Restriction map for
pF1KSDA0647
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII Acc65I AclI AcyI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AvrII BbeI BbrPI
BciVI BglII BlnI BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
Bsp1407I BspDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT22I HgaI Hin1I HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacI SacII
SbfI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SwaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 932 CGCG CG^CG
AflII 1441 CTTAAG C^TTAAG
AflIII 3211 ACATGT A^C[GA][CT]GT
Alw44I 2285 GTGCAC G^TGCAC
ApaLI 2285 GTGCAC G^TGCAC
Asp700I 3511 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 671 TGCGCA TGC^GCA
BamHI 1092 GGATCC G^GATCC
BcgI 3111 GCAGCTACGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1441 CTTAAG C^TTAAG
BlpI 339 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 339 GCTAAGC GC^T[ACGT]AGC
BsmI 1477 GCATTC GAATGC(1/-1)
BspHI 3542 TCATGA T^CATGA
BspLU11I 3211 ACATGT A^CATGT
BssSI 3527 CTCGTG CACGAG(-5/-1)
BstUI 932 CGCG CG^CG
BstXI 1823 CCAGATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 339 GCTAAGC GC^T[ACGT]AGC
DraIII 2450 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRI 3376 GAATTC G^AATTC
FseI 1198 GGCCGGCC GGCCGG^CC
FspI 671 TGCGCA TGC^GCA
HaeII 1593 GGCGCT [GA]GCGC^[CT]
HindIII 1968 AAGCTT A^AGCTT
MvnI 932 CGCG CG^CG
NaeI 1199 GCCGGC GCC^GGC
NdeI 3343 CATATG CA^TATG
NgoMIV 1199 GCCGGC G^CCGGC
NspI 3211 ACATGT [GA]CATG^[CT]
PflMI 2730 CCAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 92 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 3151 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3542 TCATGA T^CATGA
SapI 2139 GAAGAGC GCTCTTC(1/4)
ScaI 992 AGTACT AGT^ACT
SexAI 749 ACCTGGT A^CC[AT]GGT
SfiI 954 GGCCAACCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 786 CCCGGG CCC^GGG
SpeI 66 ACTAGT A^CTAGT
SspI 1266 AATATT AAT^ATT
StuI 1926 AGGCCT AGG^CCT
Van91I 2730 CCAAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2475 TCTAGA T^CTAGA
XmaCI 786 CCCGGG C^CCGGG
XmaI 786 CCCGGG C^CCGGG
XmnI 3511 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AocI 1951 CCTGAGG CC^T[ACGT]AGG
2510 CCTCAGG
AspI 196 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1651 GACATGGTC
AvaI 786 CCCGGG C^[CT]CG[GA]G
2129 CTCGGG
BglI 955 GCCAACCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1514 GCCTCTACGGC
BsgI 1687 CTGCAC GTGCAG(16/14)
508 GTGCAG
BsoBI 786 CCCGGG C^[CT]CG[GA]G
2129 CTCGGG
BspMI 360 ACCTGC ACCTGC(4/8)
1579 ACCTGC
BsrBI 630 CCGCTC CCGCTC(-3/-3)
1797 CCGCTC
BsrFI 173 ACCGGC [GA]^CCGG[CT]
1199 GCCGGC
Bsu36I 1951 CCTGAGG CC^T[ACGT]AGG
2510 CCTCAGG
Cfr10I 173 ACCGGC [GA]^CCGG[CT]
1199 GCCGGC
Eco81I 1951 CCTGAGG CC^T[ACGT]AGG
2510 CCTCAGG
FauI 692 CCCGC CCCGC(4/6)
2826 GCGGG
HincII 470 GTCGAC GT[CT]^[GA]AC
3119 GTCGAC
HindII 470 GTCGAC GT[CT]^[GA]AC
3119 GTCGAC
PflFI 196 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1651 GACATGGTC
SalI 470 GTCGAC G^TCGAC
3119 GTCGAC
SmlI 1441 CTTAAG C^T[CT][GA]AG
2058 CTTGAG
Tth111I 196 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1651 GACATGGTC
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AccIII AciI AcsI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsaWI BseAI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspEI
BsrDI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FbaI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII