################################## # NO CUT ENZYMES ( 48 enzymes) # ################################## AclI AflII AgeI Aor51HI AseI AsnI AvrII BciVI BfrI BlnI BspDI BspLU11I Bst1107I BstBI BstZ17I ClaI CpoI DraI Eco47III EcoRI EcoRV EcoT22I FseI HpaI KspI MfeI MluI MunI NheI NotI NruI NsiI NspV PacI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SfuI SgrAI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 3896 GGTACC G^GTACC ApaI 6058 GGGCCC GGGCC^C AscI 6088 GGCGCGCC GG^CGCGCC Asp718I 3896 GGTACC G^GTACC AviII 2645 TGCGCA TGC^GCA BglII 521 AGATCT A^GATCT BsaBI 5634 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiWI 530 CGTACG C^GTACG BsmI 4314 GAATGC GAATGC(1/-1) BspHI 5345 TCATGA T^CATGA BsrDI 153 CATTGC GCAATG(2/0) BssHII 6089 GCGCGC G^CGCGC EagI 3748 CGGCCG C^GGCCG EclXI 3748 CGGCCG C^GGCCG Eco52I 3748 CGGCCG C^GGCCG FspI 2645 TGCGCA TGC^GCA HindIII 1390 AAGCTT A^AGCTT KpnI 3896 GGTACC GGTAC^C MamI 5634 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 5863 GCCGGC GCC^GGC NdeI 364 CATATG CA^TATG NgoMIV 5863 GCCGGC G^CCGGC PaeR7I 5503 CTCGAG C^TCGAG PshAI 2805 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 5345 TCATGA T^CATGA SalI 4228 GTCGAC G^TCGAC ScaI 1856 AGTACT AGT^ACT SphI 2630 GCATGC GCATG^C XhoI 5503 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 4228 GTCGAC GT^[AC][GT]AC 5104 GTAGAC AcsI 892 AAATTC [GA]^AATT[CT] 1054 GAATTT AhdI 1306 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2383 GACTCCAAGTC Alw44I 253 GTGCAC G^TGCAC 5020 GTGCAC AocI 298 CCTCAGG CC^T[ACGT]AGG 3301 CCTGAGG ApaLI 253 GTGCAC G^TGCAC 5020 GTGCAC ApoI 892 AAATTC [GA]^AATT[CT] 1054 GAATTT Asp700I 3601 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4815 GAACGACTTC AspEI 1306 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2383 GACTCCAAGTC AspI 1720 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3218 GACAGGGTC BamHI 5906 GGATCC G^GATCC 6423 GGATCC BcgI 2127 CGAAAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 16 GCAGGCAGCTCG BclI 2237 TGATCA T^GATCA 5639 TGATCA BsiEI 2424 CGACCG CG[GA][CT]^CG 3748 CGGCCG BstEII 1374 GGTGACC G^GT[ACGT]ACC 3543 GGTCACC BstPI 1374 GGTGACC G^GT[ACGT]ACC 3543 GGTCACC Bsu36I 298 CCTCAGG CC^T[ACGT]AGG 3301 CCTGAGG DrdI 2596 GACGAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3769 GACAGCAGGGTC Eam1105I 1306 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2383 GACTCCAAGTC Eco81I 298 CCTCAGG CC^T[ACGT]AGG 3301 CCTGAGG EcoO65I 1374 GGTGACC G^GT[ACGT]ACC 3543 GGTCACC FbaI 2237 TGATCA T^GATCA 5639 TGATCA PflFI 1720 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3218 GACAGGGTC SapI 1042 GAAGAGC GCTCTTC(1/4) 2173 GAAGAGC SbfI 2735 CCTGCAGG CCTGCA^GG 2841 CCTGCAGG SexAI 4757 ACCTGGT A^CC[AT]GGT 4913 ACCAGGT Sse8387I 2735 CCTGCAGG CCTGCA^GG 2841 CCTGCAGG Tsp509I 893 AATT ^AATT 1055 AATT Tth111I 1720 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC 3218 GACAGGGTC XmnI 3601 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4815 GAACGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 151 enzymes) # ######################################### AatII AccII AccIII AciI AcyI AfaI AflIII AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspEI BspMI BsrBI BsrFI BsrGI BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SfoI SmaI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI