Restriction map for
pF1KSDA0963
##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AclI AcsI AflII ApoI
AscI AseI AsnI Asp718I AviII
AvrII BclI BfrI BlnI BsaBI
BsiWI BspDI BspLU11I BsrDI Bst1107I
BstBI BstZ17I ClaI CpoI DraI
DraIII EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RsrII SalI SapI
SfiI SfuI SnaBI SpeI SphI
SspI SwaI TfiI Tru9I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 3550 GACGTC GACGT^C
AccIII 1334 TCCGGA T^CCGGA
AflIII 1544 ACGTGT A^C[GA][CT]GT
AgeI 2525 ACCGGT A^CCGGT
AhdI 334 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2343 CCTCAGG CC^T[ACGT]AGG
AspEI 334 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2032 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2077 GGATCC G^GATCC
BbrPI 2335 CACGTG CAC^GTG
BciVI 2776 GGATAC GTATCC(6/5)
BglII 3050 AGATCT A^GATCT
BseAI 1334 TCCGGA T^CCGGA
BspEI 1334 TCCGGA T^CCGGA
BstEII 3348 GGTCACC G^GT[ACGT]ACC
BstPI 3348 GGTCACC G^GT[ACGT]ACC
Bsu36I 2343 CCTCAGG CC^T[ACGT]AGG
Eam1105I 334 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2343 CCTCAGG CC^T[ACGT]AGG
EcoNI 384 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3348 GGTCACC G^GT[ACGT]ACC
HincII 2191 GTCAAC GT[CT]^[GA]AC
HindII 2191 GTCAAC GT[CT]^[GA]AC
MroI 1334 TCCGGA T^CCGGA
PflFI 2032 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2525 ACCGGT A^CCGGT
PmaCI 2335 CACGTG CAC^GTG
PmlI 2335 CACGTG CAC^GTG
PshAI 1159 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 3465 CCTGCAGG CCTGCA^GG
ScaI 2954 AGTACT AGT^ACT
SgrAI 53 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 934 CTCAAG C^T[CT][GA]AG
Sse8387I 3465 CCTGCAGG CCTGCA^GG
Tsp509I 1821 AATT ^AATT
Tth111I 2032 GACGTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1249 GTCTAC GT^[AC][GT]AC
1504 GTCTAC
Aor51HI 1622 AGCGCT AGC^GCT
2357 AGCGCT
Asp700I 120 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1542 GAACGTGTTC
BbvCI 2673 CCTCAGC CCTCAGC(-5/-2)
562 GCTGAGG
BfaI 754 CTAG C^TAG
1213 CTAG
BsaAI 613 TACGTG [CT]AC^GT[GA]
2335 CACGTG
BsaWI 1334 TCCGGA [AT]^CCGG[AT]
2525 ACCGGT
BsmBI 921 CGTCTC CGTCTC(1/5)
1788 CGTCTC
BsmI 1179 GAATGC GAATGC(1/-1)
2940 GAATGC
BspHI 173 TCATGA T^CATGA
2363 TCATGA
BssSI 784 CACGAG CACGAG(-5/-1)
2380 CTCGTG
EarI 1042 CTCTTC CTCTTC(1/4)
1368 GAAGAG
Eco47III 1622 AGCGCT AGC^GCT
2357 AGCGCT
Ksp632I 1042 CTCTTC CTCTTC(1/4)
1368 GAAGAG
MaeI 754 CTAG C^TAG
1213 CTAG
PflMI 398 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2232 CCAGCGGGTGG
RcaI 173 TCATGA T^CATGA
2363 TCATGA
SacI 177 GAGCTC GAGCT^C
2206 GAGCTC
SexAI 389 ACCAGGT A^CC[AT]GGT
2534 ACCAGGT
Van91I 398 CCACCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2232 CCAGCGGGTGG
XmnI 120 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1542 GAACGTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcgI
BcnI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EagI EclXI Eco52I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI KspI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SspBI StuI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XcmI XhoII XmaCI XmaI