Restriction map for
pF1KF0107
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AgeI AhdI AlwNI AocI Aor51HI
ApaI AscI Asp718I AspEI AspI
BamHI BbeI BbrPI BcgI BciVI
BglI BsaAI BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BssHII Bst1107I BstEII BstPI BstUI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I FseI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NarI NheI
NotI NruI PacI PflFI PinAI
PmaCI PmeI PmlI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I StuI SwaI Tth111I
XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AclI 3430 AACGTT AA^CGTT
AseI 199 ATTAAT AT^TAAT
AsnI 199 ATTAAT AT^TAAT
Asp700I 3879 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1188 CTCGAG C^[CT]CG[GA]G
AviII 3199 TGCGCA TGC^GCA
AvrII 2856 CCTAGG C^CTAGG
BbvCI 387 CCTCAGC CCTCAGC(-5/-2)
BlnI 2856 CCTAGG C^CTAGG
BsoBI 1188 CTCGAG C^[CT]CG[GA]G
BspLU11I 2876 ACATGT A^CATGT
BsrFI 3280 GCCGGC [GA]^CCGG[CT]
Cfr10I 3280 GCCGGC [GA]^CCGG[CT]
EcoNI 2175 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 3199 TGCGCA TGC^GCA
HaeII 3304 AGCGCC [GA]GCGC^[CT]
NaeI 3280 GCCGGC GCC^GGC
NgoMIV 3280 GCCGGC G^CCGGC
PaeR7I 1188 CTCGAG C^TCGAG
PflMI 1639 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1390 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 199 ATTAAT AT^TAAT
Psp1406I 3430 AACGTT AA^CGTT
SphI 3074 GCATGC GCATG^C
SspI 1707 AATATT AAT^ATT
Van91I 1639 CCAGTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1188 CTCGAG C^TCGAG
XmnI 3879 GAAGTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 850 GTCTAC GT^[AC][GT]AC
1426 GTCTAC
BalI 256 TGGCCA TGG^CCA
1829 TGGCCA
BanI 2377 GGCACC G^G[CT][GA]CC
3760 GGCACC
BclI 558 TGATCA T^GATCA
2063 TGATCA
BglII 3234 AGATCT A^GATCT
3851 AGATCT
BlpI 174 GCTGAGC GC^T[ACGT]AGC
2767 GCTGAGC
Bpu1102I 174 GCTGAGC GC^T[ACGT]AGC
2767 GCTGAGC
BsaWI 697 TCCGGT [AT]^CCGG[AT]
1002 ACCGGA
BspHI 669 TCATGA T^CATGA
887 TCATGA
BssSI 859 CACGAG CACGAG(-5/-1)
3490 CTCGTG
BstBI 2503 TTCGAA TT^CGAA
3745 TTCGAA
CelII 174 GCTGAGC GC^T[ACGT]AGC
2767 GCTGAGC
DrdI 582 GACTCACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2194 GACTCAAAAGTC
EaeI 256 TGGCCA [CT]^GGCC[GA]
1829 TGGCCA
EcoRV 181 GATATC GAT^ATC
3250 GATATC
EcoT22I 1532 ATGCAT ATGCA^T
3588 ATGCAT
FbaI 558 TGATCA T^GATCA
2063 TGATCA
MluNI 256 TGGCCA TGG^CCA
1829 TGGCCA
MscI 256 TGGCCA TGG^CCA
1829 TGGCCA
NdeI 2842 CATATG CA^TATG
3266 CATATG
NsiI 1532 ATGCAT ATGCA^T
3588 ATGCAT
NspV 2503 TTCGAA TT^CGAA
3745 TTCGAA
PpuMI 1331 AGGTCCT [GA]G^G[AT]CC[CT]
1388 AGGACCC
RcaI 669 TCATGA T^CATGA
887 TCATGA
SapI 1585 GAAGAGC GCTCTTC(1/4)
864 GCTCTTC
SfuI 2503 TTCGAA TT^CGAA
3745 TTCGAA
XcmI 1082 CCAACCTCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3227 CCAAGCAAGATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI Alw44I AlwI ApaLI ApoI
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII