Restriction map for
pF1KA1773
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# NO CUT ENZYMES ( 50 enzymes) #
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AclI AcsI AflII AgeI AhdI
ApoI AscI AseI AsnI Asp700I
AspEI BciVI BfrI BsiWI BspDI
BspLU11I Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI Eam1105I
EcoO65I EcoRI EcoT22I FseI NdeI
NotI NruI NsiI PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI ScaI SgrAI SnaBI
SpeI SspI SwaI XhoI XmnI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccI 6664 GTAGAC GT^[AC][GT]AC
AccIII 1870 TCCGGA T^CCGGA
AspI 1252 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5732 TGCGCA TGC^GCA
AvrII 8871 CCTAGG C^CTAGG
BlnI 8871 CCTAGG C^CTAGG
BsaBI 6795 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1870 TCCGGA T^CCGGA
BspEI 1870 TCCGGA T^CCGGA
BsrDI 71 CATTGC GCAATG(2/0)
BstBI 4186 TTCGAA TT^CGAA
EagI 4813 CGGCCG C^GGCCG
EclXI 4813 CGGCCG C^GGCCG
Eco52I 4813 CGGCCG C^GGCCG
EcoRV 8761 GATATC GAT^ATC
FspI 5732 TGCGCA TGC^GCA
MamI 6795 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 8078 CAATTG C^AATTG
MroI 1870 TCCGGA T^CCGGA
MunI 8078 CAATTG C^AATTG
NheI 6211 GCTAGC G^CTAGC
NspV 4186 TTCGAA TT^CGAA
PflFI 1252 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1859 GACTTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 42 GAAGAGC GCTCTTC(1/4)
SfuI 4186 TTCGAA TT^CGAA
Tth111I 1252 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 7677 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 16 enzymes) #
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AatII 3413 GACGTC GACGT^C
4921 GACGTC
Acc65I 453 GGTACC G^GTACC
3675 GGTACC
AflIII 4650 ACGCGT A^C[GA][CT]GT
4734 ACGCGT
Asp718I 453 GGTACC G^GTACC
3675 GGTACC
BglII 3966 AGATCT A^GATCT
6713 AGATCT
BsaWI 884 ACCGGA [AT]^CCGG[AT]
1870 TCCGGA
BsiEI 4548 CGACCG CG[GA][CT]^CG
4813 CGGCCG
BsmBI 5064 CGTCTC CGTCTC(1/5)
7191 CGTCTC
BsmI 6237 GAATGC GAATGC(1/-1)
6314 GCATTC
HpaI 2773 GTTAAC GTT^AAC
5193 GTTAAC
KpnI 453 GGTACC GGTAC^C
3675 GGTACC
KspI 4076 CCGCGG CCGC^GG
4685 CCGCGG
MluI 4650 ACGCGT A^CGCGT
4734 ACGCGT
NcoI 2000 CCATGG C^CATGG
2148 CCATGG
SacII 4076 CCGCGG CCGC^GG
4685 CCGCGG
SphI 4293 GCATGC GCATG^C
8184 GCATGC
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# 3 OR MORE CUT ENZYMES ( 164 enzymes) #
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AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AspHI AvaI AvaII
BalI BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BbvI BcgI
BclI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrBI BsrFI BsrGI BsrI BssHII
BssKI BssSI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I SbfI ScrFI
SexAI SfaNI SfcI SfiI SfoI
SmaI SmlI Sse8387I SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI