##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AccIII AclI AflII AscI AseI
AsnI AvrII BclI BfrI BlnI
BsaI BseAI BspEI BspHI BspLU11I
Bst1107I BstXI BstZ17I CpoI DraI
DrdI EcoRV EcoT22I FbaI HincII
HindII HpaI MfeI MluI MroI
MunI NdeI NheI NruI NsiI
PacI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SfiI SnaBI SpeI Sse8387I
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AflIII 1655 ACGTGT A^C[GA][CT]GT
AgeI 1812 ACCGGT A^CCGGT
BbrPI 2332 CACGTG CAC^GTG
BciVI 3519 GGATAC GTATCC(6/5)
BsaBI 4728 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 2766 CGTACG C^GTACG
BspDI 5322 ATCGAT AT^CGAT
BssHII 5580 GCGCGC G^CGCGC
BstBI 3289 TTCGAA TT^CGAA
ClaI 5322 ATCGAT AT^CGAT
FseI 1576 GGCCGGCC GGCCGG^CC
HindIII 5434 AAGCTT A^AGCTT
MamI 4728 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1379 TTAA T^TAA
NotI 4867 GCGGCCGC GC^GGCCGC
NspV 3289 TTCGAA TT^CGAA
PaeR7I 4900 CTCGAG C^TCGAG
PinAI 1812 ACCGGT A^CCGGT
PmaCI 2332 CACGTG CAC^GTG
PmlI 2332 CACGTG CAC^GTG
SfuI 3289 TTCGAA TT^CGAA
SgrAI 1811 CACCGGTG C[GA]^CCGG[CT]G
Tru9I 1379 TTAA T^TAA
XhoI 4900 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 3845 GACGTC GACGT^C
5389 GACGTC
Acc65I 3622 GGTACC G^GTACC
3979 GGTACC
AccI 1819 GTCTAC GT^[AC][GT]AC
5116 GTCTAC
Aor51HI 1219 AGCGCT AGC^GCT
3809 AGCGCT
Asp718I 3622 GGTACC G^GTACC
3979 GGTACC
AviII 1700 TGCGCA TGC^GCA
3119 TGCGCA
BamHI 293 GGATCC G^GATCC
1973 GGATCC
BbvCI 3547 GCTGAGG CCTCAGC(-5/-2)
4606 GCTGAGG
BglII 872 AGATCT A^GATCT
5342 AGATCT
BsmBI 479 GAGACG CGTCTC(1/5)
5686 GAGACG
BsmI 271 GAATGC GAATGC(1/-1)
3382 GCATTC
BsrBI 966 CCGCTC CCGCTC(-3/-3)
622 GAGCGG
DraIII 2329 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
3157 CACTACGTG
Eco47III 1219 AGCGCT AGC^GCT
3809 AGCGCT
EcoRI 229 GAATTC G^AATTC
3127 GAATTC
FspI 1700 TGCGCA TGC^GCA
3119 TGCGCA
KpnI 3622 GGTACC GGTAC^C
3979 GGTACC
NcoI 3644 CCATGG C^CATGG
5084 CCATGG
SexAI 221 ACCTGGT A^CC[AT]GGT
4792 ACCTGGT
SphI 1172 GCATGC GCATG^C
2087 GCATGC
TfiI 1593 GATTC G^A[AT]TC
4038 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 160 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AhdI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI Asp700I
AspEI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EagI Eam1105I EarI EclXI Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI PvuII RsaI SacI SacII
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI SspBI
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmaCI XmaI XmnI