Restriction map for
pF1KSDA0990
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# NO CUT ENZYMES ( 78 enzymes) #
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Acc65I AclI AflII AgeI AhdI
Alw44I Aor51HI ApaLI AseI AsnI
Asp718I AspEI AviII AvrII BbvCI
BcgI BciVI BfaI BfrI BlnI
BsiWI BsmBI BsmI BspDI BsrDI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI DraI DraIII Eam1105I
Eco47III EcoO65I EcoT22I FseI FspI
HgaI HindIII HpaI KpnI KspI
MaeI MfeI MunI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI SacII SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1891 GACGTC GACGT^C
AccI 1894 GTCGAC GT^[AC][GT]AC
AccIII 776 TCCGGA T^CCGGA
AlwNI 2323 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 552 CCTGAGG CC^T[ACGT]AGG
ApaI 111 GGGCCC GGGCC^C
AscI 17 GGCGCGCC GG^CGCGCC
BalI 1478 TGGCCA TGG^CCA
BbeI 119 GGCGCC GGCGC^C
BbrPI 2085 CACGTG CAC^GTG
BsaAI 2085 CACGTG [CT]AC^GT[GA]
BsaBI 2254 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 533 GAGACC GGTCTC(1/5)
BsaWI 776 TCCGGA [AT]^CCGG[AT]
BseAI 776 TCCGGA T^CCGGA
BsiEI 12 CGGCCG CG[GA][CT]^CG
BspEI 776 TCCGGA T^CCGGA
BspLU11I 1634 ACATGT A^CATGT
BsrBI 107 GAGCGG CCGCTC(-3/-3)
BssSI 1699 CTCGTG CACGAG(-5/-1)
BstAPI 1797 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 552 CCTGAGG CC^T[ACGT]AGG
CpoI 145 CGGTCCG CG^G[AT]CCG
EagI 12 CGGCCG C^GGCCG
EclXI 12 CGGCCG C^GGCCG
Eco52I 12 CGGCCG C^GGCCG
Eco81I 552 CCTGAGG CC^T[ACGT]AGG
EcoRI 1915 GAATTC G^AATTC
EcoRV 969 GATATC GAT^ATC
HaeII 119 GGCGCC [GA]GCGC^[CT]
KasI 119 GGCGCC G^GCGCC
MamI 2254 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 1409 ACGCGT A^CGCGT
MluNI 1478 TGGCCA TGG^CCA
MroI 776 TCCGGA T^CCGGA
MscI 1478 TGGCCA TGG^CCA
NarI 119 GGCGCC GG^CGCC
PmaCI 2085 CACGTG CAC^GTG
PmlI 2085 CACGTG CAC^GTG
PpuMI 1055 AGGACCT [GA]G^G[AT]CC[CT]
PstI 292 CTGCAG CTGCA^G
RsrII 145 CGGTCCG CG^G[AT]CCG
SacI 976 GAGCTC GAGCT^C
SalI 1894 GTCGAC G^TCGAC
SapI 1878 GCTCTTC GCTCTTC(1/4)
SfoI 119 GGCGCC GGC^GCC
SmaI 86 CCCGGG CCC^GGG
StuI 597 AGGCCT AGG^CCT
Tsp45I 2024 GTGAC ^GT[GC]AC
XmaCI 86 CCCGGG C^CCGGG
XmaI 86 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 40 enzymes) #
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AcyI 119 GGCGCC G[GA]^CG[CT]C
1891 GACGTC
Asp700I 441 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1915 GAATTCCTTC
AspHI 976 GAGCTC G[AT]GC[AT]^C
1573 GAGCAC
AspI 1219 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1897 GACCTCGTC
BamHI 1504 GGATCC G^GATCC
1849 GGATCC
BbsI 1665 GAAGAC GAAGAC(2/6)
2196 GAAGAC
BclI 674 TGATCA T^GATCA
1244 TGATCA
BglI 131 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
172 GCCGGCGGGGC
BglII 870 AGATCT A^GATCT
2103 AGATCT
BlpI 27 GCTCAGC GC^T[ACGT]AGC
957 GCTGAGC
Bpu1102I 27 GCTCAGC GC^T[ACGT]AGC
957 GCTGAGC
BpuAI 1665 GAAGAC GAAGAC(2/6)
2196 GAAGAC
BsaHI 119 GGCGCC G[GA]^CG[CT]C
1891 GACGTC
BsgI 1000 CTGCAC GTGCAG(16/14)
793 GTGCAG
BsiHKAI 976 GAGCTC G[AT]GC[AT]^C
1573 GAGCAC
BsmAI 533 GAGAC GTCTC(1/5)
725 GTCTC
BsmFI 1393 GTCCC GGGAC(10/14)
1543 GTCCC
CelII 27 GCTCAGC GC^T[ACGT]AGC
957 GCTGAGC
DraII 663 GGGGCCT [GA]G^G[ACGT]CC[CT]
1055 AGGACCT
DrdI 1216 GACGACATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2307 GACCTATGGGTC
EcoNI 1082 CCTTCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1177 CCTCGAAGAGG
EcoO109I 663 GGGGCCT [GA]G^G[ACGT]CC[CT]
1055 AGGACCT
EcoT14I 1211 CCTTGG C^C[AT][AT]GG
2139 CCAAGG
FbaI 674 TGATCA T^GATCA
1244 TGATCA
Hin1I 119 GGCGCC G[GA]^CG[CT]C
1891 GACGTC
HincII 1162 GTTGAC GT[CT]^[GA]AC
1894 GTCGAC
HindII 1162 GTTGAC GT[CT]^[GA]AC
1894 GTCGAC
MaeIII 2024 GTGAC ^GT[ACGT]AC
2363 GTTAC
MseI 1775 TTAA T^TAA
1862 TTAA
NaeI 14 GCCGGC GCC^GGC
172 GCCGGC
NgoMIV 14 GCCGGC G^CCGGC
172 GCCGGC
PflFI 1219 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1897 GACCTCGTC
PflMI 474 CCACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1440 CCAGTTTGTGG
SmlI 360 CTCAAG C^T[CT][GA]AG
457 CTCAAG
StyI 1211 CCTTGG C^C[AT][AT]GG
2139 CCAAGG
Tru9I 1775 TTAA T^TAA
1862 TTAA
Tth111I 1219 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1897 GACCTCGTC
Van91I 474 CCACTACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1440 CCAGTTTGTGG
XcmI 608 CCACGGAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2318 CCACACAGCAGCTGG
XmnI 441 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1915 GAATTCCTTC
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# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApoI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsoBI Bsp1286I Bsp1407I BspHI BspMI
BsrFI BsrGI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PvuII RcaI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI TaqI TfiI TseI
Tsp509I TspRI TthHB8I XhoII