Restriction map for
pF1KSDA0679
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BbeI BbrPI BcgI BfrI BglI
BglII BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BspHI BspMI BssHII BstBI
BstEII BstPI BstUI CfoI ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoT22I
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MluI MroI
MspA1I MvnI NarI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspI StuI SwaI
Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 2412 GTATAC GT^[AC][GT]AC
AflIII 1916 ACATGT A^C[GA][CT]GT
AocI 2164 CCTAAGG CC^T[ACGT]AGG
ApaI 879 GGGCCC GGGCC^C
AvaI 2618 CCCGGG C^[CT]CG[GA]G
AvrII 615 CCTAGG C^CTAGG
BalI 2567 TGGCCA TGG^CCA
BamHI 2390 GGATCC G^GATCC
BanI 105 GGCACC G^G[CT][GA]CC
BbvCI 2283 CCTCAGC CCTCAGC(-5/-2)
BciVI 451 GTATCC GTATCC(6/5)
BclI 858 TGATCA T^GATCA
BlnI 615 CCTAGG C^CTAGG
BsaBI 853 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 31 GAGGAG GAGGAG(10/8)
BsoBI 2618 CCCGGG C^[CT]CG[GA]G
BspLU11I 1916 ACATGT A^CATGT
BsrDI 1569 CATTGC GCAATG(2/0)
BsrFI 93 GCCGGC [GA]^CCGG[CT]
Bst1107I 2412 GTATAC GTA^TAC
BstAPI 1608 GCAGTTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2412 GTATAC GTA^TAC
Bsu36I 2164 CCTAAGG CC^T[ACGT]AGG
Cfr10I 93 GCCGGC [GA]^CCGG[CT]
DraI 1266 TTTAAA TTT^AAA
DraII 750 AGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1208 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 2164 CCTAAGG CC^T[ACGT]AGG
EcoO109I 750 AGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 2657 GAATTC G^AATTC
FbaI 858 TGATCA T^GATCA
HindIII 718 AAGCTT A^AGCTT
MamI 853 GATTATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2567 TGGCCA TGG^CCA
MscI 2567 TGGCCA TGG^CCA
NaeI 93 GCCGGC GCC^GGC
NcoI 1391 CCATGG C^CATGG
NdeI 2325 CATATG CA^TATG
NgoMIV 93 GCCGGC G^CCGGC
NheI 1143 GCTAGC G^CTAGC
NspI 1916 ACATGT [GA]CATG^[CT]
PflMI 2080 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 750 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 158 CTGCAG CTGCA^G
SmaI 2618 CCCGGG CCC^GGG
SmlI 1329 CTTGAG C^T[CT][GA]AG
SpeI 1857 ACTAGT A^CTAGT
TfiI 1522 GAATC G^A[AT]TC
Van91I 2080 CCAATTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2618 CCCGGG C^CCGGG
XmaI 2618 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Asp700I 715 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2707 GAAGGATTTC
BlpI 48 GCTCAGC GC^T[ACGT]AGC
346 GCTGAGC
Bpu1102I 48 GCTCAGC GC^T[ACGT]AGC
346 GCTGAGC
BsaI 2021 GAGACC GGTCTC(1/5)
2640 GAGACC
BsrBI 34 GAGCGG CCGCTC(-3/-3)
140 GAGCGG
BssSI 377 CACGAG CACGAG(-5/-1)
1826 CACGAG
BstXI 823 CCAGTAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1391 CCATGGACATGG
BstYI 895 AGATCC [GA]^GATC[CT]
2390 GGATCC
CelII 48 GCTCAGC GC^T[ACGT]AGC
346 GCTGAGC
DsaI 913 CCACGG C^C[GA][CT]GG
1391 CCATGG
EaeI 149 TGGCCG [CT]^GGCC[GA]
2567 TGGCCA
EcoRV 424 GATATC GAT^ATC
1936 GATATC
MflI 895 AGATCC [GA]^GATC[CT]
2390 GGATCC
SacI 46 GAGCTC GAGCT^C
818 GAGCTC
ScaI 1051 AGTACT AGT^ACT
2487 AGTACT
SexAI 476 ACCAGGT A^CC[AT]GGT
988 ACCTGGT
SfaNI 79 GCATC GCATC(5/9)
2176 GCATC
TaqI 791 TCGA T^CGA
1755 TCGA
TthHB8I 791 TCGA T^CGA
1755 TCGA
XcmI 1391 CCATGGACATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2570 CCAAAAGCATGATGG
XhoII 895 AGATCC [GA]^GATC[CT]
2390 GGATCC
XmnI 715 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2707 GAAGGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MnlI MseI
MslI MspI MunI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfcI
SspBI StyI Tru9I TseI Tsp45I
Tsp509I TspRI