Restriction map for
pF1KB7023
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# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
Alw44I AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AvrII BbeI BciVI BfrI BlnI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco52I Eco81I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KasI
KpnI MamI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 2278 GACGTC GACGT^C
AccI 616 GTCTAC GT^[AC][GT]AC
AccIII 2613 TCCGGA T^CCGGA
AcyI 2278 GACGTC G[GA]^CG[CT]C
Aor51HI 1081 AGCGCT AGC^GCT
AviII 1529 TGCGCA TGC^GCA
BamHI 2669 GGATCC G^GATCC
BbsI 850 GAAGAC GAAGAC(2/6)
BbvCI 1207 GCTGAGG CCTCAGC(-5/-2)
BpuAI 850 GAAGAC GAAGAC(2/6)
BsaHI 2278 GACGTC G[GA]^CG[CT]C
BseAI 2613 TCCGGA T^CCGGA
BspEI 2613 TCCGGA T^CCGGA
BspHI 1961 TCATGA T^CATGA
BsrFI 777 GCCGGT [GA]^CCGG[CT]
BstEII 2758 GGTTACC G^GT[ACGT]ACC
BstPI 2758 GGTTACC G^GT[ACGT]ACC
Cfr10I 777 GCCGGT [GA]^CCGG[CT]
Eco47III 1081 AGCGCT AGC^GCT
EcoNI 954 CCTGGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2758 GGTTACC G^GT[ACGT]ACC
FspI 1529 TGCGCA TGC^GCA
Hin1I 2278 GACGTC G[GA]^CG[CT]C
KspI 1786 CCGCGG CCGC^GG
MroI 2613 TCCGGA T^CCGGA
NcoI 2985 CCATGG C^CATGG
NspI 1319 GCATGC [GA]CATG^[CT]
PflMI 2910 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 666 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1961 TCATGA T^CATGA
SacII 1786 CCGCGG CCGC^GG
ScaI 2045 AGTACT AGT^ACT
SmaI 111 CCCGGG CCC^GGG
SphI 1319 GCATGC GCATG^C
StuI 1022 AGGCCT AGG^CCT
Van91I 2910 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 111 CCCGGG C^CCGGG
XmaI 111 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 1112 ACGTGT A^C[GA][CT]GT
2654 ACGTGT
AhdI 2293 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2653 GACGTGTGGTC
AlwNI 91 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1166 CAGAGGCTG
ApaI 1975 GGGCCC GGGCC^C
3105 GGGCCC
AspEI 2293 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2653 GACGTGTGGTC
BcgI 704 GCATTGTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1494 GCACGTGGATCG
BclI 519 TGATCA T^GATCA
2372 TGATCA
BglI 1609 GCCATCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2160 GCCCGTTGGGC
BlpI 982 GCTGAGC GC^T[ACGT]AGC
2391 GCTAAGC
Bpu1102I 982 GCTGAGC GC^T[ACGT]AGC
2391 GCTAAGC
BsaWI 174 TCCGGT [AT]^CCGG[AT]
2613 TCCGGA
BsmBI 645 CGTCTC CGTCTC(1/5)
2276 GAGACG
BsrDI 872 GCAATG GCAATG(2/0)
2150 GCAATG
BssSI 481 CACGAG CACGAG(-5/-1)
2407 CTCGTG
CelII 982 GCTGAGC GC^T[ACGT]AGC
2391 GCTAAGC
Eam1105I 2293 GACATCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2653 GACGTGTGGTC
EarI 1411 GAAGAG CTCTTC(1/4)
2824 GAAGAG
FbaI 519 TGATCA T^GATCA
2372 TGATCA
HaeII 1081 AGCGCT [GA]GCGC^[CT]
2120 AGCGCC
Ksp632I 1411 GAAGAG CTCTTC(1/4)
2824 GAAGAG
MseI 1589 TTAA T^TAA
1907 TTAA
PaeR7I 2543 CTCGAG C^TCGAG
2872 CTCGAG
SexAI 2063 ACCTGGT A^CC[AT]GGT
2423 ACCAGGT
Tru9I 1589 TTAA T^TAA
1907 TTAA
Tsp509I 977 AATT ^AATT
2566 AATT
XhoI 2543 CTCGAG C^TCGAG
2872 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbvI BcnI BfaI
BglII BmyI BpmI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PmaCI
PmlI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I TspRI TthHB8I XcmI
XhoII