Restriction map for
pF1KB7004
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# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI AspEI AviII BbeI
BbrPI BciVI BclI BfrI BsaHI
BsgI BsiEI BsiWI Bsp1407I BspDI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI EagI
Eam1105I EclXI Eco52I EcoO65I FbaI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KspI MluI NarI
NheI NotI NruI NspV PacI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
StuI SwaI Van91I
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 3129 GGTACC G^GTACC
AccII 2293 CGCG CG^CG
AccIII 3639 TCCGGA T^CCGGA
AflIII 311 ACGTGT A^C[GA][CT]GT
AocI 2008 CCTGAGG CC^T[ACGT]AGG
Aor51HI 857 AGCGCT AGC^GCT
Asp718I 3129 GGTACC G^GTACC
AspI 1529 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1023 CCTAGG C^CTAGG
BbvCI 2970 CCTCAGC CCTCAGC(-5/-2)
BcgI 1290 GCACGGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 3396 AGATCT A^GATCT
BlnI 1023 CCTAGG C^CTAGG
BlpI 2056 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2056 GCTCAGC GC^T[ACGT]AGC
BsaAI 310 TACGTG [CT]AC^GT[GA]
BsaBI 1933 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3639 TCCGGA T^CCGGA
BsmBI 378 CGTCTC CGTCTC(1/5)
BspEI 3639 TCCGGA T^CCGGA
BspHI 3923 TCATGA T^CATGA
BsrDI 2827 GCAATG GCAATG(2/0)
BsrFI 2213 GCCGGC [GA]^CCGG[CT]
BstUI 2293 CGCG CG^CG
Bsu36I 2008 CCTGAGG CC^T[ACGT]AGG
CelII 2056 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2213 GCCGGC [GA]^CCGG[CT]
DraIII 926 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1273 GACATGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 857 AGCGCT AGC^GCT
Eco81I 2008 CCTGAGG CC^T[ACGT]AGG
EcoT22I 1 ATGCAT ATGCA^T
HincII 1948 GTCAAC GT[CT]^[GA]AC
HindII 1948 GTCAAC GT[CT]^[GA]AC
KpnI 3129 GGTACC GGTAC^C
MamI 1933 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2186 CAATTG C^AATTG
MroI 3639 TCCGGA T^CCGGA
MunI 2186 CAATTG C^AATTG
MvnI 2293 CGCG CG^CG
NaeI 2213 GCCGGC GCC^GGC
NgoMIV 2213 GCCGGC G^CCGGC
NsiI 1 ATGCAT ATGCA^T
PaeR7I 3500 CTCGAG C^TCGAG
PflFI 1529 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 720 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3923 TCATGA T^CATGA
SapI 746 GCTCTTC GCTCTTC(1/4)
SexAI 1530 ACCAGGT A^CC[AT]GGT
SspI 3491 AATATT AAT^ATT
Tth111I 1529 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1525 TCTAGA T^CTAGA
XhoI 3500 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
Asp700I 909 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1108 GAAATGGTTC
BamHI 1957 GGATCC G^GATCC
2612 GGATCC
BsaI 3690 GAGACC GGTCTC(1/5)
1702 GGTCTC
BsaWI 3427 ACCGGA [AT]^CCGG[AT]
3639 TCCGGA
BsmI 3 GCATTC GAATGC(1/-1)
2343 GCATTC
BstXI 3531 CCAGGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3987 CCAAGGACCTGG
EcoRV 447 GATATC GAT^ATC
880 GATATC
MseI 2357 TTAA T^TAA
2492 TTAA
NdeI 1168 CATATG CA^TATG
2224 CATATG
SacI 27 GAGCTC GAGCT^C
1058 GAGCTC
SfiI 999 GGCCATGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3265 GGCCCCCTTGGCC
SmaI 3334 CCCGGG CCC^GGG
3947 CCCGGG
Tru9I 2357 TTAA T^TAA
2492 TTAA
XcmI 1779 CCACATGGAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2097 CCAGCAGGAGTATGG
XmaCI 3334 CCCGGG C^CCGGG
3947 CCCGGG
XmaI 3334 CCCGGG C^CCGGG
3947 CCCGGG
XmnI 909 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1108 GAAATGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII