Restriction map for
pF1KA1113
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# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspHI AspI AvrII BamHI BbeI
BbrPI BcgI BfrI BglI BlnI
BsaAI BsaBI BsaWI BseAI BsiHKAI
BsiWI BsmBI BspDI BspEI BspHI
BsrDI BssSI Bst1107I BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRV EcoT14I FseI HpaI KasI
KpnI MamI MluI MroI NarI
NcoI NdeI NheI NotI NruI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SbfI SexAI SfiI SfoI SgrAI
SnaBI Sse8387I StyI SwaI Tth111I
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcyI 285 GACGCC G[GA]^CG[CT]C
AviII 2862 TGCGCA TGC^GCA
BalI 1091 TGGCCA TGG^CCA
BanI 539 GGTGCC G^G[CT][GA]CC
BbvCI 163 GCTGAGG CCTCAGC(-5/-2)
BciVI 1802 GGATAC GTATCC(6/5)
BsaHI 285 GACGCC G[GA]^CG[CT]C
BsiEI 176 CGGCCG CG[GA][CT]^CG
Bsp1407I 831 TGTACA T^GTACA
BsrGI 831 TGTACA T^GTACA
BssHII 56 GCGCGC G^CGCGC
BstBI 2591 TTCGAA TT^CGAA
DraIII 1334 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 176 CGGCCG C^GGCCG
EarI 3283 GAAGAG CTCTTC(1/4)
EclXI 176 CGGCCG C^GGCCG
Eco52I 176 CGGCCG C^GGCCG
EcoNI 2155 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2941 GAATTC G^AATTC
EcoT22I 2486 ATGCAT ATGCA^T
FspI 2862 TGCGCA TGC^GCA
Hin1I 285 GACGCC G[GA]^CG[CT]C
HincII 809 GTCAAC GT[CT]^[GA]AC
HindII 809 GTCAAC GT[CT]^[GA]AC
Ksp632I 3283 GAAGAG CTCTTC(1/4)
MluNI 1091 TGGCCA TGG^CCA
MscI 1091 TGGCCA TGG^CCA
NsiI 2486 ATGCAT ATGCA^T
NspV 2591 TTCGAA TT^CGAA
PstI 2525 CTGCAG CTGCA^G
PvuII 2256 CAGCTG CAG^CTG
SfuI 2591 TTCGAA TT^CGAA
SmaI 495 CCCGGG CCC^GGG
SpeI 652 ACTAGT A^CTAGT
SphI 1875 GCATGC GCATG^C
SspBI 831 TGTACA T^GTACA
SspI 1826 AATATT AAT^ATT
StuI 1209 AGGCCT AGG^CCT
Tsp45I 2120 GTCAC ^GT[GC]AC
XbaI 2469 TCTAGA T^CTAGA
XmaCI 495 CCCGGG C^CCGGG
XmaI 495 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 745 AAATTT [GA]^AATT[CT]
2941 GAATTC
ApaI 82 GGGCCC GGGCC^C
181 GGGCCC
ApoI 745 AAATTT [GA]^AATT[CT]
2941 GAATTC
AseI 1117 ATTAAT AT^TAAT
3160 ATTAAT
AsnI 1117 ATTAAT AT^TAAT
3160 ATTAAT
BglII 2691 AGATCT A^GATCT
3236 AGATCT
BlpI 480 GCTCAGC GC^T[ACGT]AGC
2559 GCTCAGC
Bpu1102I 480 GCTCAGC GC^T[ACGT]AGC
2559 GCTCAGC
BsaI 2220 GAGACC GGTCTC(1/5)
3363 GAGACC
BsgI 674 GTGCAG GTGCAG(16/14)
1030 GTGCAG
BsmI 559 GAATGC GAATGC(1/-1)
1782 GAATGC
BsrBI 314 CCGCTC CCGCTC(-3/-3)
33 GAGCGG
BstAPI 387 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1716 GCAAACAATGC
BstXI 519 CCAGCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2054 CCATACAGTTGG
BstYI 2691 AGATCT [GA]^GATC[CT]
3236 AGATCT
CelII 480 GCTCAGC GC^T[ACGT]AGC
2559 GCTCAGC
HaeII 160 GGCGCT [GA]GCGC^[CT]
473 AGCGCC
HgaI 285 GACGC GACGC(5/10)
1317 GCGTC
HindIII 941 AAGCTT A^AGCTT
3025 AAGCTT
KspI 116 CCGCGG CCGC^GG
203 CCGCGG
MaeII 1371 ACGT A^CGT
2901 ACGT
MfeI 965 CAATTG C^AATTG
1256 CAATTG
MflI 2691 AGATCT [GA]^GATC[CT]
3236 AGATCT
MslI 114 CACCGCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2211 CACACCTGTG
MunI 965 CAATTG C^AATTG
1256 CAATTG
PflMI 1388 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1444 CCAGCTCCTGG
PshBI 1117 ATTAAT AT^TAAT
3160 ATTAAT
SacII 116 CCGCGG CCGC^GG
203 CCGCGG
ScaI 2230 AGTACT AGT^ACT
2239 AGTACT
SmlI 991 CTTGAG C^T[CT][GA]AG
2076 CTCAAG
Van91I 1388 CCACCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1444 CCAGCTCCTGG
XcmI 2653 CCAAATGAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2764 CCAAGTGGGGACTGG
XhoII 2691 AGATCT [GA]^GATC[CT]
3236 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI Asp700I AvaI AvaII
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII FauI FbaI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XmnI