Restriction map for
pF1KB7820
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII AclI AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AviII
AvrII BbrPI BbsI BcgI BciVI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsiEI BsiWI
BsmBI BsmI BspDI BspLU11I BssHII
BssSI Bst1107I BstEII BstPI BstZ17I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV EcoT22I FseI FspI
MaeII MamI MfeI MluI MunI
NdeI NheI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RsrII SapI SbfI
ScaI SfiI SnaBI SphI Sse8387I
SspI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 211 GTCGAC GT^[AC][GT]AC
AccII 394 CGCG CG^CG
AccIII 383 TCCGGA T^CCGGA
AflII 1639 CTTAAG C^TTAAG
AocI 1668 CCTGAGG CC^T[ACGT]AGG
Asp700I 447 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 864 TGGCCA TGG^CCA
BbeI 43 GGCGCC GGCGC^C
BbvCI 661 GCTGAGG CCTCAGC(-5/-2)
BclI 125 TGATCA T^GATCA
BfrI 1639 CTTAAG C^TTAAG
BsaWI 383 TCCGGA [AT]^CCGG[AT]
BseAI 383 TCCGGA T^CCGGA
BseRI 1724 CTCCTC GAGGAG(10/8)
BsgI 821 CTGCAC GTGCAG(16/14)
Bsp1407I 326 TGTACA T^GTACA
BspEI 383 TCCGGA T^CCGGA
BspHI 1728 TCATGA T^CATGA
BsrBI 1105 GAGCGG CCGCTC(-3/-3)
BsrFI 46 GCCGGC [GA]^CCGG[CT]
BsrGI 326 TGTACA T^GTACA
BstBI 454 TTCGAA TT^CGAA
BstUI 394 CGCG CG^CG
BstXI 926 CCAATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1668 CCTGAGG CC^T[ACGT]AGG
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
DraII 541 AGGCCCC [GA]G^G[ACGT]CC[CT]
Eco81I 1668 CCTGAGG CC^T[ACGT]AGG
EcoNI 1554 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 541 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1691 GAATTC G^AATTC
FbaI 125 TGATCA T^GATCA
HgaI 50 GCGTC GACGC(5/10)
HindIII 688 AAGCTT A^AGCTT
HpaI 520 GTTAAC GTT^AAC
KasI 43 GGCGCC G^GCGCC
KspI 393 CCGCGG CCGC^GG
MluNI 864 TGGCCA TGG^CCA
MroI 383 TCCGGA T^CCGGA
MscI 864 TGGCCA TGG^CCA
MvnI 394 CGCG CG^CG
NaeI 46 GCCGGC GCC^GGC
NarI 43 GGCGCC GG^CGCC
NcoI 1001 CCATGG C^CATGG
NgoMIV 46 GCCGGC G^CCGGC
NspI 215 ACATGC [GA]CATG^[CT]
NspV 454 TTCGAA TT^CGAA
PflMI 871 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1728 TCATGA T^CATGA
SacI 1468 GAGCTC GAGCT^C
SacII 393 CCGCGG CCGC^GG
SalI 211 GTCGAC G^TCGAC
SexAI 302 ACCTGGT A^CC[AT]GGT
SfoI 43 GGCGCC GGC^GCC
SfuI 454 TTCGAA TT^CGAA
SgrAI 45 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 1654 ACTAGT A^CTAGT
SspBI 326 TGTACA T^GTACA
StuI 606 AGGCCT AGG^CCT
Van91I 871 CCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1140 TCTAGA T^CTAGA
XcmI 867 CCAGCCAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 447 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Acc65I 1087 GGTACC G^GTACC
1245 GGTACC
AcyI 43 GGCGCC G[GA]^CG[CT]C
49 GGCGTC
Asp718I 1087 GGTACC G^GTACC
1245 GGTACC
AspHI 884 GTGCTC G[AT]GC[AT]^C
1468 GAGCTC
AvaI 54 CCCGGG C^[CT]CG[GA]G
415 CCCGGG
BamHI 386 GGATCC G^GATCC
1109 GGATCC
BanII 100 GAGCCC G[GA]GC[CT]^C
1468 GAGCTC
BglI 866 GCCAGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1039 GCCAACATGGC
BsaHI 43 GGCGCC G[GA]^CG[CT]C
49 GGCGTC
BsiHKAI 884 GTGCTC G[AT]GC[AT]^C
1468 GAGCTC
BsoBI 54 CCCGGG C^[CT]CG[GA]G
415 CCCGGG
BspMI 917 ACCTGC ACCTGC(4/8)
378 GCAGGT
BsrDI 1310 CATTGC GCAATG(2/0)
1006 GCAATG
BstAPI 991 GCATCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1305 GCAGGCATTGC
DraIII 301 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
319 CACACTGTG
EaeI 252 CGGCCA [CT]^GGCC[GA]
864 TGGCCA
Hin1I 43 GGCGCC G[GA]^CG[CT]C
49 GGCGTC
KpnI 1087 GGTACC GGTAC^C
1245 GGTACC
MslI 1002 CATGGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1729 CATGATAGTG
PvuII 647 CAGCTG CAG^CTG
1291 CAGCTG
SmaI 54 CCCGGG CCC^GGG
415 CCCGGG
SmlI 295 CTCAAG C^T[CT][GA]AG
1639 CTTAAG
Tsp45I 1272 GTCAC ^GT[GC]AC
1658 GTGAC
XmaCI 54 CCCGGG C^CCGGG
415 CCCGGG
XmaI 54 CCCGGG C^CCGGG
415 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BbvI
BcnI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp509I TspRI TthHB8I
XhoII