Restriction map for
pF1KB0013
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# NO CUT ENZYMES ( 48 enzymes) #
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AclI AflII AgeI Aor51HI AseI
AsnI AvrII BciVI BfrI BlnI
BspDI BspLU11I Bst1107I BstBI BstZ17I
ClaI CpoI DraI Eco47III EcoRI
EcoRV EcoT22I FseI HpaI KspI
MfeI MluI MunI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI
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# ONE CUT ENZYMES ( 29 enzymes) #
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Acc65I 3896 GGTACC G^GTACC
ApaI 6058 GGGCCC GGGCC^C
AscI 6088 GGCGCGCC GG^CGCGCC
Asp718I 3896 GGTACC G^GTACC
AviII 2645 TGCGCA TGC^GCA
BglII 521 AGATCT A^GATCT
BsaBI 5634 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 530 CGTACG C^GTACG
BsmI 4314 GAATGC GAATGC(1/-1)
BspHI 5345 TCATGA T^CATGA
BsrDI 153 CATTGC GCAATG(2/0)
BssHII 6089 GCGCGC G^CGCGC
EagI 3748 CGGCCG C^GGCCG
EclXI 3748 CGGCCG C^GGCCG
Eco52I 3748 CGGCCG C^GGCCG
FspI 2645 TGCGCA TGC^GCA
HindIII 1390 AAGCTT A^AGCTT
KpnI 3896 GGTACC GGTAC^C
MamI 5634 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 5863 GCCGGC GCC^GGC
NdeI 364 CATATG CA^TATG
NgoMIV 5863 GCCGGC G^CCGGC
PaeR7I 5503 CTCGAG C^TCGAG
PshAI 2805 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 5345 TCATGA T^CATGA
SalI 4228 GTCGAC G^TCGAC
ScaI 1856 AGTACT AGT^ACT
SphI 2630 GCATGC GCATG^C
XhoI 5503 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 4228 GTCGAC GT^[AC][GT]AC
5104 GTAGAC
AcsI 892 AAATTC [GA]^AATT[CT]
1054 GAATTT
AhdI 1306 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2383 GACTCCAAGTC
Alw44I 253 GTGCAC G^TGCAC
5020 GTGCAC
AocI 298 CCTCAGG CC^T[ACGT]AGG
3301 CCTGAGG
ApaLI 253 GTGCAC G^TGCAC
5020 GTGCAC
ApoI 892 AAATTC [GA]^AATT[CT]
1054 GAATTT
Asp700I 3601 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4815 GAACGACTTC
AspEI 1306 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2383 GACTCCAAGTC
AspI 1720 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3218 GACAGGGTC
BamHI 5906 GGATCC G^GATCC
6423 GGATCC
BcgI 2127 CGAAAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
16 GCAGGCAGCTCG
BclI 2237 TGATCA T^GATCA
5639 TGATCA
BsiEI 2424 CGACCG CG[GA][CT]^CG
3748 CGGCCG
BstEII 1374 GGTGACC G^GT[ACGT]ACC
3543 GGTCACC
BstPI 1374 GGTGACC G^GT[ACGT]ACC
3543 GGTCACC
Bsu36I 298 CCTCAGG CC^T[ACGT]AGG
3301 CCTGAGG
DrdI 2596 GACGAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3769 GACAGCAGGGTC
Eam1105I 1306 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2383 GACTCCAAGTC
Eco81I 298 CCTCAGG CC^T[ACGT]AGG
3301 CCTGAGG
EcoO65I 1374 GGTGACC G^GT[ACGT]ACC
3543 GGTCACC
FbaI 2237 TGATCA T^GATCA
5639 TGATCA
PflFI 1720 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3218 GACAGGGTC
SapI 1042 GAAGAGC GCTCTTC(1/4)
2173 GAAGAGC
SbfI 2735 CCTGCAGG CCTGCA^GG
2841 CCTGCAGG
SexAI 4757 ACCTGGT A^CC[AT]GGT
4913 ACCAGGT
Sse8387I 2735 CCTGCAGG CCTGCA^GG
2841 CCTGCAGG
Tsp509I 893 AATT ^AATT
1055 AATT
Tth111I 1720 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
3218 GACAGGGTC
XmnI 3601 GAAACCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4815 GAACGACTTC
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# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AatII AccII AccIII AciI AcyI
AfaI AflIII AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspEI BspMI BsrBI BsrFI
BsrGI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI SfoI SmaI
SmlI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI