Restriction map for
pF1KA0540
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# NO CUT ENZYMES ( 49 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AseI AsnI Asp700I AspI AvrII
BamHI BfrI BlnI BsaBI BsaWI
BseAI BsiWI BspEI CpoI DraI
EcoT22I FseI HpaI MamI MluI
MroI NdeI NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I RsrII SalI SfiI
SgrAI SnaBI SpeI SspI SwaI
TfiI Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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Acc65I 1773 GGTACC G^GTACC
AscI 5331 GGCGCGCC GG^CGCGCC
Asp718I 1773 GGTACC G^GTACC
BclI 7730 TGATCA T^GATCA
BglII 3720 AGATCT A^GATCT
BspDI 5977 ATCGAT AT^CGAT
BspHI 1172 TCATGA T^CATGA
BspLU11I 3131 ACATGT A^CATGT
Bst1107I 5836 GTATAC GTA^TAC
BstBI 5173 TTCGAA TT^CGAA
BstZ17I 5836 GTATAC GTA^TAC
ClaI 5977 ATCGAT AT^CGAT
DrdI 3325 GACGTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 6216 GATATC GAT^ATC
FbaI 7730 TGATCA T^GATCA
HincII 330 GTTGAC GT[CT]^[GA]AC
HindII 330 GTTGAC GT[CT]^[GA]AC
HindIII 6426 AAGCTT A^AGCTT
KpnI 1773 GGTACC GGTAC^C
MfeI 990 CAATTG C^AATTG
MunI 990 CAATTG C^AATTG
NspV 5173 TTCGAA TT^CGAA
PvuI 3528 CGATCG CGAT^CG
RcaI 1172 TCATGA T^CATGA
ScaI 6230 AGTACT AGT^ACT
SfuI 5173 TTCGAA TT^CGAA
XbaI 750 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcsI 535 GAATTC [GA]^AATT[CT]
6652 GAATTC
Alw44I 1968 GTGCAC G^TGCAC
2463 GTGCAC
ApaLI 1968 GTGCAC G^TGCAC
2463 GTGCAC
ApoI 535 GAATTC [GA]^AATT[CT]
6652 GAATTC
BciVI 1842 GGATAC GTATCC(6/5)
2182 GGATAC
Bsp1407I 1782 TGTACA T^GTACA
1892 TGTACA
BsrGI 1782 TGTACA T^GTACA
1892 TGTACA
EcoRI 535 GAATTC G^AATTC
6652 GAATTC
KspI 4183 CCGCGG CCGC^GG
7749 CCGCGG
MseI 851 TTAA T^TAA
1205 TTAA
NaeI 2063 GCCGGC GCC^GGC
3895 GCCGGC
NgoMIV 2063 GCCGGC G^CCGGC
3895 GCCGGC
NheI 1515 GCTAGC G^CTAGC
7161 GCTAGC
PshAI 3775 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6024 GACCCCAGTC
SacII 4183 CCGCGG CCGC^GG
7749 CCGCGG
SexAI 2081 ACCTGGT A^CC[AT]GGT
6098 ACCAGGT
SmaI 1046 CCCGGG CCC^GGG
7562 CCCGGG
SspBI 1782 TGTACA T^GTACA
1892 TGTACA
Tru9I 851 TTAA T^TAA
1205 TTAA
XmaCI 1046 CCCGGG C^CCGGG
7562 CCCGGG
XmaI 1046 CCCGGG C^CCGGG
7562 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 161 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AhdI AluI AlwI AlwNI
AocI Aor51HI ApaI AspEI AspHI
AvaI AvaII AviII BalI BanI
BanII BbeI BbrPI BbsI BbvCI
BbvI BcgI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssHII BssKI BssSI BstAPI
BstEII BstF5I BstNI BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I SbfI ScrFI SfaNI
SfcI SfoI SmlI SphI Sse8387I
StuI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII