Restriction map for
pF1KSDA0637
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
AocI Aor51HI ApaI AscI AseI
AsnI AspI AviII AvrII BciVI
BlnI BsiWI Bsp1407I BspDI BsrDI
BsrGI Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraIII DrdI Eco47III
Eco81I EcoRV FseI FspI HincII
HindII HindIII HpaI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PmeI PshBI RsrII SalI SbfI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I XbaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 2659 GACGTC GACGT^C
Acc65I 1786 GGTACC G^GTACC
AccI 2026 GTAGAC GT^[AC][GT]AC
AccIII 1872 TCCGGA T^CCGGA
AclI 2032 AACGTT AA^CGTT
AflII 2158 CTTAAG C^TTAAG
AgeI 2582 ACCGGT A^CCGGT
Alw44I 2578 GTGCAC G^TGCAC
AlwNI 2539 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2578 GTGCAC G^TGCAC
Asp718I 1786 GGTACC G^GTACC
BamHI 3024 GGATCC G^GATCC
BbeI 1508 GGCGCC GGCGC^C
BbrPI 478 CACGTG CAC^GTG
BcgI 789 CGATGAACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2762 TGATCA T^GATCA
BfaI 1313 CTAG C^TAG
BfrI 2158 CTTAAG C^TTAAG
BglII 2564 AGATCT A^GATCT
BsaAI 478 CACGTG [CT]AC^GT[GA]
BsaBI 2925 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1845 GGTCTC GGTCTC(1/5)
BseAI 1872 TCCGGA T^CCGGA
BspEI 1872 TCCGGA T^CCGGA
BssSI 969 CACGAG CACGAG(-5/-1)
BstXI 3014 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 959 AGGACCT [GA]G^G[ACGT]CC[CT]
EagI 2272 CGGCCG C^GGCCG
EclXI 2272 CGGCCG C^GGCCG
Eco52I 2272 CGGCCG C^GGCCG
EcoO109I 959 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 405 ATGCAT ATGCA^T
FbaI 2762 TGATCA T^GATCA
KasI 1508 GGCGCC G^GCGCC
KpnI 1786 GGTACC GGTAC^C
MaeI 1313 CTAG C^TAG
MamI 2925 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1872 TCCGGA T^CCGGA
NarI 1508 GGCGCC GG^CGCC
NsiI 405 ATGCAT ATGCA^T
PinAI 2582 ACCGGT A^CCGGT
PmaCI 478 CACGTG CAC^GTG
PmlI 478 CACGTG CAC^GTG
PpuMI 959 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1367 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2032 AACGTT AA^CGTT
PvuI 657 CGATCG CGAT^CG
SfiI 291 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1508 GGCGCC GGC^GCC
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcsI 92 GAATTC [GA]^AATT[CT]
3108 GAATTC
AhdI 1186 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2659 GACGTCCCGTC
ApoI 92 GAATTC [GA]^AATT[CT]
3108 GAATTC
AspEI 1186 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2659 GACGTCCCGTC
BanII 336 GAGCTC G[GA]GC[CT]^C
1102 GAGCTC
BlpI 1104 GCTCAGC GC^T[ACGT]AGC
2547 GCTGAGC
Bpu1102I 1104 GCTCAGC GC^T[ACGT]AGC
2547 GCTGAGC
BsgI 2473 GTGCAG GTGCAG(16/14)
2536 GTGCAG
BsmI 917 GCATTC GAATGC(1/-1)
2348 GCATTC
BspLU11I 713 ACATGT A^CATGT
989 ACATGT
BssHII 251 GCGCGC G^CGCGC
486 GCGCGC
BstEII 589 GGTGACC G^GT[ACGT]ACC
2385 GGTGACC
BstPI 589 GGTGACC G^GT[ACGT]ACC
2385 GGTGACC
CelII 1104 GCTCAGC GC^T[ACGT]AGC
2547 GCTGAGC
DraI 64 TTTAAA TTT^AAA
124 TTTAAA
Eam1105I 1186 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2659 GACGTCCCGTC
EcoO65I 589 GGTGACC G^GT[ACGT]ACC
2385 GGTGACC
EcoRI 92 GAATTC G^AATTC
3108 GAATTC
KspI 1888 CCGCGG CCGC^GG
2998 CCGCGG
PaeR7I 2466 CTCGAG C^TCGAG
2695 CTCGAG
PflMI 627 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2526 CCAGCGGATGG
SacI 336 GAGCTC GAGCT^C
1102 GAGCTC
SacII 1888 CCGCGG CCGC^GG
2998 CCGCGG
SapI 816 GAAGAGC GCTCTTC(1/4)
2910 GCTCTTC
ScaI 218 AGTACT AGT^ACT
2072 AGTACT
SmaI 210 CCCGGG CCC^GGG
1880 CCCGGG
StuI 1265 AGGCCT AGG^CCT
3038 AGGCCT
Van91I 627 CCAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2526 CCAGCGGATGG
XcmI 2184 CCACTTCACGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3440 CCAGGCTCATGATGG
XhoI 2466 CTCGAG C^TCGAG
2695 CTCGAG
XmaCI 210 CCCGGG C^CCGGG
1880 CCCGGG
XmaI 210 CCCGGG C^CCGGG
1880 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI Asp700I AspHI AvaI
AvaII BalI BanI BbsI BbvCI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI