Restriction map for
pF1KSDA0372
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# NO CUT ENZYMES ( 102 enzymes) #
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AatII AccII AccIII AclI AgeI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspI AviII BamHI BbeI
BbrPI BcgI BcnI BglII BsaAI
BsaWI BseAI BsiEI BspDI BspEI
BspMI BsrBI BsrFI BssHII Bst1107I
BstBI BstUI BstXI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoRV FauI
FseI FspI HaeII HapII HhaI
HinP1I HpaI HpaII KasI KspI
MluI MroI MspI MvnI NaeI
NarI NciI NdeI NgoMIV NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 4571 GGTACC G^GTACC
AcyI 4505 GGCGTC G[GA]^CG[CT]C
AflIII 4221 ACATGT A^C[GA][CT]GT
AhdI 4500 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 4571 GGTACC G^GTACC
AspEI 4500 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3599 CTCGAG C^[CT]CG[GA]G
AvrII 1932 CCTAGG C^CTAGG
BalI 4387 TGGCCA TGG^CCA
BbvCI 2713 GCTGAGG CCTCAGC(-5/-2)
BlnI 1932 CCTAGG C^CTAGG
BlpI 2536 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2536 GCTCAGC GC^T[ACGT]AGC
BsaBI 687 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4505 GGCGTC G[GA]^CG[CT]C
BsiWI 1120 CGTACG C^GTACG
BsmBI 1511 GAGACG CGTCTC(1/5)
BsmFI 3966 GTCCC GGGAC(10/14)
BsoBI 3599 CTCGAG C^[CT]CG[GA]G
BspLU11I 4221 ACATGT A^CATGT
BssSI 1538 CTCGTG CACGAG(-5/-1)
BstEII 3571 GGTGACC G^GT[ACGT]ACC
BstPI 3571 GGTGACC G^GT[ACGT]ACC
CelII 2536 GCTCAGC GC^T[ACGT]AGC
DsaI 2738 CCATGG C^C[GA][CT]GG
EaeI 4387 TGGCCA [CT]^GGCC[GA]
Eam1105I 4500 GACACGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1697 CCTGGCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3571 GGTGACC G^GT[ACGT]ACC
EcoRI 2924 GAATTC G^AATTC
Hin1I 4505 GGCGTC G[GA]^CG[CT]C
KpnI 4571 GGTACC GGTAC^C
MamI 687 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4387 TGGCCA TGG^CCA
MscI 4387 TGGCCA TGG^CCA
MspA1I 149 CAGCTG C[AC]G^C[GT]G
NcoI 2738 CCATGG C^CATGG
NheI 2154 GCTAGC G^CTAGC
PaeR7I 3599 CTCGAG C^TCGAG
PvuII 149 CAGCTG CAG^CTG
SapI 460 GAAGAGC GCTCTTC(1/4)
ScaI 606 AGTACT AGT^ACT
SspI 2102 AATATT AAT^ATT
SwaI 3333 ATTTAAAT ATTT^AAAT
XcmI 4201 CCAGCTTGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3599 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AciI 3519 CCGC CCGC(-3/-1)
3709 GCGG
AflII 1824 CTTAAG C^TTAAG
2407 CTTAAG
AseI 2350 ATTAAT AT^TAAT
2575 ATTAAT
AsnI 2350 ATTAAT AT^TAAT
2575 ATTAAT
BanI 3482 GGTGCC G^G[CT][GA]CC
4571 GGTACC
BfrI 1824 CTTAAG C^TTAAG
2407 CTTAAG
BglI 821 GCCTCATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1270 GCCCTTGAGGC
BsaI 3587 GGTCTC GGTCTC(1/5)
3980 GGTCTC
BseRI 2272 CTCCTC GAGGAG(10/8)
1832 GAGGAG
Bsp1407I 705 TGTACA T^GTACA
3701 TGTACA
BsrGI 705 TGTACA T^GTACA
3701 TGTACA
HgaI 1021 GCGTC GACGC(5/10)
4506 GCGTC
NspI 2503 GCATGT [GA]CATG^[CT]
4221 ACATGT
PshBI 2350 ATTAAT AT^TAAT
2575 ATTAAT
SspBI 705 TGTACA T^GTACA
3701 TGTACA
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# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BciVI BclI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmI Bsp1286I BspHI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I EcoT22I FbaI
Fnu4HI FokI HaeIII HincII HindII
HindIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MunI MvaI MwoI NdeII NlaIII
NlaIV NsiI PleI PstI RcaI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI