Restriction map for
pF1KSDA1064
##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AgeI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BalI BbrPI BclI BfrI
BglII BlnI BsaAI BsaBI BsiWI
BsmI BspDI BspHI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DraIII DrdI Eco47III EcoRI EcoRV
FbaI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MluNI MscI MunI NheI NotI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SexAI SfuI SnaBI SpeI
Sse8387I SspI StuI SwaI Tth111I
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2481 GACGTC GACGT^C
AccIII 226 TCCGGA T^CCGGA
AflIII 1721 ACATGT A^C[GA][CT]GT
AhdI 2851 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3739 GTGCAC G^TGCAC
AlwNI 1702 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2310 CCTGAGG CC^T[ACGT]AGG
ApaLI 3739 GTGCAC G^TGCAC
AspEI 2851 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2315 GGATCC G^GATCC
BciVI 219 GGATAC GTATCC(6/5)
BfaI 395 CTAG C^TAG
BseAI 226 TCCGGA T^CCGGA
Bsp1407I 1724 TGTACA T^GTACA
BspEI 226 TCCGGA T^CCGGA
BspLU11I 1721 ACATGT A^CATGT
BsrDI 3527 GCAATG GCAATG(2/0)
BsrGI 1724 TGTACA T^GTACA
BstEII 451 GGTCACC G^GT[ACGT]ACC
BstPI 451 GGTCACC G^GT[ACGT]ACC
Bsu36I 2310 CCTGAGG CC^T[ACGT]AGG
DraI 3871 TTTAAA TTT^AAA
Eam1105I 2851 GACCCACGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2310 CCTGAGG CC^T[ACGT]AGG
EcoNI 2544 CCTCCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 451 GGTCACC G^GT[ACGT]ACC
EcoT22I 1882 ATGCAT ATGCA^T
FseI 2248 GGCCGGCC GGCCGG^CC
MaeI 395 CTAG C^TAG
MroI 226 TCCGGA T^CCGGA
MseI 3872 TTAA T^TAA
NcoI 1988 CCATGG C^CATGG
NdeI 482 CATATG CA^TATG
NruI 766 TCGCGA TCG^CGA
NsiI 1882 ATGCAT ATGCA^T
SapI 2164 GAAGAGC GCTCTTC(1/4)
SfiI 1993 GGCCCTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2265 CGCCGGTG C[GA]^CCGG[CT]G
SphI 516 GCATGC GCATG^C
SspBI 1724 TGTACA T^GTACA
Tru9I 3872 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BbsI 1081 GAAGAC GAAGAC(2/6)
2463 GAAGAC
BcgI 1264 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1187 GCAAGTACTTCG
BlpI 2514 GCTGAGC GC^T[ACGT]AGC
3426 GCTGAGC
Bpu1102I 2514 GCTGAGC GC^T[ACGT]AGC
3426 GCTGAGC
BpuAI 1081 GAAGAC GAAGAC(2/6)
2463 GAAGAC
BsgI 1209 CTGCAC GTGCAG(16/14)
3034 CTGCAC
BsmBI 896 GAGACG CGTCTC(1/5)
1417 GAGACG
BspMI 3746 ACCTGC ACCTGC(4/8)
1462 GCAGGT
BstXI 1353 CCACACCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2720 CCAGCCCTGTGG
BstYI 1736 AGATCC [GA]^GATC[CT]
2315 GGATCC
CelII 2514 GCTGAGC GC^T[ACGT]AGC
3426 GCTGAGC
HgaI 103 GACGC GACGC(5/10)
2977 GACGC
MaeII 2482 ACGT A^CGT
2894 ACGT
MflI 1736 AGATCC [GA]^GATC[CT]
2315 GGATCC
PflMI 2038 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3808 CCATTTGCTGG
SacI 1426 GAGCTC GAGCT^C
3537 GAGCTC
ScaI 914 AGTACT AGT^ACT
1190 AGTACT
Tsp509I 188 AATT ^AATT
1231 AATT
Van91I 2038 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3808 CCATTTGCTGG
XcmI 1769 CCACGGGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3011 CCATGCCCACCCTGG
XhoII 1736 AGATCC [GA]^GATC[CT]
2315 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BanI BanII BbeI BbvCI BbvI
BcnI BglI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeIII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I XmaCI XmaI