Restriction map for
pF1KSDA0849
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflIII AgeI AhdI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BglI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsiWI BsmBI BsoBI BspDI BspHI
BspLU11I BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
CelII ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
EcoO65I FauI FbaI FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MluI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AciI 54 GCGG CCGC(-3/-1)
AflII 1689 CTTAAG C^TTAAG
Alw44I 2792 GTGCAC G^TGCAC
AocI 2035 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1658 AGCGCT AGC^GCT
ApaLI 2792 GTGCAC G^TGCAC
BanI 2232 GGCACC G^G[CT][GA]CC
BanII 1407 GGGCTC G[GA]GC[CT]^C
BfrI 1689 CTTAAG C^TTAAG
BsiEI 2667 CGATCG CG[GA][CT]^CG
Bsp1407I 2852 TGTACA T^GTACA
BsrBI 52 GAGCGG CCGCTC(-3/-3)
BsrFI 1638 GCCGGT [GA]^CCGG[CT]
BsrGI 2852 TGTACA T^GTACA
BstAPI 2549 GCATCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2035 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1638 GCCGGT [GA]^CCGG[CT]
Eco47III 1658 AGCGCT AGC^GCT
Eco81I 2035 CCTGAGG CC^T[ACGT]AGG
EcoNI 1572 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2041 GAATTC G^AATTC
EcoT22I 2813 ATGCAT ATGCA^T
HgaI 921 GACGC GACGC(5/10)
HincII 1078 GTTGAC GT[CT]^[GA]AC
HindII 1078 GTTGAC GT[CT]^[GA]AC
HindIII 777 AAGCTT A^AGCTT
MaeII 377 ACGT A^CGT
MfeI 312 CAATTG C^AATTG
MunI 312 CAATTG C^AATTG
NsiI 2813 ATGCAT ATGCA^T
NspI 2825 GCATGT [GA]CATG^[CT]
PflMI 2559 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1605 CTGCAG CTGCA^G
PvuI 2667 CGATCG CGAT^CG
ScaI 2732 AGTACT AGT^ACT
SspBI 2852 TGTACA T^GTACA
Van91I 2559 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 475 TCCGGA T^CCGGA
2406 TCCGGA
AlwNI 650 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1022 CAGACCCTG
AspHI 1489 GTGCTC G[AT]GC[AT]^C
2792 GTGCAC
AvaII 448 GGACC G^G[AT]CC
652 GGTCC
BalI 1290 TGGCCA TGG^CCA
1370 TGGCCA
BglII 406 AGATCT A^GATCT
1042 AGATCT
BsaWI 475 TCCGGA [AT]^CCGG[AT]
2406 TCCGGA
BseAI 475 TCCGGA T^CCGGA
2406 TCCGGA
BseRI 1202 CTCCTC GAGGAG(10/8)
319 GAGGAG
BsiHKAI 1489 GTGCTC G[AT]GC[AT]^C
2792 GTGCAC
BspEI 475 TCCGGA T^CCGGA
2406 TCCGGA
BsrDI 1631 CATTGC GCAATG(2/0)
2374 GCAATG
CfoI 1582 GCGC GCG^C
1659 GCGC
Cfr13I 448 GGACC G^G[ACGT]CC
652 GGTCC
DraII 447 AGGACCC [GA]G^G[ACGT]CC[CT]
651 AGGTCCT
EcoO109I 447 AGGACCC [GA]G^G[ACGT]CC[CT]
651 AGGTCCT
EcoRV 901 GATATC GAT^ATC
1161 GATATC
HaeII 1581 GGCGCT [GA]GCGC^[CT]
1658 AGCGCT
HhaI 1582 GCGC GCG^C
1659 GCGC
HinP1I 1582 GCGC G^CGC
1659 GCGC
MluNI 1290 TGGCCA TGG^CCA
1370 TGGCCA
MroI 475 TCCGGA T^CCGGA
2406 TCCGGA
MscI 1290 TGGCCA TGG^CCA
1370 TGGCCA
MspA1I 400 CAGCTG C[AC]G^C[GT]G
2417 CAGCTG
MwoI 11 GCTTATGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2549 GCATCCCTTGC
PpuMI 447 AGGACCC [GA]G^G[AT]CC[CT]
651 AGGTCCT
PvuII 400 CAGCTG CAG^CTG
2417 CAGCTG
Sau96I 448 GGACC G^G[ACGT]CC
652 GGTCC
TaqI 674 TCGA T^CGA
2262 TCGA
TthHB8I 674 TCGA T^CGA
2262 TCGA
XbaI 222 TCTAGA T^CTAGA
1398 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I BbsI BbvI BfaI BmyI
BpmI BpuAI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI NdeII NlaIII
NlaIV PleI RsaI SapI Sau3AI
ScrFI SfaNI SfcI SmlI SspI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI