGCC^C
AseI 1375 ATTAAT AT^TAAT
AsnI 1375 ATTAAT AT^TAAT
AspI 666 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4828 TGCGCA TGC^GCA
BamHI 3598 GGATCC G^GATCC
BbeI 1817 GGCGCC GGCGC^C
BbvCI 2308 GCTGAGG CCTCAGC(-5/-2)
BciVI 3691 GTATCC GTATCC(6/5)
BfrI 3184 CTTAAG C^TTAAG
BsaBI 4134 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1266 TCCGGT [AT]^CCGG[AT]
BsiWI 3711 CGTACG C^GTACG
BsmFI 3930 GGGAC GGGAC(10/14)
BsrFI 136 ACCGGC [GA]^CCGG[CT]
Bst1107I 1080 GTATAC GTA^TAC
BstBI 2342 TTCGAA TT^CGAA
BstZ17I 1080 GTATAC GTA^TAC
Cfr10I 136 ACCGGC [GA]^CCGG[CT]
CpoI 3229 CGGACCG CG^G[AT]CCG
DraIII 2905 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT22I 3421 ATGCAT ATGCA^T
FauI 4 GCGGG CCCGC(4/6)
FspI 4828 TGCGCA TGC^GCA
HincII 862 GTTAAC GT[CT]^[GA]AC
HindII 862 GTTAAC GT[CT]^[GA]AC
HindIII 198 AAGCTT A^AGCTT
HpaI 862 GTTAAC GTT^AAC
KasI 1817 GGCGCC G^GCGCC
MamI 4134 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1817 GGCGCC GG^CGCC
NsiI 3421 ATGCAT ATGCA^T
NspV 2342 TTCGAA TT^CGAA
PflFI 666 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1375 ATTAAT AT^TAAT
RsrII 3229 CGGACCG CG^G[AT]CCG
SapI 4263 GAAGAGC GCTCTTC(1/4)
SfoI 1817 GGCGCC GGC^GCC
SfuI 2342 TTCGAA TT^CGAA
SpeI 1164 ACTAGT A^CTAGT
StuI 2480 AGGCCT AGG^CCT
Tth111I 666 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1080 GTATAC GT^[AC][GT]AC
4867 GTAGAC
AccII 27 CGCG CG^CG
3779 CGCG
AocI 580 CCTGAGG CC^T[ACGT]AGG
1812 CCTAAGG
BalI 3818 TGGCCA TGG^CCA
4652 TGGCCA
BglII 558 AGATCT A^GATCT
4356 AGATCT
BsiEI 108 CGGCCG CG[GA][CT]^CG
154 CGGCCG
Bsp1407I 1655 TGTACA T^GTACA
1959 TGTACA
BsrGI 1655 TGTACA T^GTACA
1959 TGTACA
BssSI 1837 CACGAG CACGAG(-5/-1)
1700 CTCGTG
BstUI 27 CGCG CG^CG
3779 CGCG
Bsu36I 580 CCTGAGG CC^T[ACGT]AGG
1812 CCTAAGG
DraII 3731 AGGCCCT [GA]G^G[ACGT]CC[CT]
4849 AGGCCCT
DrdI 2218 GACACTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3672 GACTATTCTGTC
EagI 108 CGGCCG C^GGCCG
154 CGGCCG
EclXI 108 CGGCCG C^GGCCG
154 CGGCCG
Eco52I 108 CGGCCG C^GGCCG
154 CGGCCG
Eco81I 580 CCTGAGG CC^T[ACGT]AGG
1812 CCTAAGG
EcoO109I 3731 AGGCCCT [GA]G^G[ACGT]CC[CT]
4849 AGGCCCT
EcoRI 491 GAATTC G^AATTC
3192 GAATTC
HaeII 224 AGCGCC [GA]GCGC^[CT]
1817 GGCGCC
MluNI 3818 TGGCCA TGG^CCA
4652 TGGCCA
MscI 3818 TGGCCA TGG^CCA
4652 TGGCCA
MvnI 27 CGCG CG^CG
3779 CGCG
NcoI 1898 CCATGG C^CATGG
3371 CCATGG
SacI 2839 GAGCTC GAGCT^C
3532 GAGCTC
SphI 2032 GCATGC GCATG^C
4197 GCATGC
SspBI 1655 TGTACA T^GTACA
1959 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmI BsoBI
Bsp1286I BspHI BspLU11I BsrBI BsrDI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DsaI EaeI EarI EcoO65I EcoRII
EcoRV EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PaeR7I
PflMI PleI PstI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmnI