Restriction map for
pF1KSDA1734
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# NO CUT ENZYMES ( 65 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI ApoI AscI AseI AsnI
AviII BbrPI BciVI BfrI BsaBI
BseAI BsiWI BspDI BspEI BspHI
BspLU11I BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
MamI MfeI MluI MroI MunI
NdeI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI ScaI SfiI SfuI
SnaBI SpeI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 377 GGTACC G^GTACC
AhdI 190 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1052 AGCGCT AGC^GCT
ApaI 2970 GGGCCC GGGCC^C
Asp718I 377 GGTACC G^GTACC
AspEI 190 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 996 CCTAGG C^CTAGG
BamHI 6 GGATCC G^GATCC
BclI 2324 TGATCA T^GATCA
BglII 812 AGATCT A^GATCT
BlnI 996 CCTAGG C^CTAGG
BsaAI 361 TACGTG [CT]AC^GT[GA]
BsaWI 3722 ACCGGA [AT]^CCGG[AT]
BsiEI 1839 CGGCCG CG[GA][CT]^CG
BsmI 1693 GAATGC GAATGC(1/-1)
Bsp1407I 2120 TGTACA T^GTACA
BsrGI 2120 TGTACA T^GTACA
EagI 1839 CGGCCG C^GGCCG
Eam1105I 190 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1839 CGGCCG C^GGCCG
Eco47III 1052 AGCGCT AGC^GCT
Eco52I 1839 CGGCCG C^GGCCG
FbaI 2324 TGATCA T^GATCA
KpnI 377 GGTACC GGTAC^C
MslI 2478 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 3631 GCTAGC G^CTAGC
PaeR7I 1604 CTCGAG C^TCGAG
PpuMI 569 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 745 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 291 GAGCTC GAGCT^C
SbfI 3267 CCTGCAGG CCTGCA^GG
SexAI 191 ACCTGGT A^CC[AT]GGT
SgrAI 104 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1220 CCCGGG CCC^GGG
SphI 3851 GCATGC GCATG^C
Sse8387I 3267 CCTGCAGG CCTGCA^GG
SspBI 2120 TGTACA T^GTACA
Tsp509I 2829 AATT ^AATT
XhoI 1604 CTCGAG C^TCGAG
XmaCI 1220 CCCGGG C^CCGGG
XmaI 1220 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccI 637 GTCTAC GT^[AC][GT]AC
1807 GTCTAC
Asp700I 1857 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2856 GAAGTTCTTC
AspI 955 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1969 GACATCGTC
BcgI 1308 CGATGGCTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
491 GCACGGTGATCG
BlpI 855 GCTCAGC GC^T[ACGT]AGC
1033 GCTCAGC
Bpu1102I 855 GCTCAGC GC^T[ACGT]AGC
1033 GCTCAGC
BsrDI 609 CATTGC GCAATG(2/0)
1932 CATTGC
BstEII 1479 GGTGACC G^GT[ACGT]ACC
1644 GGTGACC
BstPI 1479 GGTGACC G^GT[ACGT]ACC
1644 GGTGACC
CelII 855 GCTCAGC GC^T[ACGT]AGC
1033 GCTCAGC
DraIII 1209 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3686 CACCCAGTG
EcoNI 3180 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3837 CCTGCTAGAGG
EcoO65I 1479 GGTGACC G^GT[ACGT]ACC
1644 GGTGACC
HincII 505 GTCAAC GT[CT]^[GA]AC
559 GTCAAC
HindII 505 GTCAAC GT[CT]^[GA]AC
559 GTCAAC
KspI 285 CCGCGG CCGC^GG
354 CCGCGG
MseI 1567 TTAA T^TAA
2822 TTAA
PflFI 955 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1969 GACATCGTC
PflMI 1227 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3615 CCAGAGCCTGG
PstI 574 CTGCAG CTGCA^G
3268 CTGCAG
SacII 285 CCGCGG CCGC^GG
354 CCGCGG
StuI 3264 AGGCCT AGG^CCT
3581 AGGCCT
Tru9I 1567 TTAA T^TAA
2822 TTAA
Tth111I 955 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1969 GACATCGTC
Van91I 1227 CCATTCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3615 CCAGAGCCTGG
XmnI 1857 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2856 GAAGTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI AocI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbeI BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NaeI NarI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV NspI PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII