Restriction map for
pF1KSDA0194
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AclI AflII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
BbeI BbrPI BciVI BfrI BglII
BsaAI BsaBI BsiEI BsiWI BspDI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstZ17I Cfr10I ClaI
CpoI DraI DrdI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI ScaI SexAI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI SspI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 1894 GTCTAC GT^[AC][GT]AC
AccIII 345 TCCGGA T^CCGGA
AhdI 1656 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1348 CCTGAGG CC^T[ACGT]AGG
ApaI 2296 GGGCCC GGGCC^C
AspEI 1656 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1291 CCCGGG C^[CT]CG[GA]G
AviII 2078 TGCGCA TGC^GCA
AvrII 1017 CCTAGG C^CTAGG
BalI 3305 TGGCCA TGG^CCA
BcgI 1908 GCACAAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1253 TGATCA T^GATCA
BlnI 1017 CCTAGG C^CTAGG
BlpI 2568 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2568 GCTGAGC GC^T[ACGT]AGC
BseAI 345 TCCGGA T^CCGGA
BsmBI 1080 CGTCTC CGTCTC(1/5)
BsmI 2013 GAATGC GAATGC(1/-1)
BsoBI 1291 CCCGGG C^[CT]CG[GA]G
BspEI 345 TCCGGA T^CCGGA
BstEII 3544 GGTGACC G^GT[ACGT]ACC
BstPI 3544 GGTGACC G^GT[ACGT]ACC
BstXI 3308 CCACGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1348 CCTGAGG CC^T[ACGT]AGG
CelII 2568 GCTGAGC GC^T[ACGT]AGC
DraIII 2238 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 933 CCATGG C^C[GA][CT]GG
Eam1105I 1656 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1348 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3544 GGTGACC G^GT[ACGT]ACC
FbaI 1253 TGATCA T^GATCA
FspI 2078 TGCGCA TGC^GCA
HaeII 2054 AGCGCC [GA]GCGC^[CT]
HindIII 2995 AAGCTT A^AGCTT
MluNI 3305 TGGCCA TGG^CCA
MroI 345 TCCGGA T^CCGGA
MscI 3305 TGGCCA TGG^CCA
NcoI 933 CCATGG C^CATGG
NheI 2709 GCTAGC G^CTAGC
NspI 940 ACATGC [GA]CATG^[CT]
PshAI 1320 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 2718 GAAGAGC GCTCTTC(1/4)
SbfI 411 CCTGCAGG CCTGCA^GG
SfiI 1611 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1291 CCCGGG CCC^GGG
Sse8387I 411 CCTGCAGG CCTGCA^GG
XmaCI 1291 CCCGGG C^CCGGG
XmaI 1291 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 3506 CGCG CG^CG
3721 CGCG
AcyI 1078 GGCGTC G[GA]^CG[CT]C
1320 GACGCC
BamHI 306 GGATCC G^GATCC
1031 GGATCC
BsaHI 1078 GGCGTC G[GA]^CG[CT]C
1320 GACGCC
BsaI 712 GAGACC GGTCTC(1/5)
2413 GGTCTC
BsrDI 3657 CATTGC GCAATG(2/0)
2594 GCAATG
BstUI 3506 CGCG CG^CG
3721 CGCG
EcoRI 1176 GAATTC G^AATTC
2743 GAATTC
FauI 923 CCCGC CCCGC(4/6)
3272 CCCGC
Hin1I 1078 GGCGTC G[GA]^CG[CT]C
1320 GACGCC
HpaI 1573 GTTAAC GTT^AAC
1939 GTTAAC
MslI 989 CACCTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3309 CACGTCAGTG
MvnI 3506 CGCG CG^CG
3721 CGCG
NdeI 11 CATATG CA^TATG
968 CATATG
PpuMI 477 AGGACCT [GA]G^G[AT]CC[CT]
2492 AGGACCC
StuI 1619 AGGCCT AGG^CCT
3525 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BspMI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII