Restriction map for
pF1KA0058
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# NO CUT ENZYMES ( 188 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI Aor51HI ApaI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstUI BstYI CfoI
Cfr10I ClaI CpoI DpnII DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII HphI KasI KpnI Ksp632I
KspI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MslI MspI MunI MvnI
NaeI NarI NciI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AccI 64 GTATAC GT^[AC][GT]AC
Alw44I 151 GTGCAC G^TGCAC
AlwNI 158 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 88 CCTCAGG CC^T[ACGT]AGG
ApaLI 151 GTGCAC G^TGCAC
Asp700I 462 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 283 GGTCC G^G[AT]CC
BanI 337 GGTGCC G^G[CT][GA]CC
BanII 130 GAGCTC G[GA]GC[CT]^C
BcgI 143 CGAGCTTTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 409 GCTCAGC GC^T[ACGT]AGC
BpmI 197 CTGGAG CTGGAG(16/14)
Bpu1102I 409 GCTCAGC GC^T[ACGT]AGC
BseRI 377 CTCCTC GAGGAG(10/8)
BsgI 43 GTGCAG GTGCAG(16/14)
Bst1107I 64 GTATAC GTA^TAC
BstF5I 391 GGATG GGATG(2/0)
BstXI 305 CCACAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 64 GTATAC GTA^TAC
Bsu36I 88 CCTCAGG CC^T[ACGT]AGG
CelII 409 GCTCAGC GC^T[ACGT]AGC
Eco81I 88 CCTCAGG CC^T[ACGT]AGG
FokI 391 GGATG GGATG(9/13)
HinfI 57 GAATC G^A[ACGT]TC
MaeII 437 ACGT A^CGT
MaeIII 445 GTAAC ^GT[ACGT]AC
MspA1I 451 CAGCGG C[AC]G^C[GT]G
SacI 130 GAGCTC GAGCT^C
TfiI 57 GAATC G^A[AT]TC
TspRI 308 CAGTG CA[GC]TG[ACGT][ACGT]^
XmnI 462 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AciI 188 CCGC CCGC(-3/-1)
453 GCGG
AspHI 130 GAGCTC G[AT]GC[AT]^C
151 GTGCAC
BmyI 130 GAGCTC G[AGT]GC[ACT]^C
151 GTGCAC
BsiHKAI 130 GAGCTC G[AT]GC[AT]^C
151 GTGCAC
BsiYI 225 CCAGTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
382 CCACCTCCTGG
BslI 225 CCAGTCTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
382 CCACCTCCTGG
BsmFI 172 GTCCC GGGAC(10/14)
284 GTCCC
Bsp1286I 130 GAGCTC G[AGT]GC[ACT]^C
151 GTGCAC
BspMI 117 ACCTGC ACCTGC(4/8)
334 GCAGGT
Cac8I 364 GCTGGC GC[ACGT]^[ACGT]GC
414 GCTTGC
DsaI 218 CCATGG C^C[GA][CT]GG
263 CCATGG
EcoT14I 218 CCATGG C^C[AT][AT]GG
263 CCATGG
HaeIII 222 GGCC GG^CC
242 GGCC
MwoI 112 GCTCCACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
409 GCTCAGCTTGC
NcoI 218 CCATGG C^CATGG
263 CCATGG
PflMI 225 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
382 CCACCTCCTGG
StyI 218 CCATGG C^C[AT][AT]GG
263 CCATGG
Van91I 225 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
382 CCACCTCCTGG
XcmI 254 CCACAATCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
305 CCACAGTGCTGGTGG
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BbvI BsaJI BsrI BssKI
BstNI Cfr13I DdeI EcoRII Fnu4HI
ItaI MboII MnlI MvaI NlaIII
NlaIV Sau96I ScrFI SfaNI TseI