Restriction map for
pF1KSDA0865
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# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BbrPI
BcgI BfrI BlnI BsaBI BseAI
BsiWI BspDI BspEI BspHI Bst1107I
BstBI BstXI BstZ17I ClaI CpoI
Eam1105I FseI FspI KpnI MamI
MroI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SexAI SfiI SfuI
SgrAI SnaBI SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 3332 GTCGAC GT^[AC][GT]AC
AgeI 1856 ACCGGT A^CCGGT
AocI 1382 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1268 AGCGCT AGC^GCT
BamHI 2860 GGATCC G^GATCC
BbsI 370 GAAGAC GAAGAC(2/6)
BbvCI 5361 GCTGAGG CCTCAGC(-5/-2)
BpuAI 370 GAAGAC GAAGAC(2/6)
BsaAI 3169 TACGTG [CT]AC^GT[GA]
BsmI 3606 GAATGC GAATGC(1/-1)
BspLU11I 1590 ACATGT A^CATGT
BsrBI 1158 GAGCGG CCGCTC(-3/-3)
BstEII 2494 GGTAACC G^GT[ACGT]ACC
BstPI 2494 GGTAACC G^GT[ACGT]ACC
Bsu36I 1382 CCTCAGG CC^T[ACGT]AGG
DrdI 340 GACAGAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1268 AGCGCT AGC^GCT
Eco81I 1382 CCTCAGG CC^T[ACGT]AGG
EcoO65I 2494 GGTAACC G^GT[ACGT]ACC
EcoRV 4095 GATATC GAT^ATC
HindIII 580 AAGCTT A^AGCTT
MfeI 1049 CAATTG C^AATTG
MluI 4421 ACGCGT A^CGCGT
MunI 1049 CAATTG C^AATTG
NaeI 4948 GCCGGC GCC^GGC
NgoMIV 4948 GCCGGC G^CCGGC
PflMI 2073 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1856 ACCGGT A^CCGGT
SalI 3332 GTCGAC G^TCGAC
SpeI 2806 ACTAGT A^CTAGT
StuI 4996 AGGCCT AGG^CCT
Van91I 2073 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2967 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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Alw44I 1850 GTGCAC G^TGCAC
3099 GTGCAC
ApaI 4408 GGGCCC GGGCC^C
4879 GGGCCC
ApaLI 1850 GTGCAC G^TGCAC
3099 GTGCAC
BciVI 3229 GGATAC GTATCC(6/5)
5275 GGATAC
BclI 756 TGATCA T^GATCA
2822 TGATCA
BglII 1286 AGATCT A^GATCT
3215 AGATCT
BsaWI 1705 ACCGGA [AT]^CCGG[AT]
1856 ACCGGT
BsmBI 267 CGTCTC CGTCTC(1/5)
3869 CGTCTC
BssSI 2437 CACGAG CACGAG(-5/-1)
847 CTCGTG
BstAPI 2482 GCATATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5448 GCAGATCCTGC
DraI 2155 TTTAAA TTT^AAA
2887 TTTAAA
DraIII 844 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
3832 CACTCGGTG
EcoRI 2268 GAATTC G^AATTC
5192 GAATTC
EcoT22I 826 ATGCAT ATGCA^T
1886 ATGCAT
FbaI 756 TGATCA T^GATCA
2822 TGATCA
HpaI 603 GTTAAC GTT^AAC
1321 GTTAAC
NsiI 826 ATGCAT ATGCA^T
1886 ATGCAT
PshAI 2900 GACATAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4491 GACCCCCGTC
SapI 979 GAAGAGC GCTCTTC(1/4)
1449 GCTCTTC
SbfI 1544 CCTGCAGG CCTGCA^GG
5391 CCTGCAGG
ScaI 761 AGTACT AGT^ACT
4622 AGTACT
Sse8387I 1544 CCTGCAGG CCTGCA^GG
5391 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbeI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrDI BsrFI BsrGI BsrI
BssHII BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I KspI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI SphI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI XmnI