Restriction map for
pF1KB9390
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BclI BcnI BfaI
BfrI BglI BglII BlnI BsaAI
BsaBI BsiWI BsmBI BsmFI BsmI
Bsp1407I BspDI BspHI BspLU11I BspMI
BsrFI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI HaeII HincII HindII HindIII
HpaI HphI KasI KpnI KspI
MaeI MamI MfeI MluI MluNI
MscI MseI MslI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI TfiI Tru9I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccIII 41 TCCGGA T^CCGGA
AcsI 317 AAATTC [GA]^AATT[CT]
AcyI 64 GACGCC G[GA]^CG[CT]C
AfaI 531 GTAC GT^AC
AlwNI 223 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 317 AAATTC [GA]^AATT[CT]
AspHI 442 GAGCTC G[AT]GC[AT]^C
AvaI 602 CTCGGG C^[CT]CG[GA]G
AviII 29 TGCGCA TGC^GCA
BanI 85 GGCACC G^G[CT][GA]CC
BbsI 544 GAAGAC GAAGAC(2/6)
BlpI 439 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 439 GCTGAGC GC^T[ACGT]AGC
BpuAI 544 GAAGAC GAAGAC(2/6)
BsaHI 64 GACGCC G[GA]^CG[CT]C
BsaI 127 GAGACC GGTCTC(1/5)
BsaJI 197 CCCAGG C^C[ACGT][ACGT]GG
BsaWI 41 TCCGGA [AT]^CCGG[AT]
BseAI 41 TCCGGA T^CCGGA
BsgI 425 GTGCAG GTGCAG(16/14)
BsiEI 366 CGACCG CG[GA][CT]^CG
BsiHKAI 442 GAGCTC G[AT]GC[AT]^C
BsoBI 602 CTCGGG C^[CT]CG[GA]G
BspEI 41 TCCGGA T^CCGGA
BsrBI 433 GAGCGG CCGCTC(-3/-3)
BsrDI 351 CATTGC GCAATG(2/0)
BsrI 510 ACTGG ACTGG(1/-1)
BssHII 78 GCGCGC G^CGCGC
BssSI 109 CACGAG CACGAG(-5/-1)
BstF5I 165 CATCC GGATG(2/0)
CelII 439 GCTGAGC GC^T[ACGT]AGC
DraII 569 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoNI 723 CCTGAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 569 AGGTCCT [GA]G^G[ACGT]CC[CT]
FokI 165 CATCC GGATG(9/13)
FspI 29 TGCGCA TGC^GCA
HapII 42 CCGG C^CGG
Hin1I 64 GACGCC G[GA]^CG[CT]C
HpaII 42 CCGG C^CGG
MroI 41 TCCGGA T^CCGGA
MspI 42 CCGG C^CGG
NlaIII 270 CATG CATG^
PpuMI 569 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 169 CAGCTG CAG^CTG
RsaI 531 GTAC GT^AC
SacI 442 GAGCTC GAGCT^C
SbfI 48 CCTGCAGG CCTGCA^GG
ScaI 530 AGTACT AGT^ACT
SmlI 682 CTTGAG C^T[CT][GA]AG
Sse8387I 48 CCTGCAGG CCTGCA^GG
TaqI 288 TCGA T^CGA
TspRI 509 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 288 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaII 105 GGACC G^G[AT]CC
570 GGTCC
BanII 290 GAGCCC G[GA]GC[CT]^C
442 GAGCTC
BbvCI 70 GCTGAGG CCTCAGC(-5/-2)
120 GCTGAGG
BbvI 93 GCAGC GCAGC(8/12)
247 GCAGC
BseRI 73 GAGGAG GAGGAG(10/8)
430 GAGGAG
BsiYI 390 CCGTAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
723 CCTGAATGAGG
BslI 390 CCGTAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
723 CCTGAATGAGG
BsmAI 127 GAGAC GTCTC(1/5)
598 GAGAC
BssKI 198 CCAGG ^CC[ACGT]GG
417 CCTGG
BstNI 198 CCAGG CC^[AT]GG
417 CCTGG
BstYI 38 AGATCC [GA]^GATC[CT]
326 AGATCC
EcoRII 198 CCAGG ^CC[AT]GG
417 CCTGG
HaeIII 387 GGCC GG^CC
720 GGCC
HinfI 285 GAGTC G^A[ACGT]TC
600 GACTC
MaeII 143 ACGT A^CGT
423 ACGT
MflI 38 AGATCC [GA]^GATC[CT]
326 AGATCC
MvaI 198 CCAGG CC^[AT]GG
417 CCTGG
NlaIV 44 GGAGCC GG[ACGT]^[ACGT]CC
85 GGCACC
PleI 600 GACTC GAGTC(4/5)
285 GAGTC
PstI 49 CTGCAG CTGCA^G
91 CTGCAG
ScrFI 198 CCAGG CC^[ACGT]GG
417 CCTGG
SfaNI 361 GATGC GCATC(5/9)
164 GCATC
SfcI 49 CTGCAG C^T[GA][CT]AG
91 CTGCAG
TseI 93 GCAGC G^C[AT]GC
247 GCAGC
Tsp45I 490 GTGAC ^GT[GC]AC
507 GTCAC
XhoII 38 AGATCC [GA]^GATC[CT]
326 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AccII AciI AluI AlwI BmyI
BpmI Bsp1286I BstUI Cac8I CfoI
Cfr13I DdeI DpnII EarI FauI
Fnu4HI HgaI HhaI HinP1I ItaI
Ksp632I MaeIII MboI MboII MnlI
MspA1I MvnI MwoI NdeII Sau3AI
Sau96I Tsp509I