Restriction map for
pF1KB7002
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# NO CUT ENZYMES ( 66 enzymes) #
##################################
AccII AflII AgeI AhdI ApaI
AscI AspEI AspI AviII BbeI
BbrPI BbvCI BfrI BglI BsiEI
BsiWI BsrBI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoO65I FseI FspI
KasI KspI MluI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PvuI RsrII SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI Sse8387I Tth111I XmaCI
XmaI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 5474 GACGTC GACGT^C
Acc65I 1383 GGTACC G^GTACC
AccIII 4835 TCCGGA T^CCGGA
AclI 7172 AACGTT AA^CGTT
AcyI 5474 GACGTC G[GA]^CG[CT]C
AocI 4110 CCTTAGG CC^T[ACGT]AGG
Aor51HI 77 AGCGCT AGC^GCT
Asp718I 1383 GGTACC G^GTACC
AvaI 692 CTCGAG C^[CT]CG[GA]G
AvrII 739 CCTAGG C^CTAGG
BlnI 739 CCTAGG C^CTAGG
BlpI 7309 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 7309 GCTAAGC GC^T[ACGT]AGC
BsaAI 5234 TACGTA [CT]AC^GT[GA]
BsaHI 5474 GACGTC G[GA]^CG[CT]C
BseAI 4835 TCCGGA T^CCGGA
BsmBI 5472 GAGACG CGTCTC(1/5)
BsoBI 692 CTCGAG C^[CT]CG[GA]G
BspDI 5254 ATCGAT AT^CGAT
BspEI 4835 TCCGGA T^CCGGA
BssSI 8099 CTCGTG CACGAG(-5/-1)
Bsu36I 4110 CCTTAGG CC^T[ACGT]AGG
CelII 7309 GCTAAGC GC^T[ACGT]AGC
CfoI 78 GCGC GCG^C
ClaI 5254 ATCGAT AT^CGAT
DraIII 10 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 77 AGCGCT AGC^GCT
Eco81I 4110 CCTTAGG CC^T[ACGT]AGG
EcoNI 6 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 4844 CCCGC CCCGC(4/6)
HaeII 77 AGCGCT [GA]GCGC^[CT]
HgaI 220 GCGTC GACGC(5/10)
HhaI 78 GCGC GCG^C
Hin1I 5474 GACGTC G[GA]^CG[CT]C
HinP1I 78 GCGC G^CGC
HpaI 256 GTTAAC GTT^AAC
KpnI 1383 GGTACC GGTAC^C
MroI 4835 TCCGGA T^CCGGA
PaeR7I 692 CTCGAG C^TCGAG
PshAI 8740 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 7172 AACGTT AA^CGTT
SapI 6205 GCTCTTC GCTCTTC(1/4)
SnaBI 5234 TACGTA TAC^GTA
SwaI 443 ATTTAAAT ATTT^AAAT
XbaI 3436 TCTAGA T^CTAGA
XhoI 692 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Alw44I 3152 GTGCAC G^TGCAC
7088 GTGCAC
ApaLI 3152 GTGCAC G^TGCAC
7088 GTGCAC
BanI 1383 GGTACC G^G[CT][GA]CC
7230 GGCACC
BcgI 1848 CGAGGAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
7964 GCATAGCAATCG
BciVI 5694 GGATAC GTATCC(6/5)
8604 GGATAC
BclI 1425 TGATCA T^GATCA
5397 TGATCA
BglII 456 AGATCT A^GATCT
3205 AGATCT
BsaI 4151 GAGACC GGTCTC(1/5)
4115 GGTCTC
BsaWI 1159 ACCGGA [AT]^CCGG[AT]
4835 TCCGGA
BsgI 7 CTGCAC GTGCAG(16/14)
2096 GTGCAG
Bsp1407I 1173 TGTACA T^GTACA
6556 TGTACA
BsrGI 1173 TGTACA T^GTACA
6556 TGTACA
BstXI 6871 CCAGCTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
8652 CCATGACAGTGG
FbaI 1425 TGATCA T^GATCA
5397 TGATCA
HindIII 6837 AAGCTT A^AGCTT
7712 AAGCTT
PpuMI 3272 GGGTCCT [GA]G^G[AT]CC[CT]
7689 AGGTCCT
SexAI 11 ACCAGGT A^CC[AT]GGT
3126 ACCTGGT
SpeI 5698 ACTAGT A^CTAGT
7143 ACTAGT
SspBI 1173 TGTACA T^GTACA
6556 TGTACA
StuI 3133 AGGCCT AGG^CCT
4701 AGGCCT
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# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AspHI AvaII BalI
BamHI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaBI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspHI
BspLU11I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRI
EcoRII EcoRV EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NcoI
NdeI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI PshBI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SphI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI