Restriction map for
pF1KF0188
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# NO CUT ENZYMES ( 59 enzymes) #
##################################
AatII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
AspEI BbrPI BciVI BsaAI BsaI
BsiEI BsiWI BspDI BspLU11I BssHII
BstBI ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
FseI HpaI KspI MfeI MluI
MunI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacII SalI SbfI
SfiI SfuI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 3060 GTATAC GT^[AC][GT]AC
AclI 2161 AACGTT AA^CGTT
AflII 2814 CTTAAG C^TTAAG
Asp700I 3238 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 952 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1009 CCTAGG C^CTAGG
BbvCI 3438 CCTCAGC CCTCAGC(-5/-2)
BcgI 3524 CGAGGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 2814 CTTAAG C^TTAAG
BglI 164 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3020 AGATCT A^GATCT
BlnI 1009 CCTAGG C^CTAGG
BlpI 1181 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1181 GCTCAGC GC^T[ACGT]AGC
BsaBI 2976 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1902 GAATGC GAATGC(1/-1)
Bsp1407I 2636 TGTACA T^GTACA
BspHI 2918 TCATGA T^CATGA
BsrDI 974 GCAATG GCAATG(2/0)
BsrGI 2636 TGTACA T^GTACA
BssSI 712 CTCGTG CACGAG(-5/-1)
Bst1107I 3060 GTATAC GTA^TAC
BstEII 123 GGTGACC G^GT[ACGT]ACC
BstPI 123 GGTGACC G^GT[ACGT]ACC
BstZ17I 3060 GTATAC GTA^TAC
CelII 1181 GCTCAGC GC^T[ACGT]AGC
EcoO65I 123 GGTGACC G^GT[ACGT]ACC
EcoRV 3217 GATATC GAT^ATC
EcoT22I 1138 ATGCAT ATGCA^T
HincII 1356 GTTGAC GT[CT]^[GA]AC
HindII 1356 GTTGAC GT[CT]^[GA]AC
MamI 2976 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 3104 CATATG CA^TATG
NheI 2718 GCTAGC G^CTAGC
NsiI 1138 ATGCAT ATGCA^T
PaeR7I 556 CTCGAG C^TCGAG
PflFI 952 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2598 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2161 AACGTT AA^CGTT
RcaI 2918 TCATGA T^CATGA
ScaI 2873 AGTACT AGT^ACT
SexAI 2048 ACCAGGT A^CC[AT]GGT
SgrAI 2137 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 2636 TGTACA T^GTACA
Tth111I 952 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 556 CTCGAG C^TCGAG
XmnI 3238 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 852 GGTACC G^GTACC
2261 GGTACC
AccIII 323 TCCGGA T^CCGGA
3824 TCCGGA
ApaI 1019 GGGCCC GGGCC^C
1198 GGGCCC
Asp718I 852 GGTACC G^GTACC
2261 GGTACC
BamHI 334 GGATCC G^GATCC
1557 GGATCC
BbeI 1940 GGCGCC GGCGC^C
2893 GGCGCC
BclI 2303 TGATCA T^GATCA
3134 TGATCA
BsaWI 323 TCCGGA [AT]^CCGG[AT]
3824 TCCGGA
BseAI 323 TCCGGA T^CCGGA
3824 TCCGGA
BsmBI 1092 CGTCTC CGTCTC(1/5)
1746 GAGACG
BspEI 323 TCCGGA T^CCGGA
3824 TCCGGA
DraIII 1574 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2047 CACCAGGTG
EcoRI 371 GAATTC G^AATTC
1488 GAATTC
FbaI 2303 TGATCA T^GATCA
3134 TGATCA
HindIII 211 AAGCTT A^AGCTT
895 AAGCTT
KasI 1940 GGCGCC G^GCGCC
2893 GGCGCC
KpnI 852 GGTACC GGTAC^C
2261 GGTACC
MroI 323 TCCGGA T^CCGGA
3824 TCCGGA
NarI 1940 GGCGCC GG^CGCC
2893 GGCGCC
NcoI 275 CCATGG C^CATGG
2354 CCATGG
NspI 505 GCATGT [GA]CATG^[CT]
3356 ACATGC
SacI 789 GAGCTC GAGCT^C
2905 GAGCTC
SfoI 1940 GGCGCC GGC^GCC
2893 GGCGCC
SmaI 1438 CCCGGG CCC^GGG
2889 CCCGGG
StuI 1756 AGGCCT AGG^CCT
3536 AGGCCT
XcmI 275 CCATGGTCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3113 CCATGAAGAATGTGG
XmaCI 1438 CCCGGG C^CCGGG
2889 CCCGGG
XmaI 1438 CCCGGG C^CCGGG
2889 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaI AvaII AviII BalI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII