Restriction map for
pF1KB7824
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SpeI SphI Sse8387I
SspI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 1648 CGCG CG^CG
AcyI 806 GGCGTC G[GA]^CG[CT]C
AfaI 114 GTAC GT^AC
AlwNI 623 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 1098 GAGCTC G[AT]GC[AT]^C
AspI 1773 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 855 CTCGGG C^[CT]CG[GA]G
BanI 1259 GGCACC G^G[CT][GA]CC
BanII 1098 GAGCTC G[GA]GC[CT]^C
BbsI 1807 GTCTTC GAAGAC(2/6)
BcgI 1227 GCACAGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 356 AGATCT A^GATCT
BpuAI 1807 GTCTTC GAAGAC(2/6)
BsaHI 806 GGCGTC G[GA]^CG[CT]C
BsaI 150 GGTCTC GGTCTC(1/5)
BsgI 876 GTGCAG GTGCAG(16/14)
BsiHKAI 1098 GAGCTC G[AT]GC[AT]^C
BsmFI 660 GGGAC GGGAC(10/14)
BsmI 715 GAATGC GAATGC(1/-1)
BsoBI 855 CTCGGG C^[CT]CG[GA]G
Bsp1407I 113 TGTACA T^GTACA
BsrGI 113 TGTACA T^GTACA
Bst1107I 903 GTATAC GTA^TAC
BstUI 1648 CGCG CG^CG
BstXI 517 CCAGTAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 356 AGATCT [GA]^GATC[CT]
BstZ17I 903 GTATAC GTA^TAC
DraIII 292 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 271 GAATTC G^AATTC
EcoT14I 214 CCTTGG C^C[AT][AT]GG
Hin1I 806 GGCGTC G[GA]^CG[CT]C
MflI 356 AGATCT [GA]^GATC[CT]
MvnI 1648 CGCG CG^CG
NlaIII 703 CATG CATG^
PflFI 1773 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 291 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 106 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 244 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 193 CAGCTG CAG^CTG
RsaI 114 GTAC GT^AC
SacI 1098 GAGCTC GAGCT^C
SmlI 1132 CTTGAG C^T[CT][GA]AG
SnaBI 1543 TACGTA TAC^GTA
SspBI 113 TGTACA T^GTACA
StuI 824 AGGCCT AGG^CCT
StyI 214 CCTTGG C^C[AT][AT]GG
TaqI 188 TCGA T^CGA
TspRI 1303 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1773 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 188 TCGA T^CGA
Van91I 291 CCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 356 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 903 GTATAC GT^[AC][GT]AC
1546 GTAGAC
AcsI 271 GAATTC [GA]^AATT[CT]
479 GAATTT
ApoI 271 GAATTC [GA]^AATT[CT]
479 GAATTT
Asp700I 232 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1032 GAAGCCCTTC
BalI 342 TGGCCA TGG^CCA
1572 TGGCCA
BbvI 315 GCAGC GCAGC(8/12)
1155 GCAGC
BsaAI 641 TACGTG [CT]AC^GT[GA]
1543 TACGTA
Cac8I 311 GCTCGC GC[ACGT]^[ACGT]GC
1479 GCGTGC
CfoI 334 GCGC GCG^C
1647 GCGC
DpnII 357 GATC ^GATC
740 GATC
EaeI 342 TGGCCA [CT]^GGCC[GA]
1572 TGGCCA
FauI 719 GCGGG CCCGC(4/6)
1311 GCGGG
HgaI 1017 GACGC GACGC(5/10)
807 GCGTC
HhaI 334 GCGC GCG^C
1647 GCGC
HinP1I 334 GCGC G^CGC
1647 GCGC
MaeII 642 ACGT A^CGT
1544 ACGT
MboI 357 GATC ^GATC
740 GATC
MluNI 342 TGGCCA TGG^CCA
1572 TGGCCA
MscI 342 TGGCCA TGG^CCA
1572 TGGCCA
MslI 505 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
785 CATACCTGTG
MspA1I 193 CAGCTG C[AC]G^C[GT]G
1684 CAGCGG
NdeII 357 GATC ^GATC
740 GATC
PleI 11 GACTC GAGTC(4/5)
579 GAGTC
PstI 374 CTGCAG CTGCA^G
1716 CTGCAG
Sau3AI 357 GATC ^GATC
740 GATC
SexAI 293 ACCTGGT A^CC[AT]GGT
1178 ACCAGGT
TfiI 625 GAATC G^A[AT]TC
933 GATTC
TseI 315 GCAGC G^C[AT]GC
1155 GCAGC
XmnI 232 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1032 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AluI AvaII BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstEII BstF5I BstNI BstPI Cfr13I
DdeI DraII EcoO109I EcoO65I EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboII MnlI MseI MspI MvaI
MwoI NciI NlaIV Sau96I ScrFI
SfaNI SfcI Tru9I Tsp45I Tsp509I