Restriction map for
pF1KSDA0859
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AclI AcsI AcyI
AfaI AflII AgeI AhdI Aor51HI
ApaI ApoI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbeI BcgI BciVI BclI BfrI
BlnI BsaHI BsiWI BsmBI BsmI
Bsp1407I BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Cfr10I DraI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII Hin1I
HpaI KasI KpnI KspI MfeI
MluI MslI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RsaI SacII SapI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
StyI SwaI TfiI XbaI XhoI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 1235 GTCGAC GT^[AC][GT]AC
AccII 884 CGCG CG^CG
AccIII 1148 TCCGGA T^CCGGA
AflIII 2054 ACACGT A^C[GA][CT]GT
Alw44I 547 GTGCAC G^TGCAC
AlwNI 641 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 547 GTGCAC G^TGCAC
AspI 1189 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 364 GGCACC G^G[CT][GA]CC
BbrPI 853 CACGTG CAC^GTG
BbsI 399 GAAGAC GAAGAC(2/6)
BglII 662 AGATCT A^GATCT
BlpI 1054 GCTGAGC GC^T[ACGT]AGC
BpmI 706 CTGGAG CTGGAG(16/14)
Bpu1102I 1054 GCTGAGC GC^T[ACGT]AGC
BpuAI 399 GAAGAC GAAGAC(2/6)
BsaBI 903 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 928 GAGACC GGTCTC(1/5)
BsaWI 1148 TCCGGA [AT]^CCGG[AT]
BseAI 1148 TCCGGA T^CCGGA
BsiEI 459 CGGTCG CG[GA][CT]^CG
BspDI 1573 ATCGAT AT^CGAT
BspEI 1148 TCCGGA T^CCGGA
BspHI 623 TCATGA T^CATGA
BsrDI 1644 CATTGC GCAATG(2/0)
BstUI 884 CGCG CG^CG
BstXI 1602 CCAGTGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1054 GCTGAGC GC^T[ACGT]AGC
ClaI 1573 ATCGAT AT^CGAT
CpoI 1150 CGGACCG CG^G[AT]CCG
EarI 394 GAAGAG CTCTTC(1/4)
EcoNI 480 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 315 GACGC GACGC(5/10)
HindIII 80 AAGCTT A^AGCTT
Ksp632I 394 GAAGAG CTCTTC(1/4)
MamI 903 GATTTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1148 TCCGGA T^CCGGA
MseI 1802 TTAA T^TAA
MvnI 884 CGCG CG^CG
PflFI 1189 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 853 CACGTG CAC^GTG
PmlI 853 CACGTG CAC^GTG
RcaI 623 TCATGA T^CATGA
RsrII 1150 CGGACCG CG^G[AT]CCG
SacI 1990 GAGCTC GAGCT^C
SalI 1235 GTCGAC G^TCGAC
SbfI 447 CCTGCAGG CCTGCA^GG
SexAI 572 ACCAGGT A^CC[AT]GGT
Sse8387I 447 CCTGCAGG CCTGCA^GG
StuI 1500 AGGCCT AGG^CCT
Tru9I 1802 TTAA T^TAA
Tth111I 1189 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 916 CCTCAGG CC^T[ACGT]AGG
2025 CCTGAGG
Asp700I 54 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
78 GAAAGCTTTC
AvaI 524 CCCGGG C^[CT]CG[GA]G
1377 CCCGGG
BalI 557 TGGCCA TGG^CCA
1595 TGGCCA
BanII 1885 GGGCTC G[GA]GC[CT]^C
1990 GAGCTC
BbvCI 1248 CCTCAGC CCTCAGC(-5/-2)
430 GCTGAGG
BglI 696 GCCTGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1669 GCCAGCTTGGC
BsaAI 853 CACGTG [CT]AC^GT[GA]
2055 CACGTA
BseRI 1682 GAGGAG GAGGAG(10/8)
1685 GAGGAG
BsoBI 524 CCCGGG C^[CT]CG[GA]G
1377 CCCGGG
BspMI 450 GCAGGT ACCTGC(4/8)
1110 GCAGGT
Bsu36I 916 CCTCAGG CC^T[ACGT]AGG
2025 CCTGAGG
CfoI 690 GCGC GCG^C
777 GCGC
DrdI 1138 GACATTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2053 GACACGTATGTC
Eco81I 916 CCTCAGG CC^T[ACGT]AGG
2025 CCTGAGG
HhaI 690 GCGC GCG^C
777 GCGC
HinP1I 690 GCGC G^CGC
777 GCGC
MaeII 854 ACGT A^CGT
2056 ACGT
MluNI 557 TGGCCA TGG^CCA
1595 TGGCCA
MscI 557 TGGCCA TGG^CCA
1595 TGGCCA
SmaI 524 CCCGGG CCC^GGG
1377 CCCGGG
Tsp45I 1187 GTGAC ^GT[GC]AC
1625 GTGAC
Tsp509I 1923 AATT ^AATT
2091 AATT
XcmI 1583 CCATGTTGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1641 CCACATTGCAGATGG
XmaCI 524 CCCGGG C^CCGGG
1377 CCCGGG
XmaI 524 CCCGGG C^CCGGG
1377 CCCGGG
XmnI 54 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
78 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BcnI BfaI BmyI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DraIII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HincII HindII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
TspRI TthHB8I Van91I XhoII