Restriction map for
pF1KSDB0025
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AvaI
AviII AvrII BbeI BbvCI BcgI
BfaI BfrI BglII BlnI BpmI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspEI BspLU11I BsrBI BssHII
Bst1107I BstAPI BstBI BstZ17I CpoI
EagI EarI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HpaI KasI KpnI Ksp632I
KspI MaeI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp509I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 79 GACGTC GACGT^C
AflIII 740 ACGTGT A^C[GA][CT]GT
AhdI 714 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1498 GTGCAC G^TGCAC
AlwNI 1403 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1389 CCTGAGG CC^T[ACGT]AGG
ApaLI 1498 GTGCAC G^TGCAC
AspEI 714 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1405 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 941 TGGCCA TGG^CCA
BanII 121 GAGCTC G[GA]GC[CT]^C
BciVI 1161 GTATCC GTATCC(6/5)
BclI 287 TGATCA T^GATCA
BglI 1601 GCCTCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1144 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1144 GCTAAGC GC^T[ACGT]AGC
BsaBI 27 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 222 GAATGC GAATGC(1/-1)
Bsp1407I 497 TGTACA T^GTACA
BspDI 1618 ATCGAT AT^CGAT
BsrDI 702 CATTGC GCAATG(2/0)
BsrGI 497 TGTACA T^GTACA
BssSI 70 CACGAG CACGAG(-5/-1)
BstEII 1311 GGTCACC G^GT[ACGT]ACC
BstPI 1311 GGTCACC G^GT[ACGT]ACC
BstXI 227 CCACGTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1389 CCTGAGG CC^T[ACGT]AGG
CelII 1144 GCTAAGC GC^T[ACGT]AGC
ClaI 1618 ATCGAT AT^CGAT
DraI 1443 TTTAAA TTT^AAA
DrdI 361 GACCCCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 714 GACTCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1389 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1311 GGTCACC G^GT[ACGT]ACC
FbaI 287 TGATCA T^GATCA
HindIII 1147 AAGCTT A^AGCTT
MamI 27 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 941 TGGCCA TGG^CCA
MscI 941 TGGCCA TGG^CCA
MseI 1444 TTAA T^TAA
MspA1I 773 CAGCTG C[AC]G^C[GT]G
NcoI 249 CCATGG C^CATGG
PflFI 1405 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 105 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 714 GACTC GAGTC(4/5)
PvuII 773 CAGCTG CAG^CTG
SacI 121 GAGCTC GAGCT^C
SspBI 497 TGTACA T^GTACA
Tru9I 1444 TTAA T^TAA
Tth111I 1405 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 105 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 82 GTCTAC GT^[AC][GT]AC
1411 GTCTAC
AcyI 79 GACGTC G[GA]^CG[CT]C
1348 GGCGTC
AfaI 498 GTAC GT^AC
522 GTAC
AspHI 121 GAGCTC G[AT]GC[AT]^C
1498 GTGCAC
BamHI 26 GGATCC G^GATCC
1652 GGATCC
BbrPI 228 CACGTG CAC^GTG
391 CACGTG
BsaAI 228 CACGTG [CT]AC^GT[GA]
391 CACGTG
BsaHI 79 GACGTC G[GA]^CG[CT]C
1348 GGCGTC
BsaI 316 GAGACC GGTCTC(1/5)
808 GAGACC
BsiHKAI 121 GAGCTC G[AT]GC[AT]^C
1498 GTGCAC
BspMI 108 GCAGGT ACCTGC(4/8)
1251 GCAGGT
BsrFI 977 ACCGGC [GA]^CCGG[CT]
1372 ACCGGC
Cfr10I 977 ACCGGC [GA]^CCGG[CT]
1372 ACCGGC
DraIII 402 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1297 CACGGCGTG
DsaI 249 CCATGG C^C[GA][CT]GG
1296 CCACGG
EcoNI 30 CCTCATCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1663 CCTCCCGGAGG
Hin1I 79 GACGTC G[GA]^CG[CT]C
1348 GGCGTC
HincII 460 GTCAAC GT[CT]^[GA]AC
736 GTCAAC
HindII 460 GTCAAC GT[CT]^[GA]AC
736 GTCAAC
MslI 228 CACGTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
279 CACCACCATG
PmaCI 228 CACGTG CAC^GTG
391 CACGTG
PmlI 228 CACGTG CAC^GTG
391 CACGTG
RsaI 498 GTAC GT^AC
522 GTAC
SexAI 403 ACCTGGT A^CC[AT]GGT
731 ACCTGGT
TfiI 644 GAATC G^A[AT]TC
1588 GAATC
XcmI 158 CCACAGGAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
570 CCATGCTGAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanI BbsI BbvI BcnI BmyI
BpuAI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PpuMI RcaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XhoII