Restriction map for
pF1KSDA0738
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# NO CUT ENZYMES ( 96 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AhdI Alw44I AlwNI Aor51HI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AviII AvrII BamHI
BbeI BbsI BbvCI BcgI BclI
BfrI BlnI BpuAI BsaBI BsaHI
BseAI BsiWI BspEI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI XmaCI
XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Acc65I 2183 GGTACC G^GTACC
AccI 997 GTCTAC GT^[AC][GT]AC
AccII 2096 CGCG CG^CG
AflIII 1373 ACATGT A^C[GA][CT]GT
AgeI 1784 ACCGGT A^CCGGT
AocI 1392 CCTGAGG CC^T[ACGT]AGG
Asp718I 2183 GGTACC G^GTACC
AspHI 2218 GAGCTC G[AT]GC[AT]^C
BbrPI 1818 CACGTG CAC^GTG
BciVI 1958 GTATCC GTATCC(6/5)
BglII 2156 AGATCT A^GATCT
BlpI 2107 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2107 GCTGAGC GC^T[ACGT]AGC
BsaAI 1818 CACGTG [CT]AC^GT[GA]
BsaI 1930 GAGACC GGTCTC(1/5)
BsaWI 1784 ACCGGT [AT]^CCGG[AT]
BsiEI 2021 CGACCG CG[GA][CT]^CG
BsiHKAI 2218 GAGCTC G[AT]GC[AT]^C
BspDI 1812 ATCGAT AT^CGAT
BspHI 422 TCATGA T^CATGA
BspLU11I 1373 ACATGT A^CATGT
BspMI 2332 ACCTGC ACCTGC(4/8)
BsrBI 1242 CCGCTC CCGCTC(-3/-3)
BsrDI 144 CATTGC GCAATG(2/0)
BsrFI 1784 ACCGGT [GA]^CCGG[CT]
BstUI 2096 CGCG CG^CG
Bsu36I 1392 CCTGAGG CC^T[ACGT]AGG
CelII 2107 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1784 ACCGGT [GA]^CCGG[CT]
ClaI 1812 ATCGAT AT^CGAT
DraI 577 TTTAAA TTT^AAA
DraIII 2623 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1392 CCTGAGG CC^T[ACGT]AGG
EcoRV 1412 GATATC GAT^ATC
HindIII 1756 AAGCTT A^AGCTT
KpnI 2183 GGTACC GGTAC^C
MvnI 2096 CGCG CG^CG
NcoI 759 CCATGG C^CATGG
PaeR7I 313 CTCGAG C^TCGAG
PflMI 2268 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1784 ACCGGT A^CCGGT
PmaCI 1818 CACGTG CAC^GTG
PmlI 1818 CACGTG CAC^GTG
PvuII 709 CAGCTG CAG^CTG
RcaI 422 TCATGA T^CATGA
SacI 2218 GAGCTC GAGCT^C
ScaI 1636 AGTACT AGT^ACT
SmlI 313 CTCGAG C^T[CT][GA]AG
StuI 2657 AGGCCT AGG^CCT
Van91I 2268 CCATGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2508 CCAGGAAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 313 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 603 GATCC GGATC(4/5)
1362 GATCC
AvaI 313 CTCGAG C^[CT]CG[GA]G
1433 CCCGAG
BfaI 715 CTAG C^TAG
742 CTAG
BglI 19 GCCTTCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
887 GCCGCAGAGGC
BsmBI 2539 CGTCTC CGTCTC(1/5)
2362 GAGACG
BsmI 2472 GAATGC GAATGC(1/-1)
2375 GCATTC
BsoBI 313 CTCGAG C^[CT]CG[GA]G
1433 CCCGAG
Bsp1407I 279 TGTACA T^GTACA
1547 TGTACA
BsrGI 279 TGTACA T^GTACA
1547 TGTACA
BssSI 567 CACGAG CACGAG(-5/-1)
520 CTCGTG
BstXI 1487 CCACAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1972 CCAGGGCCCTGG
EcoNI 1213 CCTAAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2442 CCTGAGCAAGG
EcoT22I 2173 ATGCAT ATGCA^T
2474 ATGCAT
HincII 1597 GTCAAC GT[CT]^[GA]AC
2587 GTTGAC
HindII 1597 GTCAAC GT[CT]^[GA]AC
2587 GTTGAC
MaeI 715 CTAG C^TAG
742 CTAG
MseI 578 TTAA T^TAA
1781 TTAA
NsiI 2173 ATGCAT ATGCA^T
2474 ATGCAT
SapI 649 GAAGAGC GCTCTTC(1/4)
254 GCTCTTC
SphI 1905 GCATGC GCATG^C
2175 GCATGC
SspBI 279 TGTACA T^GTACA
1547 TGTACA
SspI 2395 AATATT AAT^ATT
2725 AATATT
Tru9I 578 TTAA T^TAA
1781 TTAA
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AfaI AluI ApaI Asp700I
AvaII BalI BanI BanII BbvI
BcnI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI