Restriction map for
pF1KB7013
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI AseI
AsnI Asp700I AspEI AviII BamHI
BbeI BbrPI BfrI BglII BsaBI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FseI
FspI HpaI KasI KspI MamI
MluI MroI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2050 GGTACC G^GTACC
AccII 3222 CGCG CG^CG
AcyI 2248 GGCGTC G[GA]^CG[CT]C
Alw44I 2585 GTGCAC G^TGCAC
AlwI 3055 GATCC GGATC(4/5)
ApaLI 2585 GTGCAC G^TGCAC
Asp718I 2050 GGTACC G^GTACC
AspHI 2585 GTGCAC G[AT]GC[AT]^C
AspI 1413 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1878 CTCGGG C^[CT]CG[GA]G
AvrII 1872 CCTAGG C^CTAGG
BcgI 733 GCAGCAAAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 309 TGATCA T^GATCA
BlnI 1872 CCTAGG C^CTAGG
BsaAI 77 TACGTG [CT]AC^GT[GA]
BsaHI 2248 GGCGTC G[GA]^CG[CT]C
BsiHKAI 2585 GTGCAC G[AT]GC[AT]^C
BsoBI 1878 CTCGGG C^[CT]CG[GA]G
BspHI 417 TCATGA T^CATGA
BsrFI 1804 GCCGGC [GA]^CCGG[CT]
BssSI 36 CTCGTG CACGAG(-5/-1)
BstEII 1503 GGTGACC G^GT[ACGT]ACC
BstPI 1503 GGTGACC G^GT[ACGT]ACC
BstUI 3222 CGCG CG^CG
BstYI 3054 AGATCC [GA]^GATC[CT]
Cfr10I 1804 GCCGGC [GA]^CCGG[CT]
DraI 432 TTTAAA TTT^AAA
DrdI 2488 GACATGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 3123 GAAGAG CTCTTC(1/4)
EcoO65I 1503 GGTGACC G^GT[ACGT]ACC
FbaI 309 TGATCA T^GATCA
HaeII 135 AGCGCC [GA]GCGC^[CT]
Hin1I 2248 GGCGTC G[GA]^CG[CT]C
KpnI 2050 GGTACC GGTAC^C
Ksp632I 3123 GAAGAG CTCTTC(1/4)
MflI 3054 AGATCC [GA]^GATC[CT]
MvnI 3222 CGCG CG^CG
NaeI 1804 GCCGGC GCC^GGC
NdeI 2948 CATATG CA^TATG
NgoMIV 1804 GCCGGC G^CCGGC
PflFI 1413 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2791 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 61 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 417 TCATGA T^CATGA
SexAI 533 ACCAGGT A^CC[AT]GGT
SfiI 1140 GGCCACTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 3077 GCATGC GCATG^C
SspI 1327 AATATT AAT^ATT
Tth111I 1413 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2791 CCAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 3054 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 74 GTCTAC GT^[AC][GT]AC
931 GTAGAC
ApaI 2004 GGGCCC GGGCC^C
2531 GGGCCC
BalI 584 TGGCCA TGG^CCA
1139 TGGCCA
BbsI 1356 GAAGAC GAAGAC(2/6)
1410 GAAGAC
BbvCI 1042 GCTGAGG CCTCAGC(-5/-2)
2932 GCTGAGG
BciVI 1454 GGATAC GTATCC(6/5)
1650 GTATCC
BcnI 1200 CCCGG CC^[GC]GG
2002 CCGGG
BglI 1141 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2322 GCCTGGAAGGC
BpuAI 1356 GAAGAC GAAGAC(2/6)
1410 GAAGAC
BsgI 2540 GTGCAG GTGCAG(16/14)
2807 GTGCAG
BspLU11I 1748 ACATGT A^CATGT
3214 ACATGT
BstXI 1130 CCAGCCGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2213 CCAAGATTGTGG
DsaI 581 CCATGG C^C[GA][CT]GG
2705 CCATGG
EcoRI 152 GAATTC G^AATTC
2882 GAATTC
HincII 1419 GTCAAC GT[CT]^[GA]AC
1705 GTCAAC
HindII 1419 GTCAAC GT[CT]^[GA]AC
1705 GTCAAC
HindIII 290 AAGCTT A^AGCTT
2397 AAGCTT
MluNI 584 TGGCCA TGG^CCA
1139 TGGCCA
MscI 584 TGGCCA TGG^CCA
1139 TGGCCA
NciI 1200 CCCGG CC^[GC]GG
2002 CCGGG
NcoI 581 CCATGG C^CATGG
2705 CCATGG
XcmI 1508 CCACTTTTGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2828 CCATTGCTGATTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI AvaII BanI BanII
BbvI BfaI BlpI BmyI BpmI
Bpu1102I BsaI BsaJI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MfeI MnlI
MseI MslI MspA1I MspI MunI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I