################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AccII AclI AflII AflIII AgeI Aor51HI AscI AseI AsnI AviII BalI BamHI BbrPI BbvCI BcgI BciVI BfrI BglII BlpI Bpu1102I BsaAI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CelII ClaI CpoI DraI DraIII DsaI EagI EclXI Eco47III Eco52I FseI FspI HincII HindII HpaI KspI MfeI MluI MluNI MscI MunI MvnI NaeI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 818 GACGTC GACGT^C AccI 1313 GTCTAC GT^[AC][GT]AC AccIII 2780 TCCGGA T^CCGGA AhdI 1216 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1577 GTGCAC G^TGCAC AocI 1684 CCTCAGG CC^T[ACGT]AGG ApaI 2612 GGGCCC GGGCC^C ApaLI 1577 GTGCAC G^TGCAC AspEI 1216 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 3241 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2615 CCCGGG C^[CT]CG[GA]G AvrII 3325 CCTAGG C^CTAGG BbeI 2957 GGCGCC GGCGC^C BclI 2076 TGATCA T^GATCA BglI 33 GCCTGAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 3325 CCTAGG C^CTAGG BsaBI 1595 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2780 TCCGGA [AT]^CCGG[AT] BseAI 2780 TCCGGA T^CCGGA BsmBI 3943 GAGACG CGTCTC(1/5) BsoBI 2615 CCCGGG C^[CT]CG[GA]G BspEI 2780 TCCGGA T^CCGGA BspHI 1811 TCATGA T^CATGA BstEII 865 GGTTACC G^GT[ACGT]ACC BstPI 865 GGTTACC G^GT[ACGT]ACC BstXI 1794 CCAACCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1684 CCTCAGG CC^T[ACGT]AGG DrdI 764 GACCTCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1450 CGGCCA [CT]^GGCC[GA] Eam1105I 1216 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1684 CCTCAGG CC^T[ACGT]AGG EcoNI 210 CCTCCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 865 GGTTACC G^GT[ACGT]ACC EcoRV 1807 GATATC GAT^ATC FbaI 2076 TGATCA T^GATCA KasI 2957 GGCGCC G^GCGCC MamI 1595 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2780 TCCGGA T^CCGGA MslI 1812 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 2957 GGCGCC GG^CGCC NdeI 1864 CATATG CA^TATG PflFI 3241 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2119 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1811 TCATGA T^CATGA SexAI 1200 ACCAGGT A^CC[AT]GGT SfoI 2957 GGCGCC GGC^GCC SmaI 2615 CCCGGG CCC^GGG SmlI 3637 CTTGAG C^T[CT][GA]AG Tsp45I 494 GTGAC ^GT[GC]AC Tth111I 3241 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2119 CCAGATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2888 CCACGCTGTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 2615 CCCGGG C^CCGGG XmaI 2615 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### Acc65I 243 GGTACC G^GTACC 3052 GGTACC AcyI 818 GACGTC G[GA]^CG[CT]C 2957 GGCGCC Asp718I 243 GGTACC G^GTACC 3052 GGTACC BcnI 2615 CCCGG CC^[GC]GG 2616 CCGGG BsaHI 818 GACGTC G[GA]^CG[CT]C 2957 GGCGCC BsaI 600 GAGACC GGTCTC(1/5) 1522 GGTCTC BsgI 3172 CTGCAC GTGCAG(16/14) 3835 GTGCAG BsmI 979 GCATTC GAATGC(1/-1) 3162 GCATTC BspMI 1934 ACCTGC ACCTGC(4/8) 3170 ACCTGC BsrDI 95 CATTGC GCAATG(2/0) 1657 GCAATG BsrFI 2654 ACCGGC [GA]^CCGG[CT] 3389 ACCGGC BstAPI 1929 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3681 GCAGCAGCTGC Cfr10I 2654 ACCGGC [GA]^CCGG[CT] 3389 ACCGGC EcoRI 3189 GAATTC G^AATTC 3330 GAATTC HaeII 2957 GGCGCC [GA]GCGC^[CT] 3641 AGCGCC Hin1I 818 GACGTC G[GA]^CG[CT]C 2957 GGCGCC HindIII 1788 AAGCTT A^AGCTT 2827 AAGCTT KpnI 243 GGTACC GGTAC^C 3052 GGTACC NciI 2615 CCCGG CC^[GC]GG 2616 CCGGG NspI 554 GCATGT [GA]CATG^[CT] 2872 GCATGT PpuMI 1701 AGGTCCC [GA]G^G[AT]CC[CT] 3321 GGGACCT PstI 791 CTGCAG CTGCA^G 1936 CTGCAG PvuII 2812 CAGCTG CAG^CTG 3685 CAGCTG SacI 47 GAGCTC GAGCT^C 3146 GAGCTC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NsiI PleI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII XmnI