Restriction map for
pF1KSDA1730
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccII AciI AclI
AflII AgeI AocI ApaI AscI
Asp718I AspI AviII AvrII BanI
BbeI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaI BsiEI BsiWI
BsmFI Bsp1407I BspDI BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DrdI EagI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoT22I FauI FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI MvnI NarI NciI
NdeI NheI NotI NruI NsiI
NspV PacI PflFI PinAI PmeI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tth111I XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AccI 1595 GTAGAC GT^[AC][GT]AC
AccIII 293 TCCGGA T^CCGGA
AcyI 36 GGCGTC G[GA]^CG[CT]C
AflIII 2025 ACATGT A^C[GA][CT]GT
AhdI 1851 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1556 GTGCAC G^TGCAC
Aor51HI 464 AGCGCT AGC^GCT
ApaLI 1556 GTGCAC G^TGCAC
AseI 1731 ATTAAT AT^TAAT
AsnI 1731 ATTAAT AT^TAAT
AspEI 1851 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1468 GAGCTC G[GA]GC[CT]^C
BbrPI 832 CACGTG CAC^GTG
BbsI 529 GAAGAC GAAGAC(2/6)
BglII 275 AGATCT A^GATCT
BpuAI 529 GAAGAC GAAGAC(2/6)
BsaAI 832 CACGTG [CT]AC^GT[GA]
BsaHI 36 GGCGTC G[GA]^CG[CT]C
BseAI 293 TCCGGA T^CCGGA
BseRI 31 GAGGAG GAGGAG(10/8)
BsgI 1451 CTGCAC GTGCAG(16/14)
BsmBI 38 CGTCTC CGTCTC(1/5)
BspEI 293 TCCGGA T^CCGGA
BspLU11I 2025 ACATGT A^CATGT
BsrDI 551 CATTGC GCAATG(2/0)
DraII 1945 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 1851 GACATTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 464 AGCGCT AGC^GCT
EcoO109I 1945 AGGTCCT [GA]G^G[ACGT]CC[CT]
HgaI 37 GCGTC GACGC(5/10)
Hin1I 36 GGCGTC G[GA]^CG[CT]C
MroI 293 TCCGGA T^CCGGA
MslI 336 CACAGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1805 CAGCTG C[AC]G^C[GT]G
PaeR7I 448 CTCGAG C^TCGAG
PmaCI 832 CACGTG CAC^GTG
PmlI 832 CACGTG CAC^GTG
PpuMI 1945 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 629 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1731 ATTAAT AT^TAAT
PvuII 1805 CAGCTG CAG^CTG
SacI 1468 GAGCTC GAGCT^C
XbaI 1641 TCTAGA T^CTAGA
XhoI 448 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AfaI 1463 GTAC GT^AC
1711 GTAC
AlwNI 415 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
682 CAGATACTG
Asp700I 297 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1246 GAACATTTTC
AspHI 1468 GAGCTC G[AT]GC[AT]^C
1556 GTGCAC
AvaI 4 CCCGAG C^[CT]CG[GA]G
448 CTCGAG
BalI 1912 TGGCCA TGG^CCA
2147 TGGCCA
BmyI 1468 GAGCTC G[AGT]GC[ACT]^C
1556 GTGCAC
BsaWI 293 TCCGGA [AT]^CCGG[AT]
1304 ACCGGA
BsiHKAI 1468 GAGCTC G[AT]GC[AT]^C
1556 GTGCAC
BsoBI 4 CCCGAG C^[CT]CG[GA]G
448 CTCGAG
Bsp1286I 1468 GAGCTC G[AGT]GC[ACT]^C
1556 GTGCAC
BspHI 1631 TCATGA T^CATGA
2109 TCATGA
BsrFI 16 GCCGGC [GA]^CCGG[CT]
476 GCCGGC
Cfr10I 16 GCCGGC [GA]^CCGG[CT]
476 GCCGGC
DraI 663 TTTAAA TTT^AAA
1086 TTTAAA
DraIII 1774 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2173 CACATTGTG
EaeI 1912 TGGCCA [CT]^GGCC[GA]
2147 TGGCCA
HaeII 464 AGCGCT [GA]GCGC^[CT]
1150 AGCGCC
MaeII 833 ACGT A^CGT
870 ACGT
MluNI 1912 TGGCCA TGG^CCA
2147 TGGCCA
MscI 1912 TGGCCA TGG^CCA
2147 TGGCCA
NaeI 16 GCCGGC GCC^GGC
476 GCCGGC
NgoMIV 16 GCCGGC G^CCGGC
476 GCCGGC
RcaI 1631 TCATGA T^CATGA
2109 TCATGA
RsaI 1463 GTAC GT^AC
1711 GTAC
SapI 763 GCTCTTC GCTCTTC(1/4)
1470 GCTCTTC
SphI 878 GCATGC GCATG^C
2060 GCATGC
XmnI 297 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1246 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BamHI BbvI BfaI BpmI BsaJI
BsiYI BslI BsmAI BsmI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EarI EcoRI EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII