Restriction map for
pF1KSDA0979
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# NO CUT ENZYMES ( 109 enzymes) #
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AatII AccII AccIII AclI AcyI
AflIII AgeI AhdI Aor51HI ApaI
AscI AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I CelII Cfr10I ClaI CpoI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoT22I
FseI FspI Hin1I HindIII KasI
KspI MamI MluI MroI MvnI
NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI Psp1406I PstI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 609 GGTACC G^GTACC
AccI 532 GTAGAC GT^[AC][GT]AC
Alw44I 1831 GTGCAC G^TGCAC
ApaLI 1831 GTGCAC G^TGCAC
AseI 3081 ATTAAT AT^TAAT
AsnI 3081 ATTAAT AT^TAAT
Asp700I 1638 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 609 GGTACC G^GTACC
BalI 501 TGGCCA TGG^CCA
BanI 609 GGTACC G^G[CT][GA]CC
BglI 936 GCCACTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2385 AGATCT A^GATCT
BpmI 3962 CTCCAG CTGGAG(16/14)
BsaI 2221 GAGACC GGTCTC(1/5)
BsgI 2060 CTGCAC GTGCAG(16/14)
BsmFI 1230 GGGAC GGGAC(10/14)
BsmI 2740 GAATGC GAATGC(1/-1)
Bsp1407I 3194 TGTACA T^GTACA
BsrGI 3194 TGTACA T^GTACA
CfoI 864 GCGC GCG^C
DraII 2160 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 2162 GACCCCCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 501 TGGCCA [CT]^GGCC[GA]
EcoO109I 2160 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 970 GATATC GAT^ATC
EcoT14I 3159 CCAAGG C^C[AT][AT]GG
FauI 2907 GCGGG CCCGC(4/6)
HaeII 863 AGCGCC [GA]GCGC^[CT]
HgaI 4181 GACGC GACGC(5/10)
HhaI 864 GCGC GCG^C
HinP1I 864 GCGC G^CGC
HincII 3400 GTTAAC GT[CT]^[GA]AC
HindII 3400 GTTAAC GT[CT]^[GA]AC
HpaI 3400 GTTAAC GTT^AAC
KpnI 609 GGTACC GGTAC^C
MaeII 2628 ACGT A^CGT
MfeI 4088 CAATTG C^AATTG
MluNI 501 TGGCCA TGG^CCA
MscI 501 TGGCCA TGG^CCA
MunI 4088 CAATTG C^AATTG
NheI 1009 GCTAGC G^CTAGC
PpuMI 2160 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2463 GACAATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3081 ATTAAT AT^TAAT
SphI 2925 GCATGC GCATG^C
SspBI 3194 TGTACA T^GTACA
StuI 2784 AGGCCT AGG^CCT
StyI 3159 CCAAGG C^C[AT][AT]GG
XmnI 1638 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflII 1957 CTTAAG C^TTAAG
2550 CTTAAG
AocI 1084 CCTGAGG CC^T[ACGT]AGG
3793 CCTGAGG
BbsI 4127 GAAGAC GAAGAC(2/6)
3567 GTCTTC
BcnI 3270 CCCGG CC^[GC]GG
3580 CCGGG
BfrI 1957 CTTAAG C^TTAAG
2550 CTTAAG
BpuAI 4127 GAAGAC GAAGAC(2/6)
3567 GTCTTC
BsmBI 96 GAGACG CGTCTC(1/5)
3602 GAGACG
BstAPI 2053 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2821 GCAATCTGTGC
Bsu36I 1084 CCTGAGG CC^T[ACGT]AGG
3793 CCTGAGG
DraIII 3198 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4036 CACCGAGTG
Eco81I 1084 CCTGAGG CC^T[ACGT]AGG
3793 CCTGAGG
HapII 3271 CCGG C^CGG
3580 CCGG
HpaII 3271 CCGG C^CGG
3580 CCGG
MslI 1647 CACACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2575 CATAGTGATG
MspI 3271 CCGG C^CGG
3580 CCGG
NciI 3270 CCCGG CC^[GC]GG
3580 CCGGG
PleI 142 GACTC GAGTC(4/5)
3607 GACTC
SapI 2861 GAAGAGC GCTCTTC(1/4)
3910 GAAGAGC
ScaI 1692 AGTACT AGT^ACT
3238 AGTACT
SfcI 1409 CTACAG C^T[GA][CT]AG
4298 CTACAG
SwaI 834 ATTTAAAT ATTT^AAAT
1454 ATTTAAAT
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
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AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbvI BciVI BclI BfaI BmyI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspHI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII FbaI Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SmlI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII