Restriction map for
pF1KA0246
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# NO CUT ENZYMES ( 48 enzymes) #
##################################
AclI AcsI AhdI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
BsaBI BsiWI BspDI BspHI Bst1107I
BstZ17I ClaI DraI Eam1105I Eco47III
EcoRI EcoRV HincII HindII HpaI
MamI MluI NotI NruI PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RcaI SalI SbfI ScaI
SgrAI SpeI Sse8387I SspI SwaI
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 5748 GACGTC GACGT^C
Acc65I 1724 GGTACC G^GTACC
AflII 3022 CTTAAG C^TTAAG
Asp718I 1724 GGTACC G^GTACC
AspI 5113 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4908 TGCGCA TGC^GCA
AvrII 4261 CCTAGG C^CTAGG
BbeI 7407 GGCGCC GGCGC^C
BclI 576 TGATCA T^GATCA
BfrI 3022 CTTAAG C^TTAAG
BlnI 4261 CCTAGG C^CTAGG
BsmBI 1209 CGTCTC CGTCTC(1/5)
Bsp1407I 6265 TGTACA T^GTACA
BsrGI 6265 TGTACA T^GTACA
BssHII 5059 GCGCGC G^CGCGC
BstBI 5447 TTCGAA TT^CGAA
BstEII 999 GGTGACC G^GT[ACGT]ACC
BstPI 999 GGTGACC G^GT[ACGT]ACC
CpoI 2603 CGGACCG CG^G[AT]CCG
EcoO65I 999 GGTGACC G^GT[ACGT]ACC
FbaI 576 TGATCA T^GATCA
FseI 5238 GGCCGGCC GGCCGG^CC
FspI 4908 TGCGCA TGC^GCA
HindIII 5906 AAGCTT A^AGCTT
KasI 7407 GGCGCC G^GCGCC
KpnI 1724 GGTACC GGTAC^C
NarI 7407 GGCGCC GG^CGCC
NdeI 4658 CATATG CA^TATG
NheI 5370 GCTAGC G^CTAGC
NspV 5447 TTCGAA TT^CGAA
PflFI 5113 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 2603 CGGACCG CG^G[AT]CCG
SapI 3876 GAAGAGC GCTCTTC(1/4)
SfiI 7281 GGCCCCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 7407 GGCGCC GGC^GCC
SfuI 5447 TTCGAA TT^CGAA
SnaBI 6496 TACGTA TAC^GTA
SspBI 6265 TGTACA T^GTACA
Tth111I 5113 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccIII 4604 TCCGGA T^CCGGA
5325 TCCGGA
AgeI 461 ACCGGT A^CCGGT
980 ACCGGT
BciVI 556 GGATAC GTATCC(6/5)
4333 GGATAC
BglII 4863 AGATCT A^GATCT
5213 AGATCT
BlpI 431 GCTCAGC GC^T[ACGT]AGC
3099 GCTGAGC
Bpu1102I 431 GCTCAGC GC^T[ACGT]AGC
3099 GCTGAGC
BseAI 4604 TCCGGA T^CCGGA
5325 TCCGGA
BspEI 4604 TCCGGA T^CCGGA
5325 TCCGGA
BspLU11I 3928 ACATGT A^CATGT
4696 ACATGT
CelII 431 GCTCAGC GC^T[ACGT]AGC
3099 GCTGAGC
DrdI 3052 GACCGCCGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6509 GACTGCAGTGTC
EagI 1668 CGGCCG C^GGCCG
4301 CGGCCG
EclXI 1668 CGGCCG C^GGCCG
4301 CGGCCG
Eco52I 1668 CGGCCG C^GGCCG
4301 CGGCCG
EcoT22I 1238 ATGCAT ATGCA^T
5455 ATGCAT
KspI 426 CCGCGG CCGC^GG
6420 CCGCGG
MfeI 2232 CAATTG C^AATTG
5844 CAATTG
MroI 4604 TCCGGA T^CCGGA
5325 TCCGGA
MunI 2232 CAATTG C^AATTG
5844 CAATTG
NsiI 1238 ATGCAT ATGCA^T
5455 ATGCAT
PinAI 461 ACCGGT A^CCGGT
980 ACCGGT
SacII 426 CCGCGG CCGC^GG
6420 CCGCGG
SexAI 1799 ACCAGGT A^CC[AT]GGT
3149 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 148 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BssSI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI