Restriction map for
pF1KA1315
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI Alw44I AocI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BglI BlnI BlpI Bpu1102I BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspMI BsrBI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV FauI FseI FspI HgaI
Hin1I KasI KpnI KspI MluI
MluNI MroI MscI MvnI NaeI
NarI NgoMIV NheI NotI NruI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SgrAI SmaI SpeI Sse8387I
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 26 enzymes) #
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AclI 1887 AACGTT AA^CGTT
BciVI 2187 GGATAC GTATCC(6/5)
BcnI 5799 CCGGG CC^[GC]GG
BsaAI 5047 TACGTA [CT]AC^GT[GA]
BseRI 59 CTCCTC GAGGAG(10/8)
BsgI 663 CTGCAC GTGCAG(16/14)
BsmFI 5476 GGGAC GGGAC(10/14)
BsmI 3516 GAATGC GAATGC(1/-1)
BsrFI 4188 GCCGGT [GA]^CCGG[CT]
BstXI 5081 CCAAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 4188 GCCGGT [GA]^CCGG[CT]
DraIII 2909 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1868 CCATGG C^C[GA][CT]GG
HpaI 3982 GTTAAC GTT^AAC
MfeI 2300 CAATTG C^AATTG
MunI 2300 CAATTG C^AATTG
NciI 5799 CCGGG CC^[GC]GG
NcoI 1868 CCATGG C^CATGG
NdeI 1811 CATATG CA^TATG
PacI 3235 TTAATTAA TTAAT^TAA
PpuMI 1655 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1887 AACGTT AA^CGTT
SacI 4845 GAGCTC GAGCT^C
SnaBI 5047 TACGTA TAC^GTA
StuI 5347 AGGCCT AGG^CCT
SwaI 5150 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 1003 GTAGAC GT^[AC][GT]AC
4233 GTCTAC
Aor51HI 127 AGCGCT AGC^GCT
3752 AGCGCT
AseI 2107 ATTAAT AT^TAAT
3183 ATTAAT
AsnI 2107 ATTAAT AT^TAAT
3183 ATTAAT
Asp700I 5029 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5507 GAAACATTTC
BanII 2014 GAGCCC G[GA]GC[CT]^C
4845 GAGCTC
BspHI 2505 TCATGA T^CATGA
4833 TCATGA
BsrDI 4184 CATTGC GCAATG(2/0)
1615 GCAATG
BstAPI 4283 GCAAGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5710 GCAGCACTTGC
BstBI 437 TTCGAA TT^CGAA
906 TTCGAA
CfoI 128 GCGC GCG^C
3753 GCGC
DraII 1655 AGGACCT [GA]G^G[ACGT]CC[CT]
5645 AGGCCCC
Eco47III 127 AGCGCT AGC^GCT
3752 AGCGCT
EcoO109I 1655 AGGACCT [GA]G^G[ACGT]CC[CT]
5645 AGGCCCC
EcoRI 2778 GAATTC G^AATTC
5095 GAATTC
HaeII 127 AGCGCT [GA]GCGC^[CT]
3752 AGCGCT
HapII 4189 CCGG C^CGG
5799 CCGG
HhaI 128 GCGC GCG^C
3753 GCGC
HinP1I 128 GCGC G^CGC
3753 GCGC
HindIII 3433 AAGCTT A^AGCTT
3465 AAGCTT
HpaII 4189 CCGG C^CGG
5799 CCGG
MspI 4189 CCGG C^CGG
5799 CCGG
NspV 437 TTCGAA TT^CGAA
906 TTCGAA
PflMI 2469 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2908 CCACGACGTGG
PshBI 2107 ATTAAT AT^TAAT
3183 ATTAAT
RcaI 2505 TCATGA T^CATGA
4833 TCATGA
SfuI 437 TTCGAA TT^CGAA
906 TTCGAA
SphI 3837 GCATGC GCATG^C
4587 GCATGC
Van91I 2469 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2908 CCACGACGTGG
XmnI 5029 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5507 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BbsI BbvI BclI BfaI
BfrI BglII BmyI BpmI BpuAI
BsaBI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BspLU11I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRII EcoT14I EcoT22I
FbaI Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NdeII NlaIII
NlaIV NsiI NspI PleI PvuII
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII