Restriction map for
pF1KSDA1623
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# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BciVI BfrI
BglII BlnI BsaBI BsiWI BspDI
BspLU11I BsrDI BssHII BstBI BstEII
BstPI ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoT22I FseI HpaI KpnI
MamI MfeI MunI NdeI NotI
NruI NsiI NspI NspV PacI
PflFI PmeI PshAI PshBI Psp1406I
PvuI RsrII ScaI SfuI SgrAI
SnaBI SpeI SphI SspI SwaI
Tth111I XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccIII 94 TCCGGA T^CCGGA
AgeI 2138 ACCGGT A^CCGGT
Alw44I 2361 GTGCAC G^TGCAC
AocI 1195 CCTAAGG CC^T[ACGT]AGG
ApaLI 2361 GTGCAC G^TGCAC
AviII 380 TGCGCA TGC^GCA
BamHI 3618 GGATCC G^GATCC
BcgI 132 GCAGGCTGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 2877 GCCTGAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 94 TCCGGA T^CCGGA
BsmI 269 GCATTC GAATGC(1/-1)
Bsp1407I 806 TGTACA T^GTACA
BspEI 94 TCCGGA T^CCGGA
BsrBI 1687 GAGCGG CCGCTC(-3/-3)
BsrGI 806 TGTACA T^GTACA
Bst1107I 778 GTATAC GTA^TAC
BstAPI 519 GCAGAATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1925 CCACTATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 778 GTATAC GTA^TAC
Bsu36I 1195 CCTAAGG CC^T[ACGT]AGG
Eco81I 1195 CCTAAGG CC^T[ACGT]AGG
EcoRV 2596 GATATC GAT^ATC
FspI 380 TGCGCA TGC^GCA
HindIII 109 AAGCTT A^AGCTT
MluI 310 ACGCGT A^CGCGT
MroI 94 TCCGGA T^CCGGA
NaeI 3436 GCCGGC GCC^GGC
NgoMIV 3436 GCCGGC G^CCGGC
NheI 1248 GCTAGC G^CTAGC
PinAI 2138 ACCGGT A^CCGGT
SacI 277 GAGCTC GAGCT^C
SalI 882 GTCGAC G^TCGAC
SbfI 129 CCTGCAGG CCTGCA^GG
SexAI 146 ACCTGGT A^CC[AT]GGT
SfiI 2876 GGCCTGAACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 129 CCTGCAGG CCTGCA^GG
SspBI 806 TGTACA T^GTACA
XbaI 699 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 778 GTATAC GT^[AC][GT]AC
882 GTCGAC
AcsI 522 GAATTT [GA]^AATT[CT]
2959 AAATTC
Aor51HI 1391 AGCGCT AGC^GCT
3336 AGCGCT
ApoI 522 GAATTT [GA]^AATT[CT]
2959 AAATTC
BbrPI 602 CACGTG CAC^GTG
2581 CACGTG
BclI 825 TGATCA T^GATCA
1421 TGATCA
BsaWI 94 TCCGGA [AT]^CCGG[AT]
2138 ACCGGT
BsgI 673 GTGCAG GTGCAG(16/14)
2257 GTGCAG
BsiEI 221 CGACCG CG[GA][CT]^CG
2140 CGGTCG
BspHI 659 TCATGA T^CATGA
2924 TCATGA
BspMI 618 GCAGGT ACCTGC(4/8)
1628 GCAGGT
BssSI 634 CACGAG CACGAG(-5/-1)
2665 CACGAG
BstYI 2336 AGATCC [GA]^GATC[CT]
3618 GGATCC
DraIII 553 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
3521 CACCCTGTG
Eco47III 1391 AGCGCT AGC^GCT
3336 AGCGCT
FbaI 825 TGATCA T^GATCA
1421 TGATCA
KspI 417 CCGCGG CCGC^GG
1488 CCGCGG
MflI 2336 AGATCC [GA]^GATC[CT]
3618 GGATCC
MseI 2467 TTAA T^TAA
2657 TTAA
NcoI 2432 CCATGG C^CATGG
3530 CCATGG
PaeR7I 2545 CTCGAG C^TCGAG
3625 CTCGAG
PleI 949 GACTC GAGTC(4/5)
3112 GACTC
PmaCI 602 CACGTG CAC^GTG
2581 CACGTG
PmlI 602 CACGTG CAC^GTG
2581 CACGTG
RcaI 659 TCATGA T^CATGA
2924 TCATGA
SacII 417 CCGCGG CCGC^GG
1488 CCGCGG
SmaI 2601 CCCGGG CCC^GGG
3179 CCCGGG
StuI 2651 AGGCCT AGG^CCT
2834 AGGCCT
Tru9I 2467 TTAA T^TAA
2657 TTAA
XcmI 2525 CCATCGTGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2978 CCACATCCTCACTGG
XhoI 2545 CTCGAG C^TCGAG
3625 CTCGAG
XhoII 2336 AGATCC [GA]^GATC[CT]
3618 GGATCC
XmaCI 2601 CCCGGG C^CCGGG
3179 CCCGGG
XmaI 2601 CCCGGG C^CCGGG
3179 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbsI BbvCI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV PflMI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I