Restriction map for
pF1KA0240
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AhdI Alw44I Aor51HI
ApaLI AscI Asp718I AspEI AviII
AvrII BamHI BbeI BcgI BclI
BfrI BglI BlnI BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BspEI
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstUI BstXI BstZ17I CfoI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PmeI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AgeI 2599 ACCGGT A^CCGGT
ApaI 211 GGGCCC GGGCC^C
AspI 1607 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2816 GGCACC G^G[CT][GA]CC
BbrPI 2209 CACGTG CAC^GTG
BbvCI 1474 CCTCAGC CCTCAGC(-5/-2)
BciVI 3224 GTATCC GTATCC(6/5)
BglII 2997 AGATCT A^GATCT
BsmI 2692 GAATGC GAATGC(1/-1)
Bsp1407I 549 TGTACA T^GTACA
BspDI 2380 ATCGAT AT^CGAT
BspMI 2043 GCAGGT ACCTGC(4/8)
BsrDI 92 GCAATG GCAATG(2/0)
BsrFI 2599 ACCGGT [GA]^CCGG[CT]
BsrGI 549 TGTACA T^GTACA
BssSI 18 CTCGTG CACGAG(-5/-1)
BstAPI 523 GCATCGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 2599 ACCGGT [GA]^CCGG[CT]
ClaI 2380 ATCGAT AT^CGAT
DrdI 1593 GACAAGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1804 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MslI 1074 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1169 CCATGG C^CATGG
PaeR7I 265 CTCGAG C^TCGAG
PflFI 1607 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1369 CCATATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2599 ACCGGT A^CCGGT
PmaCI 2209 CACGTG CAC^GTG
PmlI 2209 CACGTG CAC^GTG
SspBI 549 TGTACA T^GTACA
StuI 2074 AGGCCT AGG^CCT
Tth111I 1607 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1369 CCATATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 265 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 1779 GTCTAC GT^[AC][GT]AC
2443 GTAGAC
AlwNI 481 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
2191 CAGAGACTG
AseI 682 ATTAAT AT^TAAT
931 ATTAAT
AsnI 682 ATTAAT AT^TAAT
931 ATTAAT
Asp700I 1337 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2672 GAAACATTTC
AvaI 265 CTCGAG C^[CT]CG[GA]G
2420 CCCGAG
BalI 1302 TGGCCA TGG^CCA
2028 TGGCCA
BcnI 2803 CCCGG CC^[GC]GG
3165 CCCGG
BlpI 415 GCTCAGC GC^T[ACGT]AGC
1631 GCTCAGC
Bpu1102I 415 GCTCAGC GC^T[ACGT]AGC
1631 GCTCAGC
BsaAI 1009 CACGTA [CT]AC^GT[GA]
2209 CACGTG
BsaWI 2599 ACCGGT [AT]^CCGG[AT]
2819 ACCGGA
BseRI 275 CTCCTC GAGGAG(10/8)
1472 CTCCTC
BsgI 2041 GTGCAG GTGCAG(16/14)
2734 GTGCAG
BsoBI 265 CTCGAG C^[CT]CG[GA]G
2420 CCCGAG
BspLU11I 570 ACATGT A^CATGT
1073 ACATGT
CelII 415 GCTCAGC GC^T[ACGT]AGC
1631 GCTCAGC
DsaI 1169 CCATGG C^C[GA][CT]GG
3083 CCACGG
EcoRI 995 GAATTC G^AATTC
2934 GAATTC
FauI 722 GCGGG CCCGC(4/6)
3058 GCGGG
HgaI 483 GACGC GACGC(5/10)
504 GACGC
MluNI 1302 TGGCCA TGG^CCA
2028 TGGCCA
MscI 1302 TGGCCA TGG^CCA
2028 TGGCCA
NciI 2803 CCCGG CC^[GC]GG
3165 CCCGG
PpuMI 1605 AGGACCT [GA]G^G[AT]CC[CT]
2296 AGGACCC
PshBI 682 ATTAAT AT^TAAT
931 ATTAAT
XmnI 1337 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2672 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApoI AspHI AvaII
BanII BbsI BbvI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DraIII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
PshAI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII