Restriction map for
pF1KA1375
##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BglI BlnI BsaAI BsaHI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
DrdI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI Hin1I KasI KpnI
KspI MluI MroI NaeI NarI
NgoMIV NotI NruI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SwaI Tth111I
Van91I XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AclI 1421 AACGTT AA^CGTT
AhdI 555 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 988 GATCC GGATC(4/5)
AspEI 555 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 472 GGTGCC G^G[CT][GA]CC
BbsI 2094 GAAGAC GAAGAC(2/6)
BbvCI 2569 CCTCAGC CCTCAGC(-5/-2)
BpuAI 2094 GAAGAC GAAGAC(2/6)
BsaBI 982 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1890 GGTCTC GGTCTC(1/5)
BsmI 1590 GAATGC GAATGC(1/-1)
BspHI 957 TCATGA T^CATGA
BspMI 2172 ACCTGC ACCTGC(4/8)
BsrDI 2136 CATTGC GCAATG(2/0)
BssHII 188 GCGCGC G^CGCGC
BssSI 181 CACGAG CACGAG(-5/-1)
DraI 600 TTTAAA TTT^AAA
Eam1105I 555 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 100 GAAGAG CTCTTC(1/4)
EcoRV 2423 GATATC GAT^ATC
FauI 95 GCGGG CCCGC(4/6)
HaeII 2226 AGCGCC [GA]GCGC^[CT]
HgaI 198 GACGC GACGC(5/10)
HindIII 342 AAGCTT A^AGCTT
HpaI 1132 GTTAAC GTT^AAC
Ksp632I 100 GAAGAG CTCTTC(1/4)
MaeII 1422 ACGT A^CGT
MamI 982 GATCTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 2453 CCATGG C^CATGG
NdeI 1976 CATATG CA^TATG
PaeR7I 193 CTCGAG C^TCGAG
Psp1406I 1421 AACGTT AA^CGTT
PvuII 22 CAGCTG CAG^CTG
RcaI 957 TCATGA T^CATGA
SapI 100 GAAGAGC GCTCTTC(1/4)
ScaI 113 AGTACT AGT^ACT
SmaI 2538 CCCGGG CCC^GGG
SphI 834 GCATGC GCATG^C
SspI 1882 AATATT AAT^ATT
StuI 2269 AGGCCT AGG^CCT
TfiI 1849 GAATC G^A[AT]TC
Tsp45I 1550 GTGAC ^GT[GC]AC
XbaI 984 TCTAGA T^CTAGA
XcmI 459 CCAGTTTGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 193 CTCGAG C^TCGAG
XmaCI 2538 CCCGGG C^CCGGG
XmaI 2538 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflII 510 CTTAAG C^TTAAG
1941 CTTAAG
AvaI 193 CTCGAG C^[CT]CG[GA]G
2538 CCCGGG
BalI 50 TGGCCA TGG^CCA
2478 TGGCCA
BanII 133 GAGCTC G[GA]GC[CT]^C
2300 GAGCTC
BclI 216 TGATCA T^GATCA
1299 TGATCA
BfrI 510 CTTAAG C^TTAAG
1941 CTTAAG
BlpI 135 GCTCAGC GC^T[ACGT]AGC
2372 GCTCAGC
Bpu1102I 135 GCTCAGC GC^T[ACGT]AGC
2372 GCTCAGC
BsiEI 162 CGGTCG CG[GA][CT]^CG
524 CGACCG
BsmBI 196 GAGACG CGTCTC(1/5)
493 GAGACG
BsoBI 193 CTCGAG C^[CT]CG[GA]G
2538 CCCGGG
CelII 135 GCTCAGC GC^T[ACGT]AGC
2372 GCTCAGC
DraII 553 GGGACCC [GA]G^G[ACGT]CC[CT]
2541 GGGACCC
DsaI 527 CCGTGG C^C[GA][CT]GG
2453 CCATGG
EaeI 50 TGGCCA [CT]^GGCC[GA]
2478 TGGCCA
EcoO109I 553 GGGACCC [GA]G^G[ACGT]CC[CT]
2541 GGGACCC
EcoT14I 713 CCAAGG C^C[AT][AT]GG
2453 CCATGG
FbaI 216 TGATCA T^GATCA
1299 TGATCA
HapII 1024 CCGG C^CGG
2539 CCGG
HincII 1132 GTTAAC GT[CT]^[GA]AC
2037 GTTGAC
HindII 1132 GTTAAC GT[CT]^[GA]AC
2037 GTTGAC
HpaII 1024 CCGG C^CGG
2539 CCGG
MluNI 50 TGGCCA TGG^CCA
2478 TGGCCA
MscI 50 TGGCCA TGG^CCA
2478 TGGCCA
MspI 1024 CCGG C^CGG
2539 CCGG
NheI 391 GCTAGC G^CTAGC
1185 GCTAGC
NspI 834 GCATGC [GA]CATG^[CT]
2202 ACATGC
PpuMI 553 GGGACCC [GA]G^G[AT]CC[CT]
2541 GGGACCC
PstI 659 CTGCAG CTGCA^G
926 CTGCAG
SacI 133 GAGCTC GAGCT^C
2300 GAGCTC
SfcI 659 CTGCAG C^T[GA][CT]AG
926 CTGCAG
StyI 713 CCAAGG C^C[AT][AT]GG
2453 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwNI ApoI AspHI AvaII BbvI
BcnI BfaI BglII BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
MaeI MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MunI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SmlI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII