Restriction map for
pF1KSDA1934
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI BamHI BanI BbeI BbrPI
BbvCI BcgI BclI BfrI BlpI
Bpu1102I BsaBI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 101 AAATTC [GA]^AATT[CT]
AcyI 685 GACGCC G[GA]^CG[CT]C
AfaI 465 GTAC GT^AC
Alw44I 820 GTGCAC G^TGCAC
ApaLI 820 GTGCAC G^TGCAC
ApoI 101 AAATTC [GA]^AATT[CT]
AspI 211 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 881 CTCGGG C^[CT]CG[GA]G
AvrII 153 CCTAGG C^CTAGG
BciVI 1230 GTATCC GTATCC(6/5)
BcnI 1044 CCGGG CC^[GC]GG
BglI 558 GCCCATATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 698 AGATCT A^GATCT
BlnI 153 CCTAGG C^CTAGG
BsaHI 685 GACGCC G[GA]^CG[CT]C
BsaI 517 GAGACC GGTCTC(1/5)
BsaWI 1054 TCCGGT [AT]^CCGG[AT]
BseRI 1285 GAGGAG GAGGAG(10/8)
BsoBI 881 CTCGGG C^[CT]CG[GA]G
BspMI 540 GCAGGT ACCTGC(4/8)
EcoNI 502 CCTAGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 685 GACGC GACGC(5/10)
Hin1I 685 GACGCC G[GA]^CG[CT]C
MseI 961 TTAA T^TAA
NciI 1044 CCGGG CC^[GC]GG
NdeI 561 CATATG CA^TATG
PflFI 211 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1328 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 149 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 465 GTAC GT^AC
SacI 919 GAGCTC GAGCT^C
TaqI 975 TCGA T^CGA
TfiI 858 GATTC G^A[AT]TC
Tru9I 961 TTAA T^TAA
Tth111I 211 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 975 TCGA T^CGA
Van91I 1328 CCAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 311 GTAGAC GT^[AC][GT]AC
1058 GTAGAC
AlwI 428 GATCC GGATC(4/5)
927 GGATC
AlwNI 130 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1304 CAGGGGCTG
AocI 903 CCTCAGG CC^T[ACGT]AGG
1093 CCTCAGG
Asp700I 318 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
491 GAACTGCTTC
AvaII 45 GGACC G^G[AT]CC
150 GGTCC
AviII 139 TGCGCA TGC^GCA
842 TGCGCA
BalI 359 TGGCCA TGG^CCA
1218 TGGCCA
BsaAI 611 TACGTG [CT]AC^GT[GA]
1227 CACGTA
BsgI 1088 CTGCAC GTGCAG(16/14)
1105 GTGCAG
BstYI 427 AGATCC [GA]^GATC[CT]
698 AGATCT
Bsu36I 903 CCTCAGG CC^T[ACGT]AGG
1093 CCTCAGG
DsaI 80 CCATGG C^C[GA][CT]GG
1215 CCATGG
EaeI 359 TGGCCA [CT]^GGCC[GA]
1218 TGGCCA
Eco81I 903 CCTCAGG CC^T[ACGT]AGG
1093 CCTCAGG
FauI 898 CCCGC CCCGC(4/6)
636 GCGGG
FspI 139 TGCGCA TGC^GCA
842 TGCGCA
HapII 1044 CCGG C^CGG
1055 CCGG
HpaII 1044 CCGG C^CGG
1055 CCGG
MaeII 612 ACGT A^CGT
1228 ACGT
MflI 427 AGATCC [GA]^GATC[CT]
698 AGATCT
MluNI 359 TGGCCA TGG^CCA
1218 TGGCCA
MscI 359 TGGCCA TGG^CCA
1218 TGGCCA
MspI 1044 CCGG C^CGG
1055 CCGG
NcoI 80 CCATGG C^CATGG
1215 CCATGG
PleI 658 GACTC GAGTC(4/5)
1251 GACTC
SfaNI 403 GATGC GCATC(5/9)
74 GCATC
Tsp45I 239 GTCAC ^GT[GC]AC
544 GTCAC
XhoII 427 AGATCC [GA]^GATC[CT]
698 AGATCT
XmnI 318 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
491 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AspHI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsiHKAI BsiYI BslI BsmAI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HhaI
HinP1I HinfI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
StyI TseI Tsp509I TspRI