Restriction map for
pF1KA1689
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# NO CUT ENZYMES ( 72 enzymes) #
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AatII AccIII AflII AgeI AhdI
Aor51HI AscI AspEI AspI AviII
BalI BamHI BcnI BfrI BglI
BseAI BsiEI BsiWI BspDI BspEI
BsrFI BssHII BstAPI BstEII BstPI
Cfr10I ClaI CpoI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FseI FspI MfeI
MluI MluNI MroI MscI MunI
NaeI NciI NdeI NgoMIV NotI
NruI PacI PflFI PflMI PinAI
PmeI PvuI PvuII RsrII SalI
SbfI SfiI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I Van91I
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AclI 7154 AACGTT AA^CGTT
AflIII 6599 ACATGT A^C[GA][CT]GT
Alw44I 7201 GTGCAC G^TGCAC
ApaLI 7201 GTGCAC G^TGCAC
Asp700I 4954 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 737 CTCGAG C^[CT]CG[GA]G
AvrII 202 CCTAGG C^CTAGG
BbeI 52 GGCGCC GGCGC^C
BbrPI 1960 CACGTG CAC^GTG
BcgI 1092 CGATTTTTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3130 GTATCC GTATCC(6/5)
BlnI 202 CCTAGG C^CTAGG
BsaAI 1960 CACGTG [CT]AC^GT[GA]
BsaWI 3361 ACCGGA [AT]^CCGG[AT]
BsmBI 3181 GAGACG CGTCTC(1/5)
BsoBI 737 CTCGAG C^[CT]CG[GA]G
BspLU11I 6599 ACATGT A^CATGT
BssSI 3557 CACGAG CACGAG(-5/-1)
Bst1107I 6744 GTATAC GTA^TAC
BstBI 1708 TTCGAA TT^CGAA
BstZ17I 6744 GTATAC GTA^TAC
EcoRI 1926 GAATTC G^AATTC
HgaI 2742 GACGC GACGC(5/10)
HindIII 4475 AAGCTT A^AGCTT
HpaI 4966 GTTAAC GTT^AAC
KasI 52 GGCGCC G^GCGCC
KspI 140 CCGCGG CCGC^GG
MspA1I 140 CCGCGG C[AC]G^C[GT]G
NarI 52 GGCGCC GG^CGCC
NheI 3720 GCTAGC G^CTAGC
NspV 1708 TTCGAA TT^CGAA
PaeR7I 737 CTCGAG C^TCGAG
PmaCI 1960 CACGTG CAC^GTG
PmlI 1960 CACGTG CAC^GTG
PpuMI 1464 GGGTCCT [GA]G^G[AT]CC[CT]
Psp1406I 7154 AACGTT AA^CGTT
SacI 6582 GAGCTC GAGCT^C
SacII 140 CCGCGG CCGC^GG
SexAI 5925 ACCAGGT A^CC[AT]GGT
SfoI 52 GGCGCC GGC^GCC
SfuI 1708 TTCGAA TT^CGAA
SphI 7360 GCATGC GCATG^C
XcmI 5582 CCAAGGCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 737 CTCGAG C^TCGAG
XmnI 4954 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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Acc65I 2580 GGTACC G^GTACC
5929 GGTACC
AccII 141 CGCG CG^CG
853 CGCG
AcyI 52 GGCGCC G[GA]^CG[CT]C
2742 GACGCC
AocI 7274 CCTCAGG CC^T[ACGT]AGG
7390 CCTCAGG
ApaI 6637 GGGCCC GGGCC^C
7796 GGGCCC
Asp718I 2580 GGTACC G^GTACC
5929 GGTACC
BbvCI 1747 GCTGAGG CCTCAGC(-5/-2)
3577 GCTGAGG
BclI 1806 TGATCA T^GATCA
7752 TGATCA
BsaHI 52 GGCGCC G[GA]^CG[CT]C
2742 GACGCC
BspHI 4170 TCATGA T^CATGA
6108 TCATGA
BspMI 1353 GCAGGT ACCTGC(4/8)
2143 GCAGGT
BsrBI 179 GAGCGG CCGCTC(-3/-3)
4014 GAGCGG
BsrDI 2710 GCAATG GCAATG(2/0)
4022 GCAATG
BstUI 141 CGCG CG^CG
853 CGCG
Bsu36I 7274 CCTCAGG CC^T[ACGT]AGG
7390 CCTCAGG
Eco81I 7274 CCTCAGG CC^T[ACGT]AGG
7390 CCTCAGG
EcoRV 4488 GATATC GAT^ATC
7843 GATATC
FbaI 1806 TGATCA T^GATCA
7752 TGATCA
HaeII 52 GGCGCC [GA]GCGC^[CT]
1076 AGCGCC
HapII 109 CCGG C^CGG
3362 CCGG
Hin1I 52 GGCGCC G[GA]^CG[CT]C
2742 GACGCC
HpaII 109 CCGG C^CGG
3362 CCGG
KpnI 2580 GGTACC GGTAC^C
5929 GGTACC
MspI 109 CCGG C^CGG
3362 CCGG
MvnI 141 CGCG CG^CG
853 CGCG
NcoI 904 CCATGG C^CATGG
7031 CCATGG
PshAI 89 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
639 GACTCCAGTC
PstI 2068 CTGCAG CTGCA^G
5155 CTGCAG
RcaI 4170 TCATGA T^CATGA
6108 TCATGA
SspI 7720 AATATT AAT^ATT
7829 AATATT
StuI 40 AGGCCT AGG^CCT
1510 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AseI AsnI
AspHI AvaII BanI BanII BbsI
BbvI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PleI PshBI RsaI
SapI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII