Restriction map for
pF1KB0011
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# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflIII AgeI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI BamHI
BbeI BbvCI BcgI BciVI BclI
BglII BlpI Bpu1102I BsaBI BsaHI
BsaI BsiEI BsiHKAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrGI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI Hin1I
HincII HindII HpaI KasI KpnI
MamI MfeI MluI MslI MunI
NarI NdeI NotI NsiI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI XbaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 1142 TCCGGA T^CCGGA
AfaI 157 GTAC GT^AC
AflII 1222 CTTAAG C^TTAAG
AhdI 44 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 44 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1393 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 935 TGCGCA TGC^GCA
AvrII 714 CCTAGG C^CTAGG
BalI 863 TGGCCA TGG^CCA
BbrPI 490 CACGTG CAC^GTG
BbsI 1036 GAAGAC GAAGAC(2/6)
BfrI 1222 CTTAAG C^TTAAG
BlnI 714 CCTAGG C^CTAGG
BpuAI 1036 GAAGAC GAAGAC(2/6)
BseAI 1142 TCCGGA T^CCGGA
BspEI 1142 TCCGGA T^CCGGA
BsrBI 512 CCGCTC CCGCTC(-3/-3)
BsrFI 532 GCCGGC [GA]^CCGG[CT]
BsrI 154 CCAGT ACTGG(1/-1)
BssHII 1308 GCGCGC G^CGCGC
BssSI 574 CTCGTG CACGAG(-5/-1)
BstXI 1112 CCAGCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 532 GCCGGC [GA]^CCGG[CT]
Eam1105I 44 GACCCGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 352 CCTTTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 935 TGCGCA TGC^GCA
HaeII 64 GGCGCT [GA]GCGC^[CT]
HindIII 1357 AAGCTT A^AGCTT
KspI 915 CCGCGG CCGC^GG
MluNI 863 TGGCCA TGG^CCA
MroI 1142 TCCGGA T^CCGGA
MscI 863 TGGCCA TGG^CCA
MseI 1223 TTAA T^TAA
NaeI 532 GCCGGC GCC^GGC
NgoMIV 532 GCCGGC G^CCGGC
NheI 784 GCTAGC G^CTAGC
NruI 260 TCGCGA TCG^CGA
NspI 1370 GCATGT [GA]CATG^[CT]
PaeR7I 413 CTCGAG C^TCGAG
PflFI 1393 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 154 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 490 CACGTG CAC^GTG
PmlI 490 CACGTG CAC^GTG
RsaI 157 GTAC GT^AC
SacII 915 CCGCGG CCGC^GG
SapI 685 GAAGAGC GCTCTTC(1/4)
SfiI 974 GGCCCCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
TaqI 414 TCGA T^CGA
Tru9I 1223 TTAA T^TAA
Tth111I 1393 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 414 TCGA T^CGA
Van91I 154 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 845 CCAGCCTGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 413 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
BanI 186 GGTGCC G^G[CT][GA]CC
814 GGTGCC
BfaI 715 CTAG C^TAG
785 CTAG
BsaAI 490 CACGTG [CT]AC^GT[GA]
1303 TACGTG
BsaWI 1142 TCCGGA [AT]^CCGG[AT]
1382 ACCGGA
BseRI 519 CTCCTC GAGGAG(10/8)
778 GAGGAG
BsgI 665 CTGCAC GTGCAG(16/14)
35 GTGCAG
BsmI 938 GCATTC GAATGC(1/-1)
1076 GCATTC
BspMI 225 GCAGGT ACCTGC(4/8)
279 GCAGGT
BsrDI 872 CATTGC GCAATG(2/0)
1166 GCAATG
BstAPI 307 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
808 GCAGGAGGTGC
BstYI 1145 GGATCT [GA]^GATC[CT]
1158 GGATCT
EarI 685 GAAGAG CTCTTC(1/4)
745 GAAGAG
HgaI 471 GCGTC GACGC(5/10)
960 GCGTC
Ksp632I 685 GAAGAG CTCTTC(1/4)
745 GAAGAG
MaeI 715 CTAG C^TAG
785 CTAG
MaeIII 392 GTCAC ^GT[ACGT]AC
1241 GTGAC
MflI 1145 GGATCT [GA]^GATC[CT]
1158 GGATCT
NcoI 335 CCATGG C^CATGG
659 CCATGG
StuI 717 AGGCCT AGG^CCT
999 AGGCCT
Tsp45I 392 GTCAC ^GT[GC]AC
1241 GTGAC
Tsp509I 255 AATT ^AATT
1064 AATT
XhoII 1145 GGATCT [GA]^GATC[CT]
1158 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AvaI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TfiI TseI TspRI XmaCI
XmaI