Restriction map for
pF1KA1776
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# NO CUT ENZYMES ( 40 enzymes) #
##################################
AclI AflII AseI AsnI AvrII
BciVI BfrI BlnI BsaBI BsiWI
Bsp1407I BsrBI BsrGI Bst1107I BstZ17I
CpoI DraI HpaI MamI MfeI
MluI MunI NdeI NheI NotI
NruI PacI PmeI PshBI Psp1406I
PvuI RsrII SapI SbfI ScaI
SnaBI SpeI Sse8387I SspBI SwaI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2809 GACGTC GACGT^C
AccIII 1938 TCCGGA T^CCGGA
AcsI 1021 GAATTC [GA]^AATT[CT]
AhdI 2926 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 6181 AGCGCT AGC^GCT
ApoI 1021 GAATTC [GA]^AATT[CT]
AscI 260 GGCGCGCC GG^CGCGCC
Asp700I 4470 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2926 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1843 CACGTG CAC^GTG
BclI 7803 TGATCA T^GATCA
BglII 2285 AGATCT A^GATCT
BsaAI 1843 CACGTG [CT]AC^GT[GA]
BseAI 1938 TCCGGA T^CCGGA
BsmBI 4483 GAGACG CGTCTC(1/5)
BspDI 3340 ATCGAT AT^CGAT
BspEI 1938 TCCGGA T^CCGGA
BspHI 6479 TCATGA T^CATGA
BssHII 261 GCGCGC G^CGCGC
BssSI 607 CTCGTG CACGAG(-5/-1)
ClaI 3340 ATCGAT AT^CGAT
DrdI 6781 GACCTCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2926 GACTTCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 6181 AGCGCT AGC^GCT
EcoRI 1021 GAATTC G^AATTC
FbaI 7803 TGATCA T^GATCA
FseI 8180 GGCCGGCC GGCCGG^CC
HindIII 6089 AAGCTT A^AGCTT
MroI 1938 TCCGGA T^CCGGA
PaeR7I 5842 CTCGAG C^TCGAG
PmaCI 1843 CACGTG CAC^GTG
PmlI 1843 CACGTG CAC^GTG
RcaI 6479 TCATGA T^CATGA
SalI 2299 GTCGAC G^TCGAC
SexAI 8084 ACCAGGT A^CC[AT]GGT
SgrAI 3988 CACCGGTG C[GA]^CCGG[CT]G
SspI 1195 AATATT AAT^ATT
Tsp509I 1022 AATT ^AATT
XhoI 5842 CTCGAG C^TCGAG
XmnI 4470 GAACTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 1348 GTAGAC GT^[AC][GT]AC
2299 GTCGAC
AgeI 3530 ACCGGT A^CCGGT
3989 ACCGGT
AspI 1384 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4732 GACTGCGTC
AviII 1847 TGCGCA TGC^GCA
4224 TGCGCA
BbvCI 5799 CCTCAGC CCTCAGC(-5/-2)
7075 GCTGAGG
BstBI 3949 TTCGAA TT^CGAA
6442 TTCGAA
BstEII 2781 GGTCACC G^GT[ACGT]ACC
3652 GGTCACC
BstPI 2781 GGTCACC G^GT[ACGT]ACC
3652 GGTCACC
EcoO65I 2781 GGTCACC G^GT[ACGT]ACC
3652 GGTCACC
EcoRV 3082 GATATC GAT^ATC
5905 GATATC
EcoT22I 3972 ATGCAT ATGCA^T
8254 ATGCAT
FspI 1847 TGCGCA TGC^GCA
4224 TGCGCA
MseI 3512 TTAA T^TAA
4382 TTAA
NsiI 3972 ATGCAT ATGCA^T
8254 ATGCAT
NspV 3949 TTCGAA TT^CGAA
6442 TTCGAA
PflFI 1384 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4732 GACTGCGTC
PinAI 3530 ACCGGT A^CCGGT
3989 ACCGGT
PshAI 2586 GACGGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3088 GACGAGTGTC
SfuI 3949 TTCGAA TT^CGAA
6442 TTCGAA
Tru9I 3512 TTAA T^TAA
4382 TTAA
Tth111I 1384 GACTGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4732 GACTGCGTC
XbaI 2412 TCTAGA T^CTAGA
8196 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 156 enzymes) #
#########################################
Acc65I AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI Asp718I AspHI
AvaI AvaII BalI BamHI BanI
BanII BbeI BbsI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspLU11I BspMI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EagI EarI EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI KasI KpnI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfiI SfoI
SmaI SmlI SphI StuI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII XmaCI
XmaI