Restriction map for
pF1KB9409
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AvrII BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrFI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI DraI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FbaI FseI Hin1I
HincII HindII HpaI KasI KpnI
MaeI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TfiI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AocI 397 CCTCAGG CC^T[ACGT]AGG
Aor51HI 104 AGCGCT AGC^GCT
BamHI 802 GGATCC G^GATCC
BbsI 226 GTCTTC GAAGAC(2/6)
BcnI 27 CCCGG CC^[GC]GG
BpuAI 226 GTCTTC GAAGAC(2/6)
BsaI 340 GAGACC GGTCTC(1/5)
BsgI 139 GTGCAG GTGCAG(16/14)
BsiEI 69 CGACCG CG[GA][CT]^CG
BsmBI 65 GAGACG CGTCTC(1/5)
BspHI 468 TCATGA T^CATGA
BsrDI 552 CATTGC GCAATG(2/0)
BssSI 16 CACGAG CACGAG(-5/-1)
BstYI 802 GGATCC [GA]^GATC[CT]
Bsu36I 397 CCTCAGG CC^T[ACGT]AGG
CpoI 29 CGGTCCG CG^G[AT]CCG
DraII 269 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 16 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 356 CCGCGG C^C[GA][CT]GG
EarI 220 GAAGAG CTCTTC(1/4)
Eco47III 104 AGCGCT AGC^GCT
Eco81I 397 CCTCAGG CC^T[ACGT]AGG
EcoO109I 269 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRV 856 GATATC GAT^ATC
HaeII 104 AGCGCT [GA]GCGC^[CT]
HapII 28 CCGG C^CGG
HgaI 74 GCGTC GACGC(5/10)
HindIII 731 AAGCTT A^AGCTT
HinfI 276 GACTC G^A[ACGT]TC
HpaII 28 CCGG C^CGG
Ksp632I 220 GAAGAG CTCTTC(1/4)
KspI 356 CCGCGG CCGC^GG
MflI 802 GGATCC [GA]^GATC[CT]
MseI 575 TTAA T^TAA
MspI 28 CCGG C^CGG
NciI 27 CCCGG CC^[GC]GG
PflMI 15 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 276 GACTC GAGTC(4/5)
PpuMI 269 GGGTCCC [GA]G^G[AT]CC[CT]
PvuII 455 CAGCTG CAG^CTG
RcaI 468 TCATGA T^CATGA
RsrII 29 CGGTCCG CG^G[AT]CCG
SacI 660 GAGCTC GAGCT^C
SacII 356 CCGCGG CCGC^GG
SphI 537 GCATGC GCATG^C
Tru9I 575 TTAA T^TAA
Tsp509I 244 AATT ^AATT
TspRI 118 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 15 CCACGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 802 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 73 CGCG CG^CG
357 CGCG
AfaI 588 GTAC GT^AC
780 GTAC
AlwNI 82 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
400 CAGGAGCTG
AspI 25 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
70 GACCGCGTC
AviII 164 TGCGCA TGC^GCA
491 TGCGCA
BalI 176 TGGCCA TGG^CCA
236 TGGCCA
BanII 637 GGGCTC G[GA]GC[CT]^C
660 GAGCTC
BsaJI 300 CCTGGG C^C[ACGT][ACGT]GG
356 CCGCGG
BspMI 56 ACCTGC ACCTGC(4/8)
86 ACCTGC
BstAPI 81 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
483 GCACATCTTGC
BstUI 73 CGCG CG^CG
357 CGCG
DrdI 67 GACGACCGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
276 GACTCCGAAGTC
EaeI 176 TGGCCA [CT]^GGCC[GA]
236 TGGCCA
FspI 164 TGCGCA TGC^GCA
491 TGCGCA
HphI 92 TCACC GGTGA(8/7)
704 TCACC
MaeIII 460 GTCAC ^GT[ACGT]AC
797 GTGAC
MluNI 176 TGGCCA TGG^CCA
236 TGGCCA
MscI 176 TGGCCA TGG^CCA
236 TGGCCA
MslI 180 CACCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
711 CACAGACGTG
MvnI 73 CGCG CG^CG
357 CGCG
PflFI 25 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
70 GACCGCGTC
PstI 79 CTGCAG CTGCA^G
772 CTGCAG
RsaI 588 GTAC GT^AC
780 GTAC
SfaNI 186 GATGC GCATC(5/9)
676 GATGC
Tsp45I 460 GTCAC ^GT[GC]AC
797 GTGAC
Tth111I 25 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
70 GACCGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AlwI AspHI AvaII
BbvI BmyI BpmI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
FauI Fnu4HI FokI HaeIII HhaI
HinP1I ItaI MaeII MboI MboII
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV NspI Sau3AI Sau96I
ScrFI SfcI TaqI TseI TthHB8I
XcmI